Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_03303

Experiment: 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_03301 and H281DRAFT_03302 are separated by 57 nucleotidesH281DRAFT_03302 and H281DRAFT_03303 are separated by 148 nucleotidesH281DRAFT_03303 and H281DRAFT_03304 are separated by 93 nucleotidesH281DRAFT_03304 and H281DRAFT_03305 are separated by 282 nucleotides H281DRAFT_03301: H281DRAFT_03301 - oxidoreductase alpha (molybdopterin) subunit, at 362,214 to 364,520 _03301 H281DRAFT_03302: H281DRAFT_03302 - Hemerythrin HHE cation binding domain-containing protein, at 364,578 to 365,165 _03302 H281DRAFT_03303: H281DRAFT_03303 - NAD+ synthase (glutamine-hydrolysing), at 365,314 to 367,362 _03303 H281DRAFT_03304: H281DRAFT_03304 - amidohydrolase, PncC family, at 367,456 to 367,989 _03304 H281DRAFT_03305: H281DRAFT_03305 - Acetyltransferases, at 368,272 to 368,781 _03305 Position (kb) 365 366 367 368Strain fitness (log2 ratio) -1 0 1 2 3at 364.673 kb on + strand, within H281DRAFT_03302at 364.674 kb on - strand, within H281DRAFT_03302at 364.682 kb on + strand, within H281DRAFT_03302at 365.106 kb on - strand, within H281DRAFT_03302at 365.513 kb on + strandat 365.628 kb on - strand, within H281DRAFT_03303at 365.628 kb on - strand, within H281DRAFT_03303at 366.172 kb on - strand, within H281DRAFT_03303at 366.320 kb on + strand, within H281DRAFT_03303at 367.182 kb on + strandat 367.739 kb on - strand, within H281DRAFT_03304

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 2.5 mM (C)
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364,673 + H281DRAFT_03302 0.16 +0.2
364,674 - H281DRAFT_03302 0.16 -0.4
364,682 + H281DRAFT_03302 0.18 +0.0
365,106 - H281DRAFT_03302 0.90 -0.5
365,513 + +0.7
365,628 - H281DRAFT_03303 0.15 +3.4
365,628 - H281DRAFT_03303 0.15 +1.0
366,172 - H281DRAFT_03303 0.42 +1.2
366,320 + H281DRAFT_03303 0.49 +1.4
367,182 + -0.2
367,739 - H281DRAFT_03304 0.53 -0.8

Or see this region's nucleotide sequence