Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_03305

Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_03303 and H281DRAFT_03304 are separated by 93 nucleotidesH281DRAFT_03304 and H281DRAFT_03305 are separated by 282 nucleotidesH281DRAFT_03305 and H281DRAFT_03306 are separated by 31 nucleotides H281DRAFT_03303: H281DRAFT_03303 - NAD+ synthase (glutamine-hydrolysing), at 365,314 to 367,362 _03303 H281DRAFT_03304: H281DRAFT_03304 - amidohydrolase, PncC family, at 367,456 to 367,989 _03304 H281DRAFT_03305: H281DRAFT_03305 - Acetyltransferases, at 368,272 to 368,781 _03305 H281DRAFT_03306: H281DRAFT_03306 - Cytochrome c, mono- and diheme variants, at 368,813 to 370,117 _03306 Position (kb) 368 369Strain fitness (log2 ratio) -1 0 1 2at 367.739 kb on - strand, within H281DRAFT_03304at 368.588 kb on + strandat 368.589 kb on - strand, within H281DRAFT_03305at 368.690 kb on - strand, within H281DRAFT_03305at 368.690 kb on - strand, within H281DRAFT_03305at 368.690 kb on - strand, within H281DRAFT_03305at 369.069 kb on - strand, within H281DRAFT_03306at 369.718 kb on - strand, within H281DRAFT_03306

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
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367,739 - H281DRAFT_03304 0.53 -0.8
368,588 + +2.5
368,589 - H281DRAFT_03305 0.62 +0.7
368,690 - H281DRAFT_03305 0.82 +0.1
368,690 - H281DRAFT_03305 0.82 -0.5
368,690 - H281DRAFT_03305 0.82 +0.7
369,069 - H281DRAFT_03306 0.20 -1.0
369,718 - H281DRAFT_03306 0.69 -0.7

Or see this region's nucleotide sequence