Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00381 and H281DRAFT_00382 are separated by 350 nucleotides H281DRAFT_00382 and H281DRAFT_00383 are separated by 22 nucleotides H281DRAFT_00383 and H281DRAFT_00384 overlap by 1 nucleotides
H281DRAFT_00381: H281DRAFT_00381 - dihydropyrimidinase, at 420,983 to 422,434
_00381
H281DRAFT_00382: H281DRAFT_00382 - amino acid ABC transporter substrate-binding protein, PAAT family, at 422,785 to 423,567
_00382
H281DRAFT_00383: H281DRAFT_00383 - amino acid ABC transporter membrane protein, PAAT family, at 423,590 to 424,267
_00383
H281DRAFT_00384: H281DRAFT_00384 - amino acid ABC transporter ATP-binding protein, PAAT family, at 424,267 to 425,034
_00384
Position (kb)
422
423
424 Strain fitness (log2 ratio)
-2
-1
0
1 at 421.817 kb on + strand, within H281DRAFT_00381 at 422.282 kb on - strand, within H281DRAFT_00381 at 422.593 kb on - strand at 422.613 kb on - strand at 422.914 kb on + strand, within H281DRAFT_00382 at 422.915 kb on - strand, within H281DRAFT_00382 at 422.915 kb on - strand, within H281DRAFT_00382 at 423.541 kb on + strand at 423.541 kb on + strand at 423.636 kb on + strand at 423.639 kb on - strand at 423.639 kb on - strand at 423.764 kb on + strand, within H281DRAFT_00383 at 423.897 kb on - strand, within H281DRAFT_00383 at 424.158 kb on - strand, within H281DRAFT_00383 at 424.179 kb on - strand, within H281DRAFT_00383 at 424.389 kb on + strand, within H281DRAFT_00384
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 421,817 + H281DRAFT_00381 0.57 +1.0 422,282 - H281DRAFT_00381 0.89 -0.1 422,593 - +0.7 422,613 - -0.8 422,914 + H281DRAFT_00382 0.16 +1.0 422,915 - H281DRAFT_00382 0.17 +0.2 422,915 - H281DRAFT_00382 0.17 -0.5 423,541 + +0.1 423,541 + -0.2 423,636 + -0.2 423,639 - -1.9 423,639 - -0.4 423,764 + H281DRAFT_00383 0.26 -0.8 423,897 - H281DRAFT_00383 0.45 +0.5 424,158 - H281DRAFT_00383 0.84 -1.5 424,179 - H281DRAFT_00383 0.87 -0.8 424,389 + H281DRAFT_00384 0.16 +0.7
Or see this region's nucleotide sequence