Experiment: ICP3_phage_41.1_MOI_rep1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_00300 and CSW01_00305 are separated by 57 nucleotides CSW01_00305 and CSW01_00310 are separated by 280 nucleotides CSW01_00310 and CSW01_00315 are separated by 107 nucleotides CSW01_00315 and CSW01_00320 are separated by 25 nucleotides CSW01_00320 and CSW01_00325 are separated by 29 nucleotides CSW01_00325 and CSW01_00330 are separated by 232 nucleotides
CSW01_00300: CSW01_00300 - 16S ribosomal RNA, at 53,815 to 55,364
_00300
CSW01_00305: CSW01_00305 - tRNA-Ala, at 55,422 to 55,497
_00305
CSW01_00310: CSW01_00310 - 23S ribosomal RNA, at 55,778 to 58,693
_00310
CSW01_00315: CSW01_00315 - 5S ribosomal RNA, at 58,801 to 58,916
_00315
CSW01_00320: CSW01_00320 - tRNA-Asp, at 58,942 to 59,018
_00320
CSW01_00325: CSW01_00325 - tRNA-Trp, at 59,048 to 59,124
_00325
CSW01_00330: CSW01_00330 - fluoride efflux transporter CrcB, at 59,357 to 59,737
_00330
Position (kb) 55
56
57
58
59 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 59.285 kb on - strand at 59.313 kb on - strand at 59.384 kb on + strand at 59.384 kb on + strand at 59.384 kb on + strand at 59.538 kb on + strand, within CSW01_00330 at 59.539 kb on + strand, within CSW01_00330 at 59.539 kb on + strand, within CSW01_00330 at 59.544 kb on + strand, within CSW01_00330 at 59.547 kb on - strand, within CSW01_00330 at 59.552 kb on - strand, within CSW01_00330 at 59.682 kb on - strand, within CSW01_00330
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep1 remove 59,285 - -1.5 59,313 - +1.5 59,384 + +0.2 59,384 + -1.1 59,384 + -0.1 59,538 + CSW01_00330 0.48 -0.1 59,539 + CSW01_00330 0.48 -0.3 59,539 + CSW01_00330 0.48 -4.3 59,544 + CSW01_00330 0.49 -3.8 59,547 - CSW01_00330 0.50 -1.4 59,552 - CSW01_00330 0.51 +0.8 59,682 - CSW01_00330 0.85 -3.3
Or see this region's nucleotide sequence