Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3279 and Dsui_3280 are separated by 89 nucleotides Dsui_3280 and Dsui_3281 overlap by 4 nucleotides Dsui_3281 and Dsui_3282 are separated by 13 nucleotides
Dsui_3279: Dsui_3279 - formate dehydrogenase family accessory protein FdhD, at 3,516,399 to 3,517,352
_3279
Dsui_3280: Dsui_3280 - formate dehydrogenase, gamma subunit, at 3,517,442 to 3,518,062
_3280
Dsui_3281: Dsui_3281 - formate dehydrogenase, beta subunit, Fe-S containing, at 3,518,059 to 3,518,949
_3281
Dsui_3282: Dsui_3282 - formate dehydrogenase, alpha subunit, at 3,518,963 to 3,522,016
_3282
Position (kb)
3517
3518
3519 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3516.453 kb on + strand at 3516.461 kb on - strand at 3516.472 kb on - strand at 3516.516 kb on + strand at 3516.538 kb on - strand, within Dsui_3279 at 3516.656 kb on + strand at 3516.671 kb on + strand at 3516.679 kb on - strand, within Dsui_3279 at 3516.773 kb on + strand at 3516.781 kb on - strand, within Dsui_3279 at 3517.064 kb on - strand, within Dsui_3279 at 3517.075 kb on - strand, within Dsui_3279 at 3517.090 kb on - strand, within Dsui_3279 at 3517.122 kb on + strand at 3517.130 kb on - strand, within Dsui_3279 at 3517.130 kb on - strand, within Dsui_3279 at 3517.206 kb on + strand at 3517.238 kb on - strand, within Dsui_3279 at 3517.267 kb on - strand at 3517.286 kb on + strand at 3517.361 kb on + strand at 3517.361 kb on + strand at 3517.369 kb on + strand at 3517.369 kb on - strand at 3517.507 kb on - strand, within Dsui_3280 at 3517.552 kb on + strand at 3517.557 kb on + strand at 3517.628 kb on + strand at 3517.628 kb on + strand at 3517.636 kb on - strand, within Dsui_3280 at 3517.691 kb on + strand, within Dsui_3280 at 3517.725 kb on - strand, within Dsui_3280 at 3517.729 kb on - strand, within Dsui_3280 at 3517.786 kb on + strand at 3517.910 kb on - strand, within Dsui_3280 at 3517.951 kb on - strand, within Dsui_3280 at 3517.951 kb on - strand, within Dsui_3280 at 3517.987 kb on + strand, within Dsui_3280 at 3517.987 kb on - strand, within Dsui_3280 at 3518.079 kb on - strand at 3518.107 kb on - strand at 3518.137 kb on - strand at 3518.137 kb on - strand at 3518.154 kb on + strand, within Dsui_3281 at 3518.171 kb on + strand at 3518.179 kb on - strand, within Dsui_3281 at 3518.179 kb on - strand, within Dsui_3281 at 3518.181 kb on + strand, within Dsui_3281 at 3518.183 kb on + strand at 3518.207 kb on - strand, within Dsui_3281 at 3518.249 kb on - strand, within Dsui_3281 at 3518.292 kb on + strand at 3518.416 kb on - strand, within Dsui_3281 at 3518.438 kb on - strand, within Dsui_3281 at 3518.478 kb on - strand, within Dsui_3281 at 3518.478 kb on - strand, within Dsui_3281 at 3518.501 kb on - strand, within Dsui_3281 at 3518.516 kb on - strand, within Dsui_3281 at 3518.616 kb on + strand, within Dsui_3281 at 3518.624 kb on - strand, within Dsui_3281 at 3518.626 kb on + strand at 3518.699 kb on - strand, within Dsui_3281 at 3518.700 kb on + strand, within Dsui_3281 at 3518.708 kb on - strand, within Dsui_3281 at 3518.731 kb on + strand at 3518.731 kb on + strand at 3518.731 kb on + strand at 3518.739 kb on - strand, within Dsui_3281 at 3518.766 kb on + strand, within Dsui_3281 at 3518.879 kb on - strand at 3518.961 kb on + strand at 3518.999 kb on - strand at 3519.042 kb on + strand at 3519.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 3,516,453 + +0.5 3,516,461 - +0.2 3,516,472 - -1.7 3,516,516 + -1.5 3,516,538 - Dsui_3279 0.15 -1.2 3,516,656 + +0.4 3,516,671 + -0.3 3,516,679 - Dsui_3279 0.29 -1.0 3,516,773 + -2.4 3,516,781 - Dsui_3279 0.40 +1.0 3,517,064 - Dsui_3279 0.70 -0.1 3,517,075 - Dsui_3279 0.71 +0.7 3,517,090 - Dsui_3279 0.72 +2.6 3,517,122 + +0.6 3,517,130 - Dsui_3279 0.77 -0.3 3,517,130 - Dsui_3279 0.77 +0.6 3,517,206 + +0.3 3,517,238 - Dsui_3279 0.88 +0.3 3,517,267 - -0.5 3,517,286 + -2.7 3,517,361 + -3.5 3,517,361 + +1.9 3,517,369 + -2.5 3,517,369 - -0.0 3,517,507 - Dsui_3280 0.10 +0.2 3,517,552 + -3.0 3,517,557 + -0.1 3,517,628 + -2.1 3,517,628 + -2.7 3,517,636 - Dsui_3280 0.31 +0.1 3,517,691 + Dsui_3280 0.40 -0.4 3,517,725 - Dsui_3280 0.46 -0.7 3,517,729 - Dsui_3280 0.46 -0.5 3,517,786 + -1.8 3,517,910 - Dsui_3280 0.75 +0.5 3,517,951 - Dsui_3280 0.82 -1.0 3,517,951 - Dsui_3280 0.82 -0.1 3,517,987 + Dsui_3280 0.88 -0.8 3,517,987 - Dsui_3280 0.88 +0.4 3,518,079 - +0.6 3,518,107 - +0.4 3,518,137 - +0.8 3,518,137 - +0.1 3,518,154 + Dsui_3281 0.11 -1.7 3,518,171 + -3.4 3,518,179 - Dsui_3281 0.13 -1.3 3,518,179 - Dsui_3281 0.13 -0.2 3,518,181 + Dsui_3281 0.14 -2.1 3,518,183 + +3.4 3,518,207 - Dsui_3281 0.17 +0.6 3,518,249 - Dsui_3281 0.21 +0.1 3,518,292 + -2.9 3,518,416 - Dsui_3281 0.40 -0.2 3,518,438 - Dsui_3281 0.43 +0.3 3,518,478 - Dsui_3281 0.47 -2.0 3,518,478 - Dsui_3281 0.47 +1.1 3,518,501 - Dsui_3281 0.50 +0.5 3,518,516 - Dsui_3281 0.51 +0.8 3,518,616 + Dsui_3281 0.63 -0.5 3,518,624 - Dsui_3281 0.63 +0.3 3,518,626 + +0.0 3,518,699 - Dsui_3281 0.72 +0.1 3,518,700 + Dsui_3281 0.72 -1.4 3,518,708 - Dsui_3281 0.73 -0.1 3,518,731 + -2.5 3,518,731 + -0.8 3,518,731 + -3.2 3,518,739 - Dsui_3281 0.76 +1.9 3,518,766 + Dsui_3281 0.79 -2.5 3,518,879 - +0.3 3,518,961 + -0.7 3,518,999 - +1.0 3,519,042 + -0.1 3,519,048 - +0.4
Or see this region's nucleotide sequence