Experiment: Choline chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1542 and folX are separated by 73 nucleotides folX and Shewana3_1544 are separated by 97 nucleotides Shewana3_1544 and Shewana3_1545 are separated by 6 nucleotides
Shewana3_1542: Shewana3_1542 - hypothetical protein (RefSeq), at 1,799,862 to 1,800,752
_1542
Shewana3_1543: folX - D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase (RefSeq), at 1,800,826 to 1,801,191
folX
Shewana3_1544: Shewana3_1544 - hypothetical protein (RefSeq), at 1,801,289 to 1,802,395
_1544
Shewana3_1545: Shewana3_1545 - hypothetical protein (RefSeq), at 1,802,402 to 1,803,313
_1545
Position (kb)
1801
1802
1803 Strain fitness (log2 ratio)
-2
-1
0
1 at 1800.526 kb on + strand, within Shewana3_1542 at 1800.526 kb on + strand, within Shewana3_1542 at 1800.607 kb on - strand, within Shewana3_1542 at 1800.871 kb on + strand, within folX at 1801.395 kb on + strand at 1801.487 kb on - strand, within Shewana3_1544 at 1801.690 kb on + strand, within Shewana3_1544 at 1801.693 kb on - strand, within Shewana3_1544 at 1801.890 kb on - strand, within Shewana3_1544 at 1801.910 kb on + strand, within Shewana3_1544 at 1801.968 kb on - strand, within Shewana3_1544 at 1802.094 kb on - strand, within Shewana3_1544 at 1802.366 kb on + strand at 1802.384 kb on + strand at 1802.541 kb on + strand, within Shewana3_1545 at 1802.826 kb on - strand, within Shewana3_1545 at 1803.018 kb on + strand, within Shewana3_1545 at 1803.026 kb on - strand, within Shewana3_1545 at 1803.300 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 300 mM remove 1,800,526 + Shewana3_1542 0.75 -1.3 1,800,526 + Shewana3_1542 0.75 +0.1 1,800,607 - Shewana3_1542 0.84 +0.1 1,800,871 + folX Shewana3_1543 0.12 +0.2 1,801,395 + +0.6 1,801,487 - Shewana3_1544 0.18 +0.1 1,801,690 + Shewana3_1544 0.36 -1.7 1,801,693 - Shewana3_1544 0.36 +0.1 1,801,890 - Shewana3_1544 0.54 +0.1 1,801,910 + Shewana3_1544 0.56 -0.6 1,801,968 - Shewana3_1544 0.61 -1.9 1,802,094 - Shewana3_1544 0.73 -0.9 1,802,366 + +0.1 1,802,384 + -1.1 1,802,541 + Shewana3_1545 0.15 -0.4 1,802,826 - Shewana3_1545 0.46 +0.7 1,803,018 + Shewana3_1545 0.68 -0.2 1,803,026 - Shewana3_1545 0.68 +0.1 1,803,300 + +0.5
Or see this region's nucleotide sequence