Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05429

Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05428 and H281DRAFT_05429 are separated by 33 nucleotidesH281DRAFT_05429 and H281DRAFT_05430 are separated by 25 nucleotidesH281DRAFT_05430 and H281DRAFT_05431 overlap by 4 nucleotides H281DRAFT_05428: H281DRAFT_05428 - amino acid/amide ABC transporter substrate-binding protein, HAAT family, at 3,494 to 4,669 _05428 H281DRAFT_05429: H281DRAFT_05429 - Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit, at 4,703 to 6,001 _05429 H281DRAFT_05430: H281DRAFT_05430 - amino acid/amide ABC transporter ATP-binding protein 2, HAAT family, at 6,027 to 6,734 _05430 H281DRAFT_05431: H281DRAFT_05431 - amino acid/amide ABC transporter membrane protein 2, HAAT family /amino acid/amide ABC transporter ATP-binding protein 1, HAAT family, at 6,731 to 8,593 _05431 Position (kb) 4 5 6 7Strain fitness (log2 ratio) -2 -1 0 1at 3.764 kb on + strand, within H281DRAFT_05428at 3.774 kb on + strand, within H281DRAFT_05428at 3.774 kb on + strand, within H281DRAFT_05428at 3.775 kb on - strand, within H281DRAFT_05428at 3.873 kb on + strand, within H281DRAFT_05428at 3.873 kb on + strand, within H281DRAFT_05428at 3.873 kb on + strand, within H281DRAFT_05428at 3.874 kb on - strand, within H281DRAFT_05428at 3.874 kb on - strand, within H281DRAFT_05428at 3.990 kb on + strand, within H281DRAFT_05428at 3.991 kb on - strand, within H281DRAFT_05428at 4.114 kb on - strand, within H281DRAFT_05428at 4.186 kb on - strand, within H281DRAFT_05428at 4.186 kb on - strand, within H281DRAFT_05428at 4.191 kb on + strand, within H281DRAFT_05428at 4.206 kb on - strand, within H281DRAFT_05428at 4.239 kb on + strand, within H281DRAFT_05428at 4.284 kb on + strand, within H281DRAFT_05428at 4.285 kb on - strand, within H281DRAFT_05428at 4.302 kb on + strand, within H281DRAFT_05428at 4.317 kb on + strand, within H281DRAFT_05428at 4.329 kb on + strand, within H281DRAFT_05428at 4.330 kb on - strand, within H281DRAFT_05428at 4.480 kb on - strand, within H281DRAFT_05428at 4.675 kb on - strandat 4.704 kb on + strandat 4.835 kb on + strand, within H281DRAFT_05429at 4.879 kb on + strand, within H281DRAFT_05429at 4.952 kb on + strand, within H281DRAFT_05429at 4.952 kb on + strand, within H281DRAFT_05429at 4.953 kb on - strand, within H281DRAFT_05429at 4.953 kb on - strand, within H281DRAFT_05429at 4.953 kb on - strand, within H281DRAFT_05429at 5.022 kb on + strand, within H281DRAFT_05429at 5.023 kb on - strand, within H281DRAFT_05429at 5.100 kb on + strand, within H281DRAFT_05429at 5.112 kb on - strand, within H281DRAFT_05429at 5.177 kb on - strand, within H281DRAFT_05429at 5.179 kb on + strand, within H281DRAFT_05429at 5.179 kb on + strand, within H281DRAFT_05429at 5.180 kb on - strand, within H281DRAFT_05429at 5.180 kb on - strand, within H281DRAFT_05429at 5.218 kb on + strand, within H281DRAFT_05429at 5.219 kb on - strand, within H281DRAFT_05429at 5.358 kb on + strand, within H281DRAFT_05429at 5.358 kb on + strand, within H281DRAFT_05429at 5.358 kb on + strand, within H281DRAFT_05429at 5.396 kb on - strand, within H281DRAFT_05429at 5.627 kb on - strand, within H281DRAFT_05429at 5.629 kb on - strand, within H281DRAFT_05429at 5.719 kb on - strand, within H281DRAFT_05429at 5.982 kb on + strandat 6.001 kb on + strandat 6.069 kb on - strandat 6.340 kb on + strand, within H281DRAFT_05430at 6.340 kb on + strand, within H281DRAFT_05430at 6.432 kb on + strand, within H281DRAFT_05430at 6.433 kb on - strand, within H281DRAFT_05430at 6.691 kb on + strandat 6.691 kb on + strandat 6.692 kb on - strandat 6.793 kb on - strandat 6.802 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
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3,764 + H281DRAFT_05428 0.23 +0.1
3,774 + H281DRAFT_05428 0.24 +0.8
3,774 + H281DRAFT_05428 0.24 +0.6
3,775 - H281DRAFT_05428 0.24 -0.1
3,873 + H281DRAFT_05428 0.32 +0.1
3,873 + H281DRAFT_05428 0.32 -0.1
3,873 + H281DRAFT_05428 0.32 +0.3
3,874 - H281DRAFT_05428 0.32 +0.1
3,874 - H281DRAFT_05428 0.32 -0.5
3,990 + H281DRAFT_05428 0.42 -0.3
3,991 - H281DRAFT_05428 0.42 -1.1
4,114 - H281DRAFT_05428 0.53 +0.1
4,186 - H281DRAFT_05428 0.59 +0.7
4,186 - H281DRAFT_05428 0.59 +0.3
4,191 + H281DRAFT_05428 0.59 +0.2
4,206 - H281DRAFT_05428 0.61 -0.2
4,239 + H281DRAFT_05428 0.63 +0.7
4,284 + H281DRAFT_05428 0.67 +0.0
4,285 - H281DRAFT_05428 0.67 +0.8
4,302 + H281DRAFT_05428 0.69 -1.4
4,317 + H281DRAFT_05428 0.70 +0.4
4,329 + H281DRAFT_05428 0.71 +0.4
4,330 - H281DRAFT_05428 0.71 -0.6
4,480 - H281DRAFT_05428 0.84 -2.3
4,675 - -0.4
4,704 + -0.4
4,835 + H281DRAFT_05429 0.10 +0.8
4,879 + H281DRAFT_05429 0.14 -0.5
4,952 + H281DRAFT_05429 0.19 -0.4
4,952 + H281DRAFT_05429 0.19 +0.5
4,953 - H281DRAFT_05429 0.19 -0.3
4,953 - H281DRAFT_05429 0.19 +0.6
4,953 - H281DRAFT_05429 0.19 +0.3
5,022 + H281DRAFT_05429 0.25 +0.2
5,023 - H281DRAFT_05429 0.25 +0.2
5,100 + H281DRAFT_05429 0.31 -0.3
5,112 - H281DRAFT_05429 0.31 +0.2
5,177 - H281DRAFT_05429 0.36 +1.3
5,179 + H281DRAFT_05429 0.37 -0.1
5,179 + H281DRAFT_05429 0.37 +0.1
5,180 - H281DRAFT_05429 0.37 +0.5
5,180 - H281DRAFT_05429 0.37 +0.3
5,218 + H281DRAFT_05429 0.40 +1.6
5,219 - H281DRAFT_05429 0.40 -0.5
5,358 + H281DRAFT_05429 0.50 +0.0
5,358 + H281DRAFT_05429 0.50 -2.3
5,358 + H281DRAFT_05429 0.50 -0.6
5,396 - H281DRAFT_05429 0.53 +0.7
5,627 - H281DRAFT_05429 0.71 +0.1
5,629 - H281DRAFT_05429 0.71 -0.7
5,719 - H281DRAFT_05429 0.78 -0.2
5,982 + +0.4
6,001 + +0.3
6,069 - -0.1
6,340 + H281DRAFT_05430 0.44 -0.4
6,340 + H281DRAFT_05430 0.44 -0.3
6,432 + H281DRAFT_05430 0.57 +0.2
6,433 - H281DRAFT_05430 0.57 -1.6
6,691 + +0.4
6,691 + +1.2
6,692 - -0.5
6,793 - +0.4
6,802 + -0.3

Or see this region's nucleotide sequence