Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00200 and H281DRAFT_00201 are separated by 199 nucleotides H281DRAFT_00201 and H281DRAFT_00202 are separated by 158 nucleotides H281DRAFT_00202 and H281DRAFT_00203 are separated by 12 nucleotides
H281DRAFT_00200: H281DRAFT_00200 - HemY protein, at 218,070 to 219,260
_00200
H281DRAFT_00201: H281DRAFT_00201 - hypothetical protein, at 219,460 to 219,777
_00201
H281DRAFT_00202: H281DRAFT_00202 - RNA polymerase sigma factor, sigma-70 family, at 219,936 to 220,517
_00202
H281DRAFT_00203: H281DRAFT_00203 - hypothetical protein, at 220,530 to 220,838
_00203
Position (kb) 219
220 Strain fitness (log2 ratio) -1
0
1
2
3 at 218.475 kb on + strand at 218.867 kb on - strand, within H281DRAFT_00200 at 219.436 kb on + strand at 219.461 kb on + strand at 219.461 kb on + strand at 219.495 kb on + strand, within H281DRAFT_00201 at 219.496 kb on - strand, within H281DRAFT_00201 at 219.768 kb on - strand at 219.811 kb on + strand at 220.069 kb on + strand, within H281DRAFT_00202 at 220.069 kb on + strand, within H281DRAFT_00202 at 220.070 kb on - strand, within H281DRAFT_00202 at 220.070 kb on - strand, within H281DRAFT_00202 at 220.070 kb on - strand, within H281DRAFT_00202 at 220.296 kb on + strand, within H281DRAFT_00202 at 220.436 kb on + strand at 220.437 kb on - strand, within H281DRAFT_00202 at 220.515 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 218,475 + -0.8 218,867 - H281DRAFT_00200 0.67 -0.2 219,436 + -0.2 219,461 + -0.6 219,461 + -0.2 219,495 + H281DRAFT_00201 0.11 +0.7 219,496 - H281DRAFT_00201 0.11 +1.1 219,768 - +0.5 219,811 + +0.1 220,069 + H281DRAFT_00202 0.23 -0.4 220,069 + H281DRAFT_00202 0.23 +0.6 220,070 - H281DRAFT_00202 0.23 +0.6 220,070 - H281DRAFT_00202 0.23 -0.9 220,070 - H281DRAFT_00202 0.23 +0.2 220,296 + H281DRAFT_00202 0.62 -0.7 220,436 + +3.4 220,437 - H281DRAFT_00202 0.86 -1.2 220,515 + -0.3
Or see this region's nucleotide sequence