Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3211 and Dsui_3212 are separated by 69 nucleotides Dsui_3212 and Dsui_3213 are separated by 330 nucleotides Dsui_3213 and Dsui_3214 are separated by 125 nucleotides
Dsui_3211: Dsui_3211 - glutamate dehydrogenase/leucine dehydrogenase, at 3,445,273 to 3,446,619
_3211
Dsui_3212: Dsui_3212 - acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, at 3,446,689 to 3,448,356
_3212
Dsui_3213: Dsui_3213 - CBS domain-containing protein, at 3,448,687 to 3,449,136
_3213
Dsui_3214: Dsui_3214 - ATP-dependent exonuclase V beta subunit, helicase and exonuclease domain-containing, at 3,449,262 to 3,452,603
_3214
Position (kb)
3446
3447
3448
3449 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3445.797 kb on - strand at 3445.889 kb on - strand at 3445.983 kb on - strand, within Dsui_3211 at 3446.075 kb on + strand, within Dsui_3211 at 3446.206 kb on - strand, within Dsui_3211 at 3446.261 kb on + strand, within Dsui_3211 at 3446.329 kb on - strand, within Dsui_3211 at 3446.384 kb on + strand, within Dsui_3211 at 3446.429 kb on + strand, within Dsui_3211 at 3446.464 kb on - strand, within Dsui_3211 at 3446.775 kb on + strand at 3446.798 kb on - strand at 3446.807 kb on + strand at 3446.817 kb on + strand at 3446.837 kb on + strand at 3446.892 kb on - strand, within Dsui_3212 at 3446.979 kb on - strand, within Dsui_3212 at 3447.062 kb on + strand at 3447.070 kb on - strand, within Dsui_3212 at 3447.096 kb on + strand, within Dsui_3212 at 3447.142 kb on + strand, within Dsui_3212 at 3447.171 kb on + strand at 3447.237 kb on + strand, within Dsui_3212 at 3447.245 kb on + strand, within Dsui_3212 at 3447.246 kb on + strand, within Dsui_3212 at 3447.358 kb on + strand at 3447.366 kb on - strand, within Dsui_3212 at 3447.366 kb on - strand, within Dsui_3212 at 3447.433 kb on + strand at 3447.438 kb on + strand, within Dsui_3212 at 3447.474 kb on - strand, within Dsui_3212 at 3447.486 kb on - strand, within Dsui_3212 at 3447.540 kb on + strand, within Dsui_3212 at 3447.549 kb on - strand, within Dsui_3212 at 3447.559 kb on - strand, within Dsui_3212 at 3447.561 kb on + strand, within Dsui_3212 at 3447.634 kb on + strand at 3447.637 kb on - strand, within Dsui_3212 at 3447.668 kb on + strand, within Dsui_3212 at 3447.770 kb on + strand at 3447.783 kb on - strand, within Dsui_3212 at 3447.799 kb on - strand, within Dsui_3212 at 3447.852 kb on + strand, within Dsui_3212 at 3447.860 kb on - strand, within Dsui_3212 at 3447.937 kb on + strand, within Dsui_3212 at 3447.945 kb on - strand, within Dsui_3212 at 3447.948 kb on + strand, within Dsui_3212 at 3447.956 kb on - strand, within Dsui_3212 at 3448.039 kb on - strand, within Dsui_3212 at 3448.056 kb on - strand, within Dsui_3212 at 3448.061 kb on - strand, within Dsui_3212 at 3448.084 kb on - strand, within Dsui_3212 at 3448.102 kb on - strand, within Dsui_3212 at 3448.120 kb on - strand, within Dsui_3212 at 3448.144 kb on - strand, within Dsui_3212 at 3448.202 kb on + strand at 3448.252 kb on - strand at 3448.254 kb on + strand at 3448.256 kb on + strand at 3448.259 kb on - strand at 3448.264 kb on - strand at 3448.366 kb on - strand at 3448.421 kb on - strand at 3448.472 kb on + strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.603 kb on - strand at 3448.690 kb on + strand at 3448.809 kb on - strand, within Dsui_3213 at 3448.900 kb on - strand, within Dsui_3213 at 3449.065 kb on + strand at 3449.065 kb on + strand at 3449.087 kb on + strand at 3449.095 kb on - strand at 3449.095 kb on - strand at 3449.095 kb on - strand at 3449.095 kb on - strand at 3449.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 3,445,797 - -1.0 3,445,889 - -2.2 3,445,983 - Dsui_3211 0.53 -2.7 3,446,075 + Dsui_3211 0.60 +0.1 3,446,206 - Dsui_3211 0.69 -1.0 3,446,261 + Dsui_3211 0.73 -0.9 3,446,329 - Dsui_3211 0.78 -0.3 3,446,384 + Dsui_3211 0.82 -0.3 3,446,429 + Dsui_3211 0.86 -0.5 3,446,464 - Dsui_3211 0.88 -1.1 3,446,775 + -0.3 3,446,798 - -2.0 3,446,807 + +2.3 3,446,817 + -0.2 3,446,837 + +0.2 3,446,892 - Dsui_3212 0.12 +0.6 3,446,979 - Dsui_3212 0.17 -2.4 3,447,062 + -1.0 3,447,070 - Dsui_3212 0.23 +0.7 3,447,096 + Dsui_3212 0.24 -2.4 3,447,142 + Dsui_3212 0.27 +0.1 3,447,171 + +2.3 3,447,237 + Dsui_3212 0.33 -3.3 3,447,245 + Dsui_3212 0.33 -2.9 3,447,246 + Dsui_3212 0.33 -0.0 3,447,358 + -1.0 3,447,366 - Dsui_3212 0.41 -0.0 3,447,366 - Dsui_3212 0.41 +1.6 3,447,433 + -3.2 3,447,438 + Dsui_3212 0.45 -3.9 3,447,474 - Dsui_3212 0.47 +0.7 3,447,486 - Dsui_3212 0.48 -0.1 3,447,540 + Dsui_3212 0.51 -2.9 3,447,549 - Dsui_3212 0.52 -1.7 3,447,559 - Dsui_3212 0.52 +0.0 3,447,561 + Dsui_3212 0.52 -1.0 3,447,634 + +0.5 3,447,637 - Dsui_3212 0.57 +1.5 3,447,668 + Dsui_3212 0.59 -1.2 3,447,770 + +1.8 3,447,783 - Dsui_3212 0.66 -1.8 3,447,799 - Dsui_3212 0.67 -0.1 3,447,852 + Dsui_3212 0.70 -0.9 3,447,860 - Dsui_3212 0.70 -0.0 3,447,937 + Dsui_3212 0.75 -0.7 3,447,945 - Dsui_3212 0.75 -0.0 3,447,948 + Dsui_3212 0.75 -0.9 3,447,956 - Dsui_3212 0.76 +1.1 3,448,039 - Dsui_3212 0.81 +0.3 3,448,056 - Dsui_3212 0.82 +0.2 3,448,061 - Dsui_3212 0.82 +0.6 3,448,084 - Dsui_3212 0.84 +0.4 3,448,102 - Dsui_3212 0.85 -0.3 3,448,120 - Dsui_3212 0.86 +0.4 3,448,144 - Dsui_3212 0.87 -0.7 3,448,202 + -0.3 3,448,252 - -0.1 3,448,254 + -1.7 3,448,256 + -1.2 3,448,259 - -0.5 3,448,264 - +1.6 3,448,366 - +0.9 3,448,421 - +0.3 3,448,472 + -0.5 3,448,480 - -0.1 3,448,480 - +1.5 3,448,480 - -2.0 3,448,480 - +0.3 3,448,480 - -2.5 3,448,603 - +0.2 3,448,690 + +0.2 3,448,809 - Dsui_3213 0.27 +0.3 3,448,900 - Dsui_3213 0.47 +1.2 3,449,065 + +0.8 3,449,065 + -0.8 3,449,087 + -2.0 3,449,095 - +1.3 3,449,095 - +1.1 3,449,095 - +0.1 3,449,095 - +2.2 3,449,183 - +1.0
Or see this region's nucleotide sequence