Experiment: ICP3_phage_41.1_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_10425 and CSW01_10430 are separated by 28 nucleotides CSW01_10430 and CSW01_10435 are separated by 88 nucleotides CSW01_10435 and CSW01_10440 are separated by 14 nucleotides
CSW01_10425: CSW01_10425 - dihydrolipoyllysine-residue succinyltransferase, at 2,244,173 to 2,245,387
_10425
CSW01_10430: CSW01_10430 - 2-oxoglutarate dehydrogenase subunit E1, at 2,245,416 to 2,248,226
_10430
CSW01_10435: CSW01_10435 - succinate dehydrogenase iron-sulfur subunit, at 2,248,315 to 2,249,025
_10435
CSW01_10440: CSW01_10440 - succinate dehydrogenase flavoprotein subunit, at 2,249,040 to 2,250,806
_10440
Position (kb) 2245
2246
2247
2248
2249 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 2244.985 kb on - strand, within CSW01_10425 at 2245.756 kb on + strand, within CSW01_10430 at 2247.168 kb on - strand, within CSW01_10430 at 2247.901 kb on - strand, within CSW01_10430 at 2248.288 kb on - strand at 2248.461 kb on - strand, within CSW01_10435 at 2248.646 kb on - strand, within CSW01_10435 at 2248.878 kb on - strand, within CSW01_10435 at 2249.074 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep1 remove 2,244,985 - CSW01_10425 0.67 -1.4 2,245,756 + CSW01_10430 0.12 -0.5 2,247,168 - CSW01_10430 0.62 +0.8 2,247,901 - CSW01_10430 0.88 -0.2 2,248,288 - -0.6 2,248,461 - CSW01_10435 0.21 -2.0 2,248,646 - CSW01_10435 0.47 -3.2 2,248,878 - CSW01_10435 0.79 -1.0 2,249,074 + -2.5
Or see this region's nucleotide sequence