Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1728 and Dsui_1729 are separated by 75 nucleotides Dsui_1729 and Dsui_1730 are separated by 37 nucleotides Dsui_1730 and Dsui_1731 are separated by 108 nucleotides Dsui_1731 and Dsui_1732 are separated by 171 nucleotides
Dsui_1728: Dsui_1728 - flagellar motor protein, at 1,819,246 to 1,820,097
_1728
Dsui_1729: Dsui_1729 - flagellar biosynthesis/type III secretory pathway chaperone, at 1,820,173 to 1,820,640
_1729
Dsui_1730: Dsui_1730 - flagellar biosynthesis anti-sigma factor FlgM, at 1,820,678 to 1,820,968
_1730
Dsui_1731: Dsui_1731 - flagella basal body P-ring formation protein FlgA, at 1,821,077 to 1,821,796
_1731
Dsui_1732: Dsui_1732 - flagellar basal-body rod protein FlgB, at 1,821,968 to 1,822,381
_1732
Position (kb)
1820
1821 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1819.678 kb on + strand, within Dsui_1728 at 1819.686 kb on - strand, within Dsui_1728 at 1819.761 kb on - strand, within Dsui_1728 at 1819.814 kb on + strand, within Dsui_1728 at 1819.839 kb on - strand, within Dsui_1728 at 1819.841 kb on + strand, within Dsui_1728 at 1819.846 kb on + strand, within Dsui_1728 at 1819.959 kb on - strand, within Dsui_1728 at 1820.103 kb on + strand at 1820.110 kb on - strand at 1820.186 kb on + strand at 1820.235 kb on - strand, within Dsui_1729 at 1820.239 kb on + strand, within Dsui_1729 at 1820.247 kb on - strand, within Dsui_1729 at 1820.247 kb on - strand, within Dsui_1729 at 1820.286 kb on + strand, within Dsui_1729 at 1820.341 kb on + strand, within Dsui_1729 at 1820.358 kb on + strand, within Dsui_1729 at 1820.455 kb on + strand, within Dsui_1729 at 1820.467 kb on + strand, within Dsui_1729 at 1820.676 kb on + strand at 1820.684 kb on - strand at 1820.777 kb on - strand, within Dsui_1730 at 1820.777 kb on - strand, within Dsui_1730 at 1820.814 kb on + strand, within Dsui_1730 at 1820.894 kb on - strand, within Dsui_1730 at 1820.992 kb on - strand at 1821.008 kb on + strand at 1821.008 kb on + strand at 1821.016 kb on - strand at 1821.084 kb on - strand at 1821.236 kb on + strand, within Dsui_1731 at 1821.244 kb on - strand, within Dsui_1731 at 1821.385 kb on + strand, within Dsui_1731 at 1821.405 kb on - strand, within Dsui_1731 at 1821.446 kb on + strand, within Dsui_1731 at 1821.475 kb on + strand, within Dsui_1731 at 1821.493 kb on - strand, within Dsui_1731 at 1821.516 kb on - strand, within Dsui_1731 at 1821.589 kb on + strand, within Dsui_1731 at 1821.614 kb on - strand, within Dsui_1731 at 1821.615 kb on - strand, within Dsui_1731 at 1821.639 kb on + strand, within Dsui_1731 at 1821.639 kb on + strand, within Dsui_1731 at 1821.639 kb on + strand, within Dsui_1731 at 1821.647 kb on - strand, within Dsui_1731 at 1821.674 kb on + strand, within Dsui_1731 at 1821.685 kb on - strand, within Dsui_1731 at 1821.697 kb on + strand, within Dsui_1731 at 1821.705 kb on - strand, within Dsui_1731 at 1821.710 kb on + strand, within Dsui_1731 at 1821.710 kb on + strand, within Dsui_1731 at 1821.712 kb on - strand, within Dsui_1731 at 1821.717 kb on - strand, within Dsui_1731 at 1821.751 kb on + strand at 1821.751 kb on + strand at 1821.751 kb on + strand at 1821.751 kb on + strand at 1821.758 kb on + strand at 1821.759 kb on - strand at 1821.759 kb on - strand at 1821.759 kb on - strand at 1821.759 kb on - strand at 1821.759 kb on - strand at 1821.759 kb on - strand at 1821.763 kb on + strand at 1821.777 kb on - strand at 1821.794 kb on - strand at 1821.829 kb on + strand at 1821.856 kb on + strand at 1821.864 kb on - strand at 1821.901 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 1,819,678 + Dsui_1728 0.51 +0.7 1,819,686 - Dsui_1728 0.52 +0.2 1,819,761 - Dsui_1728 0.60 +0.2 1,819,814 + Dsui_1728 0.67 +1.2 1,819,839 - Dsui_1728 0.70 +0.1 1,819,841 + Dsui_1728 0.70 +1.1 1,819,846 + Dsui_1728 0.70 +1.0 1,819,959 - Dsui_1728 0.84 +1.0 1,820,103 + +1.4 1,820,110 - +0.8 1,820,186 + +0.2 1,820,235 - Dsui_1729 0.13 +1.2 1,820,239 + Dsui_1729 0.14 +0.7 1,820,247 - Dsui_1729 0.16 +1.1 1,820,247 - Dsui_1729 0.16 +1.7 1,820,286 + Dsui_1729 0.24 +1.1 1,820,341 + Dsui_1729 0.36 +0.6 1,820,358 + Dsui_1729 0.40 +0.6 1,820,455 + Dsui_1729 0.60 +0.4 1,820,467 + Dsui_1729 0.63 +0.4 1,820,676 + +0.1 1,820,684 - +1.2 1,820,777 - Dsui_1730 0.34 -3.2 1,820,777 - Dsui_1730 0.34 -3.2 1,820,814 + Dsui_1730 0.47 -3.3 1,820,894 - Dsui_1730 0.74 +0.5 1,820,992 - -0.3 1,821,008 + -2.7 1,821,008 + -0.3 1,821,016 - +1.7 1,821,084 - +0.8 1,821,236 + Dsui_1731 0.22 -1.7 1,821,244 - Dsui_1731 0.23 +1.1 1,821,385 + Dsui_1731 0.43 -1.0 1,821,405 - Dsui_1731 0.46 +0.2 1,821,446 + Dsui_1731 0.51 +0.3 1,821,475 + Dsui_1731 0.55 -0.4 1,821,493 - Dsui_1731 0.58 +0.5 1,821,516 - Dsui_1731 0.61 +0.4 1,821,589 + Dsui_1731 0.71 +0.5 1,821,614 - Dsui_1731 0.75 +0.1 1,821,615 - Dsui_1731 0.75 +0.7 1,821,639 + Dsui_1731 0.78 -0.4 1,821,639 + Dsui_1731 0.78 +0.2 1,821,639 + Dsui_1731 0.78 -0.9 1,821,647 - Dsui_1731 0.79 +0.6 1,821,674 + Dsui_1731 0.83 -0.5 1,821,685 - Dsui_1731 0.84 +0.5 1,821,697 + Dsui_1731 0.86 -0.1 1,821,705 - Dsui_1731 0.87 +1.0 1,821,710 + Dsui_1731 0.88 -0.7 1,821,710 + Dsui_1731 0.88 -0.4 1,821,712 - Dsui_1731 0.88 +0.7 1,821,717 - Dsui_1731 0.89 -0.4 1,821,751 + -0.6 1,821,751 + +0.1 1,821,751 + -0.3 1,821,751 + -0.8 1,821,758 + -0.8 1,821,759 - +1.5 1,821,759 - +0.3 1,821,759 - -0.2 1,821,759 - +0.6 1,821,759 - -0.3 1,821,759 - -0.9 1,821,763 + -0.8 1,821,777 - +0.1 1,821,794 - +0.4 1,821,829 + -0.6 1,821,856 + -0.8 1,821,864 - +0.1 1,821,901 + -3.2
Or see this region's nucleotide sequence