Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02062 and H281DRAFT_02063 overlap by 4 nucleotides H281DRAFT_02063 and H281DRAFT_02064 are separated by 79 nucleotides H281DRAFT_02064 and H281DRAFT_02065 are separated by 122 nucleotides H281DRAFT_02065 and H281DRAFT_02066 are separated by 232 nucleotides
H281DRAFT_02062: H281DRAFT_02062 - BirA family transcriptional regulator, biotin operon repressor / biotin-[acetyl-CoA-carboxylase] ligase, at 184,590 to 185,504
_02062
H281DRAFT_02063: H281DRAFT_02063 - type III pantothenate kinase, at 185,501 to 186,343
_02063
H281DRAFT_02064: H281DRAFT_02064 - hypothetical protein, at 186,423 to 186,662
_02064
H281DRAFT_02065: H281DRAFT_02065 - rfaE bifunctional protein, domain II, at 186,785 to 187,267
_02065
H281DRAFT_02066: H281DRAFT_02066 - hypothetical protein, at 187,500 to 188,372
_02066
Position (kb) 186
187 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 186.342 kb on - strand at 186.353 kb on + strand at 186.416 kb on + strand at 186.418 kb on + strand at 186.418 kb on + strand at 186.418 kb on + strand at 186.445 kb on + strand at 186.446 kb on - strand at 186.446 kb on - strand at 186.446 kb on - strand at 186.660 kb on + strand at 186.661 kb on - strand at 186.726 kb on - strand at 186.745 kb on + strand at 186.746 kb on - strand at 186.746 kb on - strand at 186.753 kb on - strand at 186.786 kb on + strand at 186.787 kb on - strand at 186.796 kb on + strand at 186.898 kb on + strand, within H281DRAFT_02065 at 186.899 kb on - strand, within H281DRAFT_02065 at 186.899 kb on - strand, within H281DRAFT_02065 at 186.899 kb on - strand, within H281DRAFT_02065 at 186.909 kb on + strand, within H281DRAFT_02065 at 187.140 kb on + strand, within H281DRAFT_02065 at 187.312 kb on - strand at 187.312 kb on - strand at 187.312 kb on - strand at 187.312 kb on - strand at 187.312 kb on - strand at 187.327 kb on + strand at 187.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 186,342 - +1.0 186,353 + +0.2 186,416 + +0.1 186,418 + +0.2 186,418 + -0.1 186,418 + -0.3 186,445 + +0.3 186,446 - +0.6 186,446 - -1.0 186,446 - +0.4 186,660 + +0.1 186,661 - -0.0 186,726 - +0.3 186,745 + -0.3 186,746 - +0.1 186,746 - -0.3 186,753 - +1.2 186,786 + +0.7 186,787 - +0.1 186,796 + -0.5 186,898 + H281DRAFT_02065 0.23 -0.0 186,899 - H281DRAFT_02065 0.24 -1.0 186,899 - H281DRAFT_02065 0.24 -2.5 186,899 - H281DRAFT_02065 0.24 -3.5 186,909 + H281DRAFT_02065 0.26 -1.3 187,140 + H281DRAFT_02065 0.73 -1.0 187,312 - -0.2 187,312 - +0.1 187,312 - +0.1 187,312 - -0.3 187,312 - +0.4 187,327 + -2.2 187,354 - -0.6
Or see this region's nucleotide sequence