Experiment: ICP3_phage_41.1_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_03575 and CSW01_03580 are separated by 15 nucleotides CSW01_03580 and CSW01_03585 are separated by 162 nucleotides CSW01_03585 and CSW01_03590 are separated by 46 nucleotides
CSW01_03575: CSW01_03575 - isoleucine--tRNA ligase, at 727,431 to 730,280
_03575
CSW01_03580: CSW01_03580 - lipoprotein signal peptidase, at 730,296 to 730,811
_03580
CSW01_03585: CSW01_03585 - peptidylprolyl isomerase, at 730,974 to 731,408
_03585
CSW01_03590: CSW01_03590 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 731,455 to 732,405
_03590
Position (kb) 730
731 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 730.821 kb on - strand at 730.826 kb on - strand at 730.826 kb on - strand at 730.994 kb on - strand at 730.996 kb on - strand at 731.048 kb on - strand, within CSW01_03585 at 731.048 kb on - strand, within CSW01_03585 at 731.194 kb on + strand, within CSW01_03585 at 731.202 kb on - strand, within CSW01_03585 at 731.354 kb on + strand, within CSW01_03585 at 731.362 kb on - strand, within CSW01_03585 at 731.364 kb on + strand, within CSW01_03585 at 731.364 kb on + strand, within CSW01_03585 at 731.377 kb on + strand at 731.393 kb on - strand at 731.453 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep1 remove 730,821 - -0.2 730,826 - -3.3 730,826 - -2.4 730,994 - -2.5 730,996 - -0.0 731,048 - CSW01_03585 0.17 -3.5 731,048 - CSW01_03585 0.17 +0.9 731,194 + CSW01_03585 0.51 -2.8 731,202 - CSW01_03585 0.52 -2.2 731,354 + CSW01_03585 0.87 +1.8 731,362 - CSW01_03585 0.89 -0.2 731,364 + CSW01_03585 0.90 +1.1 731,364 + CSW01_03585 0.90 -2.6 731,377 + -0.5 731,393 - +0.3 731,453 - -1.0
Or see this region's nucleotide sequence