Experiment: Choline chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1125 and recA are separated by 382 nucleotides recA and Shewana3_1127 are separated by 113 nucleotides Shewana3_1127 and alaS are separated by 275 nucleotides
Shewana3_1125: Shewana3_1125 - DNA mismatch repair protein MutS (RefSeq), at 1,323,429 to 1,326,014
_1125
Shewana3_1126: recA - recombinase A (RefSeq), at 1,326,397 to 1,327,470
recA
Shewana3_1127: Shewana3_1127 - regulatory protein RecX (RefSeq), at 1,327,584 to 1,327,955
_1127
Shewana3_1128: alaS - alanyl-tRNA synthetase (RefSeq), at 1,328,231 to 1,330,855
alaS
Position (kb)
1326
1327
1328 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1325.700 kb on - strand, within Shewana3_1125 at 1325.775 kb on + strand at 1325.775 kb on + strand at 1325.783 kb on - strand at 1325.872 kb on + strand at 1325.892 kb on - strand at 1325.894 kb on + strand at 1325.902 kb on - strand at 1325.902 kb on - strand at 1326.052 kb on + strand at 1326.154 kb on + strand at 1326.261 kb on + strand at 1326.263 kb on + strand at 1326.269 kb on - strand at 1326.294 kb on - strand at 1326.377 kb on + strand at 1326.377 kb on + strand at 1326.385 kb on - strand at 1326.385 kb on - strand at 1326.476 kb on + strand at 1326.588 kb on + strand, within recA at 1326.626 kb on + strand, within recA at 1326.634 kb on - strand, within recA at 1326.942 kb on + strand, within recA at 1327.047 kb on + strand, within recA at 1327.047 kb on + strand, within recA at 1327.146 kb on - strand, within recA at 1327.294 kb on + strand, within recA at 1327.294 kb on + strand, within recA at 1327.302 kb on - strand, within recA at 1327.302 kb on - strand, within recA at 1327.366 kb on - strand at 1327.401 kb on + strand at 1327.464 kb on + strand at 1327.964 kb on - strand at 1327.976 kb on + strand at 1327.981 kb on + strand at 1328.124 kb on + strand at 1328.124 kb on + strand at 1328.124 kb on + strand at 1328.132 kb on - strand at 1328.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 300 mM remove 1,325,700 - Shewana3_1125 0.88 -0.2 1,325,775 + +0.3 1,325,775 + -0.7 1,325,783 - +0.0 1,325,872 + +0.3 1,325,892 - -0.2 1,325,894 + +1.0 1,325,902 - +0.3 1,325,902 - -1.0 1,326,052 + -0.1 1,326,154 + -0.1 1,326,261 + -1.1 1,326,263 + -3.2 1,326,269 - -0.9 1,326,294 - -0.8 1,326,377 + -4.6 1,326,377 + -6.1 1,326,385 - -2.0 1,326,385 - +0.3 1,326,476 + -3.8 1,326,588 + recA Shewana3_1126 0.18 -6.3 1,326,626 + recA Shewana3_1126 0.21 -5.6 1,326,634 - recA Shewana3_1126 0.22 +0.3 1,326,942 + recA Shewana3_1126 0.51 -7.4 1,327,047 + recA Shewana3_1126 0.61 -7.1 1,327,047 + recA Shewana3_1126 0.61 -6.0 1,327,146 - recA Shewana3_1126 0.70 -0.7 1,327,294 + recA Shewana3_1126 0.84 -4.8 1,327,294 + recA Shewana3_1126 0.84 -6.0 1,327,302 - recA Shewana3_1126 0.84 +0.3 1,327,302 - recA Shewana3_1126 0.84 +0.3 1,327,366 - +0.3 1,327,401 + -0.4 1,327,464 + -0.8 1,327,964 - +0.3 1,327,976 + -0.1 1,327,981 + -1.1 1,328,124 + +0.3 1,328,124 + -0.3 1,328,124 + -0.4 1,328,132 - +0.3 1,328,132 - +0.1
Or see this region's nucleotide sequence