Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05929 and H281DRAFT_05930 are separated by 247 nucleotides H281DRAFT_05930 and H281DRAFT_05931 are separated by 224 nucleotides
H281DRAFT_05929: H281DRAFT_05929 - Pimeloyl-ACP methyl ester carboxylesterase, at 65,961 to 66,749
_05929
H281DRAFT_05930: H281DRAFT_05930 - rod shape-determining protein MreB, at 66,997 to 68,049
_05930
H281DRAFT_05931: H281DRAFT_05931 - phospholipase/carboxylesterase, at 68,274 to 68,933
_05931
Position (kb) 66
67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 65.999 kb on - strand at 66.182 kb on - strand, within H281DRAFT_05929 at 66.444 kb on + strand, within H281DRAFT_05929 at 66.444 kb on + strand, within H281DRAFT_05929 at 66.445 kb on - strand, within H281DRAFT_05929 at 66.445 kb on - strand, within H281DRAFT_05929 at 66.644 kb on - strand, within H281DRAFT_05929 at 66.742 kb on + strand at 66.789 kb on - strand at 66.910 kb on + strand at 66.910 kb on + strand at 66.910 kb on + strand at 66.910 kb on + strand at 66.911 kb on - strand at 66.911 kb on - strand at 66.911 kb on - strand at 66.920 kb on + strand at 66.998 kb on + strand at 66.999 kb on - strand at 67.379 kb on - strand, within H281DRAFT_05930 at 67.416 kb on - strand, within H281DRAFT_05930 at 67.430 kb on + strand, within H281DRAFT_05930 at 67.431 kb on - strand, within H281DRAFT_05930 at 67.485 kb on - strand, within H281DRAFT_05930 at 67.958 kb on + strand at 68.049 kb on + strand at 68.248 kb on + strand at 68.951 kb on + strand at 69.003 kb on - strand at 69.034 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C) remove 65,999 - +0.7 66,182 - H281DRAFT_05929 0.28 +0.0 66,444 + H281DRAFT_05929 0.61 +0.1 66,444 + H281DRAFT_05929 0.61 -0.2 66,445 - H281DRAFT_05929 0.61 +0.6 66,445 - H281DRAFT_05929 0.61 -0.5 66,644 - H281DRAFT_05929 0.87 +0.2 66,742 + +0.9 66,789 - +0.1 66,910 + -0.4 66,910 + +0.8 66,910 + -1.1 66,910 + -0.7 66,911 - -0.6 66,911 - -0.2 66,911 - -3.4 66,920 + +0.3 66,998 + +0.6 66,999 - -1.1 67,379 - H281DRAFT_05930 0.36 -0.6 67,416 - H281DRAFT_05930 0.40 +0.2 67,430 + H281DRAFT_05930 0.41 -0.2 67,431 - H281DRAFT_05930 0.41 -0.7 67,485 - H281DRAFT_05930 0.46 -0.3 67,958 + -0.6 68,049 + -0.0 68,248 + +0.6 68,951 + +0.4 69,003 - -0.7 69,034 + +0.7
Or see this region's nucleotide sequence