Experiment: Gentamicin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_04490 and AO353_04495 are separated by 124 nucleotides AO353_04495 and AO353_04500 are separated by 49 nucleotides AO353_04500 and AO353_04505 are separated by 201 nucleotides
AO353_04490: AO353_04490 - murein transglycosylase, at 968,939 to 970,399
_04490
AO353_04495: AO353_04495 - pyridoxine 5'-phosphate synthase, at 970,524 to 971,267
_04495
AO353_04500: AO353_04500 - DNA repair protein RecO, at 971,317 to 972,006
_04500
AO353_04505: AO353_04505 - GTPase Era, at 972,208 to 973,110
_04505
Position (kb) 971
972
973 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 970.397 kb on + strand at 970.398 kb on - strand at 971.307 kb on + strand at 971.307 kb on + strand at 971.308 kb on - strand at 971.486 kb on + strand, within AO353_04500 at 971.486 kb on + strand, within AO353_04500 at 971.541 kb on + strand, within AO353_04500 at 971.669 kb on + strand, within AO353_04500 at 971.735 kb on + strand, within AO353_04500 at 971.736 kb on - strand, within AO353_04500 at 971.736 kb on - strand, within AO353_04500 at 971.941 kb on - strand at 971.941 kb on - strand at 971.961 kb on + strand at 971.972 kb on + strand at 971.973 kb on - strand at 971.973 kb on - strand at 971.973 kb on - strand at 971.973 kb on - strand at 971.973 kb on - strand at 972.210 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gentamicin 0.006 mg/ml remove 970,397 + -1.9 970,398 - -0.3 971,307 + +0.1 971,307 + +1.1 971,308 - +0.4 971,486 + AO353_04500 0.24 +0.1 971,486 + AO353_04500 0.24 -0.5 971,541 + AO353_04500 0.32 -0.1 971,669 + AO353_04500 0.51 -1.9 971,735 + AO353_04500 0.61 -0.7 971,736 - AO353_04500 0.61 +0.5 971,736 - AO353_04500 0.61 -0.9 971,941 - -1.9 971,941 - +0.5 971,961 + -0.5 971,972 + +0.1 971,973 - -0.9 971,973 - -1.0 971,973 - +0.4 971,973 - +3.1 971,973 - +1.1 972,210 - +1.1
Or see this region's nucleotide sequence