Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2187 and Dsui_2188 are separated by 320 nucleotides Dsui_2188 and Dsui_2189 are separated by 145 nucleotides Dsui_2189 and Dsui_2190 are separated by 316 nucleotides Dsui_2190 and Dsui_2191 are separated by 10 nucleotides
Dsui_2187: Dsui_2187 - acyl-CoA synthetase/AMP-acid ligase, at 2,321,416 to 2,323,113
_2187
Dsui_2188: Dsui_2188 - Protein of unknown function (DUF3460), at 2,323,434 to 2,323,625
_2188
Dsui_2189: Dsui_2189 - acyl-CoA synthetase (NDP forming), at 2,323,771 to 2,326,461
_2189
Dsui_2190: Dsui_2190 - Tellurite resistance protein TehB, at 2,326,778 to 2,327,332
_2190
Dsui_2191: Dsui_2191 - squalene-associated FAD-dependent desaturase, at 2,327,343 to 2,328,680
_2191
Position (kb)
2323
2324
2325
2326
2327 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2322.782 kb on - strand at 2322.804 kb on + strand, within Dsui_2187 at 2322.850 kb on - strand, within Dsui_2187 at 2322.906 kb on - strand at 2323.017 kb on + strand at 2323.034 kb on + strand at 2323.048 kb on + strand at 2323.096 kb on + strand at 2323.176 kb on - strand at 2323.329 kb on + strand at 2323.431 kb on + strand at 2323.450 kb on - strand at 2323.457 kb on + strand, within Dsui_2188 at 2323.547 kb on + strand, within Dsui_2188 at 2323.587 kb on + strand, within Dsui_2188 at 2323.595 kb on + strand, within Dsui_2188 at 2323.619 kb on - strand at 2323.640 kb on + strand at 2323.669 kb on + strand at 2323.669 kb on - strand at 2323.749 kb on + strand at 2324.195 kb on - strand, within Dsui_2189 at 2324.280 kb on - strand, within Dsui_2189 at 2324.420 kb on + strand at 2324.474 kb on - strand, within Dsui_2189 at 2324.764 kb on - strand at 2324.974 kb on - strand, within Dsui_2189 at 2325.069 kb on + strand at 2325.199 kb on + strand at 2325.290 kb on + strand at 2325.736 kb on - strand, within Dsui_2189 at 2325.754 kb on - strand, within Dsui_2189 at 2325.827 kb on - strand, within Dsui_2189 at 2325.836 kb on - strand, within Dsui_2189 at 2325.847 kb on - strand, within Dsui_2189 at 2326.085 kb on - strand, within Dsui_2189 at 2326.087 kb on - strand, within Dsui_2189 at 2326.087 kb on - strand, within Dsui_2189 at 2326.222 kb on - strand at 2326.222 kb on - strand at 2326.456 kb on + strand at 2326.584 kb on + strand at 2326.773 kb on - strand at 2326.842 kb on - strand at 2326.842 kb on - strand at 2327.039 kb on - strand at 2327.071 kb on + strand at 2327.088 kb on + strand, within Dsui_2190 at 2327.111 kb on + strand, within Dsui_2190 at 2327.135 kb on - strand at 2327.294 kb on + strand at 2327.334 kb on - strand at 2327.334 kb on - strand at 2327.334 kb on - strand at 2327.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,322,782 - -1.4 2,322,804 + Dsui_2187 0.82 -1.4 2,322,850 - Dsui_2187 0.84 -1.7 2,322,906 - -2.0 2,323,017 + +0.5 2,323,034 + -0.9 2,323,048 + -0.3 2,323,096 + -2.2 2,323,176 - -1.2 2,323,329 + +1.2 2,323,431 + -0.3 2,323,450 - -1.8 2,323,457 + Dsui_2188 0.12 +1.4 2,323,547 + Dsui_2188 0.59 -0.5 2,323,587 + Dsui_2188 0.80 +0.5 2,323,595 + Dsui_2188 0.84 +1.3 2,323,619 - +0.4 2,323,640 + +0.3 2,323,669 + +1.4 2,323,669 - -1.5 2,323,749 + -3.2 2,324,195 - Dsui_2189 0.16 -4.1 2,324,280 - Dsui_2189 0.19 -3.3 2,324,420 + -1.8 2,324,474 - Dsui_2189 0.26 -3.8 2,324,764 - -2.9 2,324,974 - Dsui_2189 0.45 +0.2 2,325,069 + -2.6 2,325,199 + -3.3 2,325,290 + -3.2 2,325,736 - Dsui_2189 0.73 -2.7 2,325,754 - Dsui_2189 0.74 -2.2 2,325,827 - Dsui_2189 0.76 -3.5 2,325,836 - Dsui_2189 0.77 -4.4 2,325,847 - Dsui_2189 0.77 -3.7 2,326,085 - Dsui_2189 0.86 -3.2 2,326,087 - Dsui_2189 0.86 -3.5 2,326,087 - Dsui_2189 0.86 -3.0 2,326,222 - -3.9 2,326,222 - -4.1 2,326,456 + -1.9 2,326,584 + -0.5 2,326,773 - -2.5 2,326,842 - -2.3 2,326,842 - -2.8 2,327,039 - -0.4 2,327,071 + -0.1 2,327,088 + Dsui_2190 0.56 +2.0 2,327,111 + Dsui_2190 0.60 -2.1 2,327,135 - -2.3 2,327,294 + +0.1 2,327,334 - +0.2 2,327,334 - -0.6 2,327,334 - -0.2 2,327,359 - +0.3
Or see this region's nucleotide sequence