Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3197 and Dsui_3198 are separated by 93 nucleotides Dsui_3198 and Dsui_3199 are separated by 142 nucleotides Dsui_3199 and Dsui_3200 overlap by 1 nucleotides Dsui_3200 and Dsui_3201 are separated by 20 nucleotides
Dsui_3197: Dsui_3197 - aspartate-semialdehyde dehydrogenase, gamma-proteobacterial, at 3,433,033 to 3,434,166
_3197
Dsui_3198: Dsui_3198 - 3-isopropylmalate dehydrogenase, at 3,434,260 to 3,435,324
_3198
Dsui_3199: Dsui_3199 - 3-isopropylmalate dehydratase, small subunit, at 3,435,467 to 3,436,105
_3199
Dsui_3200: Dsui_3200 - putative small secreted protein, at 3,436,105 to 3,436,227
_3200
Dsui_3201: Dsui_3201 - 3-isopropylmalate dehydratase, large subunit, at 3,436,248 to 3,437,654
_3201
Position (kb)
3434
3435
3436 Strain fitness (log2 ratio)
-2
-1
0
1 at 3434.206 kb on - strand at 3434.222 kb on + strand at 3434.223 kb on + strand at 3434.231 kb on - strand at 3434.258 kb on + strand at 3435.349 kb on - strand at 3435.373 kb on + strand at 3435.381 kb on - strand at 3436.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 3,434,206 - +0.9 3,434,222 + -0.3 3,434,223 + +0.0 3,434,231 - +1.2 3,434,258 + -0.9 3,435,349 - +1.4 3,435,373 + +0.9 3,435,381 - -2.6 3,436,237 - -0.0
Or see this region's nucleotide sequence