Experiment: Choline chloride 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4141 and Shewana3_4142 are separated by 242 nucleotides Shewana3_4142 and Shewana3_4143 are separated by 83 nucleotides Shewana3_4143 and Shewana3_4144 are separated by 43 nucleotides Shewana3_4144 and Shewana3_4145 are separated by 36 nucleotides
Shewana3_4141: Shewana3_4141 - putative transposase (RefSeq), at 1,030 to 1,248
_4141
Shewana3_4142: Shewana3_4142 - IS4 family transposase (RefSeq), at 1,491 to 2,471
_4142
Shewana3_4143: Shewana3_4143 - hypothetical protein (RefSeq), at 2,555 to 2,836
_4143
Shewana3_4144: Shewana3_4144 - plastocyanin domain-containing protein (RefSeq), at 2,880 to 3,239
_4144
Shewana3_4145: Shewana3_4145 - copper-translocating P-type ATPase (RefSeq), at 3,276 to 5,612
_4145
Position (kb) 1
2
3 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 0.491 kb on - strand at 0.543 kb on + strand at 0.543 kb on + strand at 0.545 kb on + strand at 0.545 kb on + strand at 0.551 kb on - strand at 0.566 kb on - strand at 0.568 kb on + strand at 0.576 kb on - strand at 0.585 kb on - strand at 0.652 kb on + strand at 0.652 kb on + strand at 0.652 kb on + strand at 0.660 kb on - strand at 0.660 kb on - strand at 0.660 kb on - strand at 0.660 kb on - strand at 0.769 kb on + strand at 0.769 kb on + strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.777 kb on - strand at 0.807 kb on - strand at 0.808 kb on - strand at 0.808 kb on - strand at 0.826 kb on - strand at 0.835 kb on - strand at 0.836 kb on - strand at 0.841 kb on + strand at 0.843 kb on - strand at 0.857 kb on + strand at 0.857 kb on + strand at 0.865 kb on - strand at 0.865 kb on - strand at 0.865 kb on - strand at 0.865 kb on - strand at 0.929 kb on - strand at 0.929 kb on - strand at 0.929 kb on - strand at 0.938 kb on - strand at 0.960 kb on + strand at 0.968 kb on - strand at 0.975 kb on + strand at 0.975 kb on + strand at 0.983 kb on - strand at 0.983 kb on - strand at 0.983 kb on - strand at 0.988 kb on + strand at 0.996 kb on - strand at 0.999 kb on - strand at 1.040 kb on - strand at 1.040 kb on - strand at 1.102 kb on + strand, within Shewana3_4141 at 1.138 kb on - strand, within Shewana3_4141 at 1.138 kb on - strand, within Shewana3_4141 at 1.156 kb on + strand, within Shewana3_4141 at 1.269 kb on + strand at 1.279 kb on + strand at 1.321 kb on - strand at 1.323 kb on + strand at 1.596 kb on - strand, within Shewana3_4142 at 1.689 kb on - strand, within Shewana3_4142 at 1.980 kb on - strand, within Shewana3_4142 at 2.130 kb on - strand, within Shewana3_4142 at 2.130 kb on - strand, within Shewana3_4142 at 2.181 kb on - strand, within Shewana3_4142 at 2.252 kb on + strand, within Shewana3_4142 at 2.276 kb on - strand, within Shewana3_4142 at 2.337 kb on - strand, within Shewana3_4142 at 2.352 kb on - strand, within Shewana3_4142 at 2.545 kb on - strand at 2.898 kb on + strand at 2.903 kb on + strand at 2.906 kb on - strand at 3.106 kb on + strand, within Shewana3_4144 at 3.209 kb on + strand at 3.231 kb on - strand at 3.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 300 mM remove 491 - -1.3 543 + -0.3 543 + -0.3 545 + -0.3 545 + -0.1 551 - -1.3 566 - -0.0 568 + -0.3 576 - -0.4 585 - +0.7 652 + -0.3 652 + +1.4 652 + -0.3 660 - -0.9 660 - -0.8 660 - -1.3 660 - -0.3 769 + -0.3 769 + +0.6 777 - -0.3 777 - -0.7 777 - -0.3 777 - -0.3 777 - -1.2 777 - -3.1 807 - -1.0 808 - +0.1 808 - -0.3 826 - -0.3 835 - -1.9 836 - -0.9 841 + -0.3 843 - -0.3 857 + +0.2 857 + -2.7 865 - +0.8 865 - -0.3 865 - -0.9 865 - +0.6 929 - -0.6 929 - +0.2 929 - -0.5 938 - +0.3 960 + +0.8 968 - -1.0 975 + -1.3 975 + -0.3 983 - +0.1 983 - -0.7 983 - +0.6 988 + -2.6 996 - +0.6 999 - +0.3 1,040 - -0.1 1,040 - +1.0 1,102 + Shewana3_4141 0.33 +0.6 1,138 - Shewana3_4141 0.49 +0.5 1,138 - Shewana3_4141 0.49 -0.2 1,156 + Shewana3_4141 0.58 -0.2 1,269 + -0.3 1,279 + +0.7 1,321 - +0.1 1,323 + -0.3 1,596 - Shewana3_4142 0.11 +0.0 1,689 - Shewana3_4142 0.20 +0.0 1,980 - Shewana3_4142 0.50 -1.0 2,130 - Shewana3_4142 0.65 -0.5 2,130 - Shewana3_4142 0.65 -0.7 2,181 - Shewana3_4142 0.70 +1.0 2,252 + Shewana3_4142 0.78 +0.2 2,276 - Shewana3_4142 0.80 -0.3 2,337 - Shewana3_4142 0.86 -0.7 2,352 - Shewana3_4142 0.88 -0.3 2,545 - +4.2 2,898 + -0.3 2,903 + +0.4 2,906 - -0.4 3,106 + Shewana3_4144 0.63 -2.1 3,209 + -2.3 3,231 - -1.5 3,321 - +0.9
Or see this region's nucleotide sequence