Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01341 and H281DRAFT_01342 are separated by 46 nucleotides H281DRAFT_01342 and H281DRAFT_01343 are separated by 40 nucleotides H281DRAFT_01343 and H281DRAFT_01344 are separated by 72 nucleotides H281DRAFT_01344 and H281DRAFT_01345 are separated by 28 nucleotides
H281DRAFT_01341: H281DRAFT_01341 - Protein of unknown function (DUF1488), at 182,802 to 183,080
_01341
H281DRAFT_01342: H281DRAFT_01342 - ABC-type phosphate/phosphonate transport system, substrate-binding protein, at 183,127 to 183,918
_01342
H281DRAFT_01343: H281DRAFT_01343 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 183,959 to 185,992
_01343
H281DRAFT_01344: H281DRAFT_01344 - methylglutaconyl-CoA hydratase, at 186,065 to 186,850
_01344
H281DRAFT_01345: H281DRAFT_01345 - 3-methylcrotonyl-CoA carboxylase beta subunit, at 186,879 to 188,486
_01345
Position (kb) 183
184
185
186 Strain fitness (log2 ratio) -2
-1
0
1 at 183.081 kb on + strand at 183.081 kb on + strand at 183.094 kb on + strand at 183.095 kb on - strand at 183.106 kb on + strand at 183.154 kb on + strand at 183.154 kb on + strand at 183.466 kb on + strand, within H281DRAFT_01342 at 183.503 kb on + strand, within H281DRAFT_01342 at 183.623 kb on + strand, within H281DRAFT_01342 at 183.689 kb on + strand, within H281DRAFT_01342 at 183.972 kb on - strand at 184.262 kb on - strand, within H281DRAFT_01343 at 184.304 kb on + strand, within H281DRAFT_01343 at 185.389 kb on - strand, within H281DRAFT_01343 at 186.843 kb on + strand at 186.905 kb on + strand at 186.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 183,081 + +0.3 183,081 + -0.9 183,094 + -2.1 183,095 - +0.2 183,106 + -0.4 183,154 + -1.5 183,154 + +0.7 183,466 + H281DRAFT_01342 0.43 +0.0 183,503 + H281DRAFT_01342 0.47 +0.3 183,623 + H281DRAFT_01342 0.63 +0.8 183,689 + H281DRAFT_01342 0.71 -2.1 183,972 - +0.3 184,262 - H281DRAFT_01343 0.15 -0.1 184,304 + H281DRAFT_01343 0.17 -0.5 185,389 - H281DRAFT_01343 0.70 -0.8 186,843 + -0.7 186,905 + -0.7 186,906 - -0.7
Or see this region's nucleotide sequence