Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0469 and Dsui_0470 are separated by 31 nucleotides Dsui_0470 and Dsui_0471 overlap by 16 nucleotides Dsui_0471 and Dsui_0472 are separated by 10 nucleotides
Dsui_0469: Dsui_0469 - lipopolysaccharide heptosyltransferase III, putative, at 476,462 to 477,565
_0469
Dsui_0470: Dsui_0470 - fructose-2,6-bisphosphatase, at 477,597 to 478,166
_0470
Dsui_0471: Dsui_0471 - cobalamin 5''-phosphate synthase/cobalamin synthase, at 478,151 to 478,927
_0471
Dsui_0472: Dsui_0472 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 478,938 to 479,993
_0472
Position (kb) 478
479 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 477.165 kb on - strand, within Dsui_0469 at 477.463 kb on - strand at 477.688 kb on + strand, within Dsui_0470 at 477.696 kb on - strand, within Dsui_0470 at 477.696 kb on - strand, within Dsui_0470 at 477.798 kb on + strand at 477.918 kb on - strand, within Dsui_0470 at 477.995 kb on + strand, within Dsui_0470 at 478.134 kb on + strand at 478.169 kb on - strand at 478.169 kb on - strand at 478.199 kb on + strand at 478.325 kb on - strand, within Dsui_0471 at 478.341 kb on + strand at 478.442 kb on - strand, within Dsui_0471 at 478.583 kb on - strand, within Dsui_0471 at 478.720 kb on + strand at 478.737 kb on + strand, within Dsui_0471 at 478.943 kb on - strand at 478.943 kb on - strand at 478.992 kb on - strand at 479.080 kb on - strand, within Dsui_0472 at 479.138 kb on - strand, within Dsui_0472 at 479.251 kb on + strand at 479.283 kb on - strand, within Dsui_0472 at 479.335 kb on + strand at 479.343 kb on - strand, within Dsui_0472 at 479.582 kb on + strand, within Dsui_0472 at 479.678 kb on + strand at 479.678 kb on + strand, within Dsui_0472 at 479.752 kb on + strand, within Dsui_0472 at 479.891 kb on + strand at 479.908 kb on + strand at 479.908 kb on + strand at 479.908 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 477,165 - Dsui_0469 0.64 -3.9 477,463 - -1.8 477,688 + Dsui_0470 0.16 +0.6 477,696 - Dsui_0470 0.17 -0.7 477,696 - Dsui_0470 0.17 +0.8 477,798 + -3.2 477,918 - Dsui_0470 0.56 +0.4 477,995 + Dsui_0470 0.70 -3.2 478,134 + -2.7 478,169 - +0.4 478,169 - +0.4 478,199 + -1.2 478,325 - Dsui_0471 0.22 -0.4 478,341 + +0.3 478,442 - Dsui_0471 0.37 -0.4 478,583 - Dsui_0471 0.56 -0.3 478,720 + +0.1 478,737 + Dsui_0471 0.75 +0.2 478,943 - +0.3 478,943 - +0.6 478,992 - +1.5 479,080 - Dsui_0472 0.13 -0.4 479,138 - Dsui_0472 0.19 +0.5 479,251 + -1.9 479,283 - Dsui_0472 0.33 -0.7 479,335 + +1.1 479,343 - Dsui_0472 0.38 -0.5 479,582 + Dsui_0472 0.61 -0.3 479,678 + +0.1 479,678 + Dsui_0472 0.70 -2.8 479,752 + Dsui_0472 0.77 -2.5 479,891 + -1.8 479,908 + -2.8 479,908 + -0.4 479,908 + -0.3
Or see this region's nucleotide sequence