Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04080 and H281DRAFT_04081 are separated by 14 nucleotides H281DRAFT_04081 and H281DRAFT_04082 are separated by 75 nucleotides H281DRAFT_04082 and H281DRAFT_04083 overlap by 4 nucleotides H281DRAFT_04083 and H281DRAFT_04084 are separated by 549 nucleotides
H281DRAFT_04080: H281DRAFT_04080 - luciferase family oxidoreductase, group 1, at 184,076 to 185,095
_04080
H281DRAFT_04081: H281DRAFT_04081 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 185,110 to 185,556
_04081
H281DRAFT_04082: H281DRAFT_04082 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 185,632 to 187,929
_04082
H281DRAFT_04083: H281DRAFT_04083 - ATP-dependent Clp protease adaptor protein ClpS, at 187,926 to 188,240
_04083
H281DRAFT_04084: H281DRAFT_04084 - cold-shock DNA-binding protein family, at 188,790 to 188,996
_04084
Position (kb) 185
186
187
188 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 184.730 kb on + strand, within H281DRAFT_04080 at 184.827 kb on - strand, within H281DRAFT_04080 at 184.877 kb on + strand, within H281DRAFT_04080 at 184.924 kb on + strand, within H281DRAFT_04080 at 184.924 kb on + strand, within H281DRAFT_04080 at 184.925 kb on - strand at 184.968 kb on + strand, within H281DRAFT_04080 at 185.022 kb on - strand at 185.043 kb on - strand at 185.043 kb on - strand at 185.620 kb on + strand at 185.634 kb on - strand at 186.201 kb on - strand, within H281DRAFT_04082 at 186.201 kb on - strand, within H281DRAFT_04082 at 186.350 kb on + strand, within H281DRAFT_04082 at 186.351 kb on - strand, within H281DRAFT_04082 at 186.351 kb on - strand, within H281DRAFT_04082 at 186.351 kb on - strand, within H281DRAFT_04082 at 187.146 kb on - strand, within H281DRAFT_04082 at 187.285 kb on + strand, within H281DRAFT_04082 at 187.559 kb on + strand, within H281DRAFT_04082 at 187.559 kb on + strand, within H281DRAFT_04082 at 187.562 kb on + strand, within H281DRAFT_04082 at 187.563 kb on - strand, within H281DRAFT_04082 at 188.018 kb on + strand, within H281DRAFT_04083 at 188.021 kb on - strand, within H281DRAFT_04083 at 188.021 kb on - strand, within H281DRAFT_04083 at 188.021 kb on - strand, within H281DRAFT_04083 at 188.063 kb on + strand, within H281DRAFT_04083 at 188.064 kb on - strand, within H281DRAFT_04083 at 188.064 kb on - strand, within H281DRAFT_04083 at 188.064 kb on - strand at 188.099 kb on - strand, within H281DRAFT_04083 at 188.099 kb on - strand, within H281DRAFT_04083 at 188.164 kb on + strand, within H281DRAFT_04083 at 188.164 kb on + strand, within H281DRAFT_04083 at 188.165 kb on - strand, within H281DRAFT_04083 at 188.165 kb on - strand, within H281DRAFT_04083 at 188.205 kb on + strand, within H281DRAFT_04083 at 188.206 kb on - strand, within H281DRAFT_04083 at 188.232 kb on - strand at 188.240 kb on + strand at 188.240 kb on + strand at 188.240 kb on + strand at 188.251 kb on + strand at 188.252 kb on - strand at 188.252 kb on - strand at 188.259 kb on + strand at 188.259 kb on + strand at 188.259 kb on + strand at 188.259 kb on + strand at 188.260 kb on - strand at 188.383 kb on - strand at 188.556 kb on - strand at 188.556 kb on - strand at 188.556 kb on - strand at 188.603 kb on - strand at 188.636 kb on - strand at 188.774 kb on + strand at 188.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C) remove 184,730 + H281DRAFT_04080 0.64 +0.1 184,827 - H281DRAFT_04080 0.74 +0.3 184,877 + H281DRAFT_04080 0.79 -0.3 184,924 + H281DRAFT_04080 0.83 +0.3 184,924 + H281DRAFT_04080 0.83 -0.7 184,925 - +2.6 184,968 + H281DRAFT_04080 0.87 +0.3 185,022 - +3.7 185,043 - +1.3 185,043 - +0.1 185,620 + +0.7 185,634 - +0.1 186,201 - H281DRAFT_04082 0.25 -2.4 186,201 - H281DRAFT_04082 0.25 -1.3 186,350 + H281DRAFT_04082 0.31 -0.7 186,351 - H281DRAFT_04082 0.31 -2.0 186,351 - H281DRAFT_04082 0.31 -1.0 186,351 - H281DRAFT_04082 0.31 -2.7 187,146 - H281DRAFT_04082 0.66 -0.6 187,285 + H281DRAFT_04082 0.72 -3.9 187,559 + H281DRAFT_04082 0.84 -2.6 187,559 + H281DRAFT_04082 0.84 -2.8 187,562 + H281DRAFT_04082 0.84 -2.0 187,563 - H281DRAFT_04082 0.84 -1.6 188,018 + H281DRAFT_04083 0.29 -2.1 188,021 - H281DRAFT_04083 0.30 +0.0 188,021 - H281DRAFT_04083 0.30 -0.8 188,021 - H281DRAFT_04083 0.30 +0.1 188,063 + H281DRAFT_04083 0.43 -1.9 188,064 - H281DRAFT_04083 0.44 +0.2 188,064 - H281DRAFT_04083 0.44 -0.1 188,064 - -1.3 188,099 - H281DRAFT_04083 0.55 -0.7 188,099 - H281DRAFT_04083 0.55 +0.4 188,164 + H281DRAFT_04083 0.76 -1.4 188,164 + H281DRAFT_04083 0.76 -1.4 188,165 - H281DRAFT_04083 0.76 -0.5 188,165 - H281DRAFT_04083 0.76 -0.4 188,205 + H281DRAFT_04083 0.89 +0.4 188,206 - H281DRAFT_04083 0.89 -2.4 188,232 - -0.7 188,240 + -0.2 188,240 + -0.9 188,240 + -1.2 188,251 + -1.2 188,252 - -0.9 188,252 - -0.3 188,259 + -3.7 188,259 + -4.7 188,259 + -2.2 188,259 + -2.6 188,260 - -0.3 188,383 - +0.3 188,556 - -0.4 188,556 - -0.5 188,556 - -0.3 188,603 - -0.7 188,636 - -1.5 188,774 + -0.5 188,775 - -0.7
Or see this region's nucleotide sequence