Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2299 and Dsui_2300 are separated by 57 nucleotides Dsui_2300 and Dsui_2301 are separated by 180 nucleotides Dsui_2301 and Dsui_2302 are separated by 73 nucleotides
Dsui_2299: Dsui_2299 - flavin-dependent dehydrogenase, at 2,453,467 to 2,455,113
_2299
Dsui_2300: Dsui_2300 - hypothetical protein, at 2,455,171 to 2,455,863
_2300
Dsui_2301: Dsui_2301 - diguanylate cyclase (GGDEF) domain-containing protein, at 2,456,044 to 2,458,458
_2301
Dsui_2302: Dsui_2302 - acyl-CoA dehydrogenase, at 2,458,532 to 2,460,325
_2302
Position (kb)
2456
2457
2458
2459 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2455.179 kb on - strand at 2455.184 kb on + strand at 2455.192 kb on - strand at 2455.210 kb on + strand at 2455.210 kb on + strand at 2455.238 kb on - strand at 2455.303 kb on + strand, within Dsui_2300 at 2455.370 kb on + strand at 2455.437 kb on + strand, within Dsui_2300 at 2455.464 kb on + strand, within Dsui_2300 at 2455.464 kb on + strand, within Dsui_2300 at 2455.590 kb on - strand, within Dsui_2300 at 2455.604 kb on + strand, within Dsui_2300 at 2455.647 kb on - strand, within Dsui_2300 at 2455.666 kb on + strand, within Dsui_2300 at 2455.666 kb on - strand, within Dsui_2300 at 2455.721 kb on - strand at 2455.772 kb on - strand, within Dsui_2300 at 2455.773 kb on - strand at 2455.848 kb on + strand at 2455.901 kb on + strand at 2455.901 kb on + strand at 2455.911 kb on + strand at 2455.919 kb on - strand at 2455.919 kb on - strand at 2456.015 kb on + strand at 2456.243 kb on + strand at 2456.420 kb on + strand, within Dsui_2301 at 2456.439 kb on - strand at 2456.579 kb on - strand, within Dsui_2301 at 2456.623 kb on + strand at 2456.631 kb on + strand, within Dsui_2301 at 2456.637 kb on - strand, within Dsui_2301 at 2456.787 kb on + strand, within Dsui_2301 at 2456.872 kb on + strand, within Dsui_2301 at 2456.933 kb on + strand, within Dsui_2301 at 2456.974 kb on + strand, within Dsui_2301 at 2456.974 kb on + strand, within Dsui_2301 at 2456.986 kb on + strand, within Dsui_2301 at 2457.178 kb on + strand, within Dsui_2301 at 2457.330 kb on - strand, within Dsui_2301 at 2457.385 kb on - strand at 2457.413 kb on - strand, within Dsui_2301 at 2457.490 kb on + strand, within Dsui_2301 at 2457.531 kb on + strand, within Dsui_2301 at 2457.531 kb on + strand, within Dsui_2301 at 2457.531 kb on + strand, within Dsui_2301 at 2457.578 kb on - strand at 2457.725 kb on + strand at 2457.755 kb on + strand, within Dsui_2301 at 2457.765 kb on + strand, within Dsui_2301 at 2457.768 kb on - strand, within Dsui_2301 at 2457.772 kb on + strand, within Dsui_2301 at 2457.794 kb on - strand, within Dsui_2301 at 2457.829 kb on + strand, within Dsui_2301 at 2457.836 kb on + strand at 2457.975 kb on + strand at 2457.983 kb on - strand, within Dsui_2301 at 2458.022 kb on + strand, within Dsui_2301 at 2458.022 kb on + strand, within Dsui_2301 at 2458.029 kb on - strand, within Dsui_2301 at 2458.113 kb on + strand, within Dsui_2301 at 2458.157 kb on + strand, within Dsui_2301 at 2458.157 kb on + strand, within Dsui_2301 at 2458.178 kb on - strand, within Dsui_2301 at 2458.209 kb on + strand at 2458.236 kb on - strand at 2458.346 kb on - strand at 2458.437 kb on - strand at 2458.499 kb on - strand at 2458.504 kb on - strand at 2458.623 kb on + strand at 2458.779 kb on + strand, within Dsui_2302 at 2458.787 kb on - strand, within Dsui_2302 at 2459.119 kb on + strand, within Dsui_2302 at 2459.132 kb on - strand, within Dsui_2302 at 2459.135 kb on - strand, within Dsui_2302 at 2459.168 kb on - strand, within Dsui_2302 at 2459.245 kb on - strand, within Dsui_2302 at 2459.383 kb on + strand, within Dsui_2302 at 2459.412 kb on - strand, within Dsui_2302
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,455,179 - -2.1 2,455,184 + +1.1 2,455,192 - -2.5 2,455,210 + -1.4 2,455,210 + -0.2 2,455,238 - -1.9 2,455,303 + Dsui_2300 0.19 -1.3 2,455,370 + -0.7 2,455,437 + Dsui_2300 0.38 +1.0 2,455,464 + Dsui_2300 0.42 +1.3 2,455,464 + Dsui_2300 0.42 +0.9 2,455,590 - Dsui_2300 0.60 -1.3 2,455,604 + Dsui_2300 0.62 -0.3 2,455,647 - Dsui_2300 0.69 -2.0 2,455,666 + Dsui_2300 0.71 -0.9 2,455,666 - Dsui_2300 0.71 -1.7 2,455,721 - +0.3 2,455,772 - Dsui_2300 0.87 -2.3 2,455,773 - -2.3 2,455,848 + +2.1 2,455,901 + +0.6 2,455,901 + -1.9 2,455,911 + -1.3 2,455,919 - -2.8 2,455,919 - -2.1 2,456,015 + -1.3 2,456,243 + -0.3 2,456,420 + Dsui_2301 0.16 -1.7 2,456,439 - -0.7 2,456,579 - Dsui_2301 0.22 -3.3 2,456,623 + -2.3 2,456,631 + Dsui_2301 0.24 -2.7 2,456,637 - Dsui_2301 0.25 -0.3 2,456,787 + Dsui_2301 0.31 -0.3 2,456,872 + Dsui_2301 0.34 -0.1 2,456,933 + Dsui_2301 0.37 -0.0 2,456,974 + Dsui_2301 0.39 +1.6 2,456,974 + Dsui_2301 0.39 -4.1 2,456,986 + Dsui_2301 0.39 -1.8 2,457,178 + Dsui_2301 0.47 -3.7 2,457,330 - Dsui_2301 0.53 +0.2 2,457,385 - -0.7 2,457,413 - Dsui_2301 0.57 -0.6 2,457,490 + Dsui_2301 0.60 -2.9 2,457,531 + Dsui_2301 0.62 -2.9 2,457,531 + Dsui_2301 0.62 -0.7 2,457,531 + Dsui_2301 0.62 +1.6 2,457,578 - +0.5 2,457,725 + +0.7 2,457,755 + Dsui_2301 0.71 -4.7 2,457,765 + Dsui_2301 0.71 -2.9 2,457,768 - Dsui_2301 0.71 -4.5 2,457,772 + Dsui_2301 0.72 -2.0 2,457,794 - Dsui_2301 0.72 -3.6 2,457,829 + Dsui_2301 0.74 -4.2 2,457,836 + -2.5 2,457,975 + -2.4 2,457,983 - Dsui_2301 0.80 +1.2 2,458,022 + Dsui_2301 0.82 -5.2 2,458,022 + Dsui_2301 0.82 -3.8 2,458,029 - Dsui_2301 0.82 +0.7 2,458,113 + Dsui_2301 0.86 -1.7 2,458,157 + Dsui_2301 0.87 -1.6 2,458,157 + Dsui_2301 0.87 -0.6 2,458,178 - Dsui_2301 0.88 +0.3 2,458,209 + -1.0 2,458,236 - -2.6 2,458,346 - +0.2 2,458,437 - +1.3 2,458,499 - +0.9 2,458,504 - +0.1 2,458,623 + +2.3 2,458,779 + Dsui_2302 0.14 -1.7 2,458,787 - Dsui_2302 0.14 -0.6 2,459,119 + Dsui_2302 0.33 -3.0 2,459,132 - Dsui_2302 0.33 -1.1 2,459,135 - Dsui_2302 0.34 -1.1 2,459,168 - Dsui_2302 0.35 +1.7 2,459,245 - Dsui_2302 0.40 -1.2 2,459,383 + Dsui_2302 0.47 -0.7 2,459,412 - Dsui_2302 0.49 -0.1
Or see this region's nucleotide sequence