Experiment: ICP3_phage_41.1_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_09205 and CSW01_09210 are separated by 2 nucleotides CSW01_09210 and CSW01_09215 overlap by 11 nucleotides CSW01_09215 and CSW01_09220 are separated by 108 nucleotides CSW01_09220 and CSW01_09225 overlap by 8 nucleotides CSW01_09225 and CSW01_09230 are separated by 12 nucleotides
CSW01_09205: CSW01_09205 - protein TolR, at 1,978,337 to 1,978,777
_09205
CSW01_09210: CSW01_09210 - protein TolQ, at 1,978,780 to 1,979,463
_09210
CSW01_09215: CSW01_09215 - tol-pal system-associated acyl-CoA thioesterase, at 1,979,453 to 1,979,920
_09215
CSW01_09220: CSW01_09220 - cyd operon protein YbgE, at 1,980,029 to 1,980,346
_09220
CSW01_09225: CSW01_09225 - cytochrome bd-I oxidase subunit CydX, at 1,980,339 to 1,980,446
_09225
CSW01_09230: CSW01_09230 - cytochrome d ubiquinol oxidase subunit II, at 1,980,459 to 1,981,595
_09230
Position (kb)
1979
1980 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1979.309 kb on - strand, within CSW01_09210 at 1979.535 kb on + strand, within CSW01_09215 at 1979.535 kb on + strand, within CSW01_09215 at 1979.723 kb on - strand, within CSW01_09215 at 1979.823 kb on + strand, within CSW01_09215 at 1979.925 kb on + strand at 1979.925 kb on + strand at 1979.928 kb on - strand at 1979.928 kb on - strand at 1979.928 kb on - strand at 1979.933 kb on - strand at 1979.933 kb on - strand at 1979.933 kb on - strand at 1979.988 kb on + strand at 1980.283 kb on - strand, within CSW01_09220 at 1980.293 kb on + strand, within CSW01_09220 at 1980.457 kb on + strand at 1980.465 kb on - strand at 1980.465 kb on - strand at 1980.465 kb on - strand at 1980.465 kb on - strand at 1980.465 kb on - strand at 1980.465 kb on - strand at 1980.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep1 remove 1,979,309 - CSW01_09210 0.77 -1.3 1,979,535 + CSW01_09215 0.18 -3.8 1,979,535 + CSW01_09215 0.18 -2.0 1,979,723 - CSW01_09215 0.58 +0.9 1,979,823 + CSW01_09215 0.79 -1.3 1,979,925 + -4.2 1,979,925 + -0.6 1,979,928 - -2.3 1,979,928 - +0.6 1,979,928 - +0.4 1,979,933 - -1.4 1,979,933 - +0.4 1,979,933 - -0.5 1,979,988 + +0.1 1,980,283 - CSW01_09220 0.80 +0.3 1,980,293 + CSW01_09220 0.83 -2.5 1,980,457 + -2.0 1,980,465 - -2.2 1,980,465 - -0.7 1,980,465 - -1.0 1,980,465 - -2.4 1,980,465 - -0.5 1,980,465 - -1.1 1,980,465 - -2.5
Or see this region's nucleotide sequence