Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01389 and H281DRAFT_01390 are separated by 197 nucleotides H281DRAFT_01390 and H281DRAFT_01391 are separated by 22 nucleotides H281DRAFT_01391 and H281DRAFT_01392 overlap by 4 nucleotides H281DRAFT_01392 and H281DRAFT_01393 are separated by 16 nucleotides
H281DRAFT_01389: H281DRAFT_01389 - PAS domain S-box-containing protein, at 240,969 to 243,668
_01389
H281DRAFT_01390: H281DRAFT_01390 - hypothetical protein, at 243,866 to 244,132
_01390
H281DRAFT_01391: H281DRAFT_01391 - two-component system, OmpR family, osmolarity sensor histidine kinase EnvZ, at 244,155 to 245,549
_01391
H281DRAFT_01392: H281DRAFT_01392 - two component transcriptional regulator, winged helix family, at 245,546 to 246,259
_01392
H281DRAFT_01393: H281DRAFT_01393 - hydrophobic/amphiphilic exporter-1, HAE1 family, at 246,276 to 249,407
_01393
Position (kb)
244
245
246 Strain fitness (log2 ratio)
-1
0
1 at 243.435 kb on + strand at 243.727 kb on - strand at 243.728 kb on + strand at 243.729 kb on - strand at 243.729 kb on - strand at 243.790 kb on - strand at 244.010 kb on + strand, within H281DRAFT_01390 at 244.381 kb on + strand, within H281DRAFT_01391 at 244.382 kb on - strand, within H281DRAFT_01391 at 244.382 kb on - strand, within H281DRAFT_01391 at 245.384 kb on - strand, within H281DRAFT_01391 at 245.574 kb on + strand at 245.956 kb on - strand, within H281DRAFT_01392 at 246.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 243,435 + +0.6 243,727 - +1.0 243,728 + -1.3 243,729 - -1.0 243,729 - -0.7 243,790 - +0.0 244,010 + H281DRAFT_01390 0.54 +1.6 244,381 + H281DRAFT_01391 0.16 -1.0 244,382 - H281DRAFT_01391 0.16 -0.7 244,382 - H281DRAFT_01391 0.16 +1.1 245,384 - H281DRAFT_01391 0.88 +0.4 245,574 + +0.7 245,956 - H281DRAFT_01392 0.57 +1.0 246,337 + -0.2
Or see this region's nucleotide sequence