Experiment: ICP3_phage_41.1_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_14995 and CSW01_15000 are separated by 43 nucleotides CSW01_15000 and CSW01_15005 overlap by 26 nucleotides CSW01_15005 and CSW01_15010 are separated by 148 nucleotides CSW01_15010 and CSW01_15015 are separated by 189 nucleotides
CSW01_14995: CSW01_14995 - IS200/IS605 family transposase IS1004, at 219,820 to 220,257
_14995
CSW01_15000: CSW01_15000 - rfbP protein, at 220,301 to 220,414
_15000
CSW01_15005: CSW01_15005 - HNH endonuclease, at 220,389 to 220,613
_15005
CSW01_15010: CSW01_15010 - ATP-dependent RNA helicase RhlE, at 220,762 to 222,117
_15010
CSW01_15015: CSW01_15015 - anaerobic C4-dicarboxylate transporter, at 222,307 to 223,635
_15015
Position (kb) 220
221
222
223 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 220.388 kb on + strand, within CSW01_15000 at 220.408 kb on + strand at 220.421 kb on + strand, within CSW01_15005 at 220.443 kb on + strand, within CSW01_15005 at 220.560 kb on + strand, within CSW01_15005 at 220.626 kb on + strand at 220.725 kb on + strand at 220.891 kb on + strand at 220.899 kb on - strand, within CSW01_15010 at 221.098 kb on + strand, within CSW01_15010 at 221.121 kb on + strand, within CSW01_15010 at 221.246 kb on - strand, within CSW01_15010 at 221.262 kb on + strand, within CSW01_15010 at 221.320 kb on - strand, within CSW01_15010 at 221.390 kb on + strand, within CSW01_15010 at 221.460 kb on + strand, within CSW01_15010 at 221.460 kb on + strand, within CSW01_15010 at 221.460 kb on + strand, within CSW01_15010 at 221.460 kb on + strand, within CSW01_15010 at 221.466 kb on - strand, within CSW01_15010 at 221.468 kb on - strand, within CSW01_15010 at 221.468 kb on - strand, within CSW01_15010 at 221.492 kb on - strand, within CSW01_15010 at 221.659 kb on - strand, within CSW01_15010 at 221.758 kb on - strand, within CSW01_15010 at 221.892 kb on - strand, within CSW01_15010 at 221.949 kb on + strand, within CSW01_15010 at 221.957 kb on - strand, within CSW01_15010 at 221.977 kb on + strand, within CSW01_15010 at 222.006 kb on - strand at 222.034 kb on - strand at 222.060 kb on - strand at 222.162 kb on + strand at 222.273 kb on + strand at 222.301 kb on - strand at 222.330 kb on + strand at 222.330 kb on + strand at 222.336 kb on + strand at 222.339 kb on + strand at 222.358 kb on - strand at 222.463 kb on + strand, within CSW01_15015 at 222.466 kb on - strand, within CSW01_15015 at 222.489 kb on - strand, within CSW01_15015 at 222.534 kb on - strand, within CSW01_15015 at 222.556 kb on - strand, within CSW01_15015 at 222.694 kb on + strand, within CSW01_15015 at 222.760 kb on - strand, within CSW01_15015 at 222.766 kb on + strand, within CSW01_15015 at 222.835 kb on + strand, within CSW01_15015 at 222.835 kb on + strand, within CSW01_15015 at 222.843 kb on - strand, within CSW01_15015 at 222.849 kb on + strand, within CSW01_15015 at 222.856 kb on - strand, within CSW01_15015 at 222.925 kb on - strand, within CSW01_15015 at 222.938 kb on - strand, within CSW01_15015 at 223.010 kb on + strand, within CSW01_15015 at 223.012 kb on + strand, within CSW01_15015 at 223.032 kb on + strand, within CSW01_15015
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep1 remove 220,388 + CSW01_15000 0.76 +1.3 220,408 + -1.9 220,421 + CSW01_15005 0.14 -3.8 220,443 + CSW01_15005 0.24 -0.1 220,560 + CSW01_15005 0.76 +0.6 220,626 + +0.4 220,725 + +0.3 220,891 + -2.6 220,899 - CSW01_15010 0.10 -1.9 221,098 + CSW01_15010 0.25 +0.6 221,121 + CSW01_15010 0.26 +0.6 221,246 - CSW01_15010 0.36 +0.4 221,262 + CSW01_15010 0.37 -0.1 221,320 - CSW01_15010 0.41 +0.0 221,390 + CSW01_15010 0.46 +0.1 221,460 + CSW01_15010 0.51 -2.0 221,460 + CSW01_15010 0.51 -0.5 221,460 + CSW01_15010 0.51 +0.1 221,460 + CSW01_15010 0.51 +1.0 221,466 - CSW01_15010 0.52 -1.2 221,468 - CSW01_15010 0.52 -2.6 221,468 - CSW01_15010 0.52 +1.5 221,492 - CSW01_15010 0.54 -0.9 221,659 - CSW01_15010 0.66 +0.5 221,758 - CSW01_15010 0.73 -1.0 221,892 - CSW01_15010 0.83 +1.8 221,949 + CSW01_15010 0.88 +0.0 221,957 - CSW01_15010 0.88 +1.4 221,977 + CSW01_15010 0.90 -0.0 222,006 - +1.3 222,034 - -0.5 222,060 - +0.0 222,162 + +0.1 222,273 + -3.1 222,301 - -0.9 222,330 + -0.1 222,330 + +1.6 222,336 + -2.3 222,339 + +0.5 222,358 - +0.8 222,463 + CSW01_15015 0.12 -0.8 222,466 - CSW01_15015 0.12 -0.6 222,489 - CSW01_15015 0.14 -1.9 222,534 - CSW01_15015 0.17 -3.0 222,556 - CSW01_15015 0.19 -1.4 222,694 + CSW01_15015 0.29 +0.3 222,760 - CSW01_15015 0.34 +1.0 222,766 + CSW01_15015 0.35 +0.1 222,835 + CSW01_15015 0.40 +0.7 222,835 + CSW01_15015 0.40 +0.4 222,843 - CSW01_15015 0.40 -0.1 222,849 + CSW01_15015 0.41 -2.0 222,856 - CSW01_15015 0.41 -1.3 222,925 - CSW01_15015 0.47 +1.5 222,938 - CSW01_15015 0.47 +0.4 223,010 + CSW01_15015 0.53 -0.3 223,012 + CSW01_15015 0.53 -1.0 223,032 + CSW01_15015 0.55 -0.4
Or see this region's nucleotide sequence