Experiment: Choline chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpdB and Shewana3_1586 are separated by 691 nucleotides Shewana3_1586 and Shewana3_1587 are separated by 225 nucleotides
Shewana3_1585: cpdB - bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein (RefSeq), at 1,859,839 to 1,861,896
cpdB
Shewana3_1586: Shewana3_1586 - peptidyl-dipeptidase Dcp (RefSeq), at 1,862,588 to 1,864,735
_1586
Shewana3_1587: Shewana3_1587 - prolyl 4-hydroxylase, alpha subunit (RefSeq), at 1,864,961 to 1,865,584
_1587
Position (kb)
1862
1863
1864
1865 Strain fitness (log2 ratio)
-1
0
1
2 at 1861.622 kb on + strand, within cpdB at 1861.630 kb on - strand, within cpdB at 1861.630 kb on - strand, within cpdB at 1861.787 kb on - strand at 1861.960 kb on - strand at 1861.982 kb on + strand at 1862.364 kb on + strand at 1862.562 kb on + strand at 1862.737 kb on - strand at 1862.765 kb on + strand at 1863.299 kb on - strand, within Shewana3_1586 at 1863.363 kb on - strand, within Shewana3_1586 at 1863.622 kb on - strand, within Shewana3_1586 at 1863.742 kb on - strand, within Shewana3_1586 at 1863.842 kb on - strand, within Shewana3_1586 at 1863.890 kb on - strand, within Shewana3_1586 at 1863.922 kb on + strand, within Shewana3_1586 at 1863.922 kb on + strand, within Shewana3_1586 at 1863.930 kb on - strand, within Shewana3_1586 at 1863.930 kb on - strand, within Shewana3_1586 at 1863.949 kb on + strand, within Shewana3_1586 at 1863.963 kb on - strand, within Shewana3_1586 at 1864.006 kb on + strand, within Shewana3_1586 at 1864.038 kb on + strand at 1864.671 kb on - strand at 1864.904 kb on + strand at 1864.904 kb on + strand at 1864.922 kb on - strand at 1864.993 kb on - strand at 1865.130 kb on + strand, within Shewana3_1587 at 1865.512 kb on + strand, within Shewana3_1587 at 1865.560 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 300 mM remove 1,861,622 + cpdB Shewana3_1585 0.87 +0.1 1,861,630 - cpdB Shewana3_1585 0.87 +0.1 1,861,630 - cpdB Shewana3_1585 0.87 +1.7 1,861,787 - +0.1 1,861,960 - -0.5 1,861,982 + +0.1 1,862,364 + +0.1 1,862,562 + -1.5 1,862,737 - +0.7 1,862,765 + +0.1 1,863,299 - Shewana3_1586 0.33 -0.9 1,863,363 - Shewana3_1586 0.36 +2.4 1,863,622 - Shewana3_1586 0.48 +0.7 1,863,742 - Shewana3_1586 0.54 +0.1 1,863,842 - Shewana3_1586 0.58 +0.1 1,863,890 - Shewana3_1586 0.61 -0.9 1,863,922 + Shewana3_1586 0.62 -0.1 1,863,922 + Shewana3_1586 0.62 +1.0 1,863,930 - Shewana3_1586 0.62 +0.1 1,863,930 - Shewana3_1586 0.62 +0.1 1,863,949 + Shewana3_1586 0.63 -0.6 1,863,963 - Shewana3_1586 0.64 +1.7 1,864,006 + Shewana3_1586 0.66 +0.7 1,864,038 + +1.1 1,864,671 - +0.1 1,864,904 + +0.1 1,864,904 + +0.7 1,864,922 - +0.7 1,864,993 - +1.7 1,865,130 + Shewana3_1587 0.27 +0.1 1,865,512 + Shewana3_1587 0.88 +0.7 1,865,560 + +0.2
Or see this region's nucleotide sequence