Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05721 and H281DRAFT_05722 are separated by 1 nucleotides H281DRAFT_05722 and H281DRAFT_05723 are separated by 53 nucleotides H281DRAFT_05723 and H281DRAFT_05724 are separated by 121 nucleotides
H281DRAFT_05721: H281DRAFT_05721 - acyl-CoA thioesterase, at 140,556 to 141,044
_05721
H281DRAFT_05722: H281DRAFT_05722 - 1,2-epoxyphenylacetyl-CoA isomerase (EC 5.3.3.18) (from data) , at 141,046 to 141,837
_05722
H281DRAFT_05723: H281DRAFT_05723 - bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (from data) , at 141,891 to 143,093
_05723
H281DRAFT_05724: H281DRAFT_05724 - 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase PaaZ (EC:1.2.1.91) (from data) , at 143,215 to 144,915
_05724
Position (kb)
141
142
143
144 Strain fitness (log2 ratio)
-1
0
1
2
3 at 140.912 kb on - strand, within H281DRAFT_05721 at 140.972 kb on - strand, within H281DRAFT_05721 at 140.972 kb on - strand, within H281DRAFT_05721 at 141.025 kb on + strand at 141.025 kb on + strand at 141.044 kb on + strand at 141.045 kb on - strand at 141.045 kb on - strand at 141.045 kb on - strand at 141.104 kb on + strand at 141.104 kb on + strand at 141.104 kb on + strand at 141.199 kb on - strand, within H281DRAFT_05722 at 141.199 kb on - strand, within H281DRAFT_05722 at 141.199 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.295 kb on - strand, within H281DRAFT_05722 at 141.353 kb on - strand, within H281DRAFT_05722 at 141.527 kb on - strand, within H281DRAFT_05722 at 141.791 kb on - strand at 141.830 kb on + strand at 141.880 kb on + strand at 141.880 kb on + strand at 141.880 kb on + strand at 141.881 kb on - strand at 141.884 kb on + strand at 141.884 kb on + strand at 141.884 kb on + strand at 141.884 kb on + strand at 141.885 kb on - strand at 141.885 kb on - strand at 141.885 kb on - strand at 141.907 kb on - strand at 142.276 kb on + strand, within H281DRAFT_05723 at 142.276 kb on + strand, within H281DRAFT_05723 at 142.353 kb on + strand, within H281DRAFT_05723 at 142.354 kb on - strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.837 kb on - strand, within H281DRAFT_05723 at 142.933 kb on + strand, within H281DRAFT_05723 at 142.933 kb on + strand, within H281DRAFT_05723 at 142.934 kb on - strand, within H281DRAFT_05723 at 142.934 kb on - strand, within H281DRAFT_05723 at 142.934 kb on - strand, within H281DRAFT_05723 at 142.974 kb on - strand at 143.078 kb on + strand at 143.078 kb on + strand at 143.080 kb on + strand at 143.081 kb on - strand at 143.094 kb on - strand at 143.094 kb on - strand at 143.105 kb on + strand at 143.144 kb on + strand at 143.144 kb on + strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.184 kb on + strand at 143.185 kb on - strand at 143.185 kb on - strand at 143.207 kb on + strand at 143.207 kb on + strand at 143.225 kb on - strand at 143.401 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.996 kb on + strand, within H281DRAFT_05724 at 143.996 kb on + strand, within H281DRAFT_05724
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C) remove 140,912 - H281DRAFT_05721 0.73 +1.8 140,972 - H281DRAFT_05721 0.85 +1.1 140,972 - H281DRAFT_05721 0.85 -0.0 141,025 + -0.7 141,025 + +0.3 141,044 + +1.1 141,045 - +0.3 141,045 - -0.2 141,045 - +0.3 141,104 + +0.1 141,104 + -1.3 141,104 + -0.4 141,199 - H281DRAFT_05722 0.19 +0.7 141,199 - H281DRAFT_05722 0.19 -0.9 141,199 - H281DRAFT_05722 0.19 -0.6 141,295 - H281DRAFT_05722 0.31 +1.5 141,295 - H281DRAFT_05722 0.31 -0.2 141,295 - H281DRAFT_05722 0.31 +0.4 141,295 - H281DRAFT_05722 0.31 +1.4 141,295 - H281DRAFT_05722 0.31 +0.2 141,295 - H281DRAFT_05722 0.31 -0.3 141,295 - H281DRAFT_05722 0.31 -1.5 141,353 - H281DRAFT_05722 0.39 +1.0 141,527 - H281DRAFT_05722 0.61 +1.3 141,791 - -1.1 141,830 + +0.2 141,880 + +1.1 141,880 + +0.4 141,880 + -0.0 141,881 - +0.8 141,884 + +0.8 141,884 + +0.0 141,884 + +0.4 141,884 + -0.1 141,885 - -0.8 141,885 - +0.5 141,885 - +0.6 141,907 - +0.1 142,276 + H281DRAFT_05723 0.32 -0.4 142,276 + H281DRAFT_05723 0.32 +1.3 142,353 + H281DRAFT_05723 0.38 +0.4 142,354 - H281DRAFT_05723 0.38 -0.2 142,374 + H281DRAFT_05723 0.40 +0.5 142,374 + H281DRAFT_05723 0.40 -0.1 142,374 + H281DRAFT_05723 0.40 +0.4 142,375 - H281DRAFT_05723 0.40 +0.4 142,375 - H281DRAFT_05723 0.40 -0.1 142,375 - H281DRAFT_05723 0.40 -1.4 142,375 - H281DRAFT_05723 0.40 +1.1 142,375 - H281DRAFT_05723 0.40 -0.2 142,837 - H281DRAFT_05723 0.79 -1.0 142,933 + H281DRAFT_05723 0.87 -0.5 142,933 + H281DRAFT_05723 0.87 +0.4 142,934 - H281DRAFT_05723 0.87 -0.5 142,934 - H281DRAFT_05723 0.87 -0.0 142,934 - H281DRAFT_05723 0.87 +0.9 142,974 - -0.1 143,078 + -0.7 143,078 + +1.0 143,080 + -0.9 143,081 - +1.3 143,094 - +0.7 143,094 - +2.8 143,105 + +0.3 143,144 + +1.3 143,144 + +1.1 143,145 - +0.2 143,145 - +0.1 143,145 - +0.2 143,145 - -0.7 143,145 - +0.6 143,145 - -0.5 143,145 - -0.5 143,145 - +0.2 143,145 - +0.4 143,184 + +0.6 143,185 - +0.0 143,185 - +0.1 143,207 + -0.2 143,207 + +0.8 143,225 - -0.2 143,401 + H281DRAFT_05724 0.11 +0.5 143,867 + H281DRAFT_05724 0.38 +1.0 143,867 + H281DRAFT_05724 0.38 +0.7 143,867 + H281DRAFT_05724 0.38 +2.8 143,867 + H281DRAFT_05724 0.38 +0.8 143,867 + H281DRAFT_05724 0.38 +0.7 143,996 + H281DRAFT_05724 0.46 +0.2 143,996 + H281DRAFT_05724 0.46 +0.2
Or see this region's nucleotide sequence