Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0003 and Dsui_0004 are separated by 42 nucleotides Dsui_0004 and Dsui_0005 are separated by 32 nucleotides Dsui_0005 and Dsui_0006 are separated by 9 nucleotides Dsui_0006 and Dsui_0007 are separated by 85 nucleotides
Dsui_0003: Dsui_0003 - putative Zn-dependent protease, at 2,299 to 3,786
_0003
Dsui_0004: Dsui_0004 - molybdenum cofactor biosynthesis protein MoaC, at 3,829 to 4,323
_0004
Dsui_0005: Dsui_0005 - putative secreted protein, at 4,356 to 4,826
_0005
Dsui_0006: Dsui_0006 - sulfur oxidation protein SoxZ, at 4,836 to 5,153
_0006
Dsui_0007: Dsui_0007 - cold shock protein, at 5,239 to 5,442
_0007
Position (kb) 3
4
5 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 2.977 kb on - strand, within Dsui_0003 at 3.026 kb on - strand, within Dsui_0003 at 3.131 kb on - strand at 3.222 kb on - strand, within Dsui_0003 at 3.413 kb on - strand, within Dsui_0003 at 3.503 kb on - strand, within Dsui_0003 at 3.503 kb on - strand at 3.503 kb on - strand, within Dsui_0003 at 3.567 kb on - strand, within Dsui_0003 at 3.791 kb on + strand at 3.833 kb on - strand at 3.833 kb on - strand at 3.861 kb on - strand at 4.008 kb on + strand, within Dsui_0004 at 4.035 kb on + strand, within Dsui_0004 at 4.079 kb on + strand, within Dsui_0004 at 4.093 kb on - strand at 4.099 kb on + strand, within Dsui_0004 at 4.205 kb on + strand, within Dsui_0004 at 4.230 kb on - strand, within Dsui_0004 at 4.241 kb on - strand at 4.260 kb on + strand, within Dsui_0004 at 4.426 kb on - strand, within Dsui_0005 at 4.426 kb on - strand at 4.504 kb on - strand, within Dsui_0005 at 4.522 kb on - strand, within Dsui_0005 at 4.630 kb on + strand, within Dsui_0005 at 4.631 kb on - strand, within Dsui_0005 at 4.695 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.705 kb on + strand, within Dsui_0005 at 4.713 kb on - strand, within Dsui_0005 at 4.725 kb on - strand, within Dsui_0005 at 4.742 kb on + strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.750 kb on - strand, within Dsui_0005 at 4.762 kb on - strand at 4.861 kb on + strand at 4.887 kb on + strand, within Dsui_0006 at 4.893 kb on - strand, within Dsui_0006 at 4.910 kb on + strand, within Dsui_0006 at 5.002 kb on + strand, within Dsui_0006 at 5.002 kb on - strand, within Dsui_0006 at 5.007 kb on + strand, within Dsui_0006 at 5.007 kb on + strand, within Dsui_0006 at 5.026 kb on - strand, within Dsui_0006 at 5.077 kb on - strand at 5.123 kb on - strand at 5.123 kb on - strand at 5.164 kb on + strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.172 kb on - strand at 5.173 kb on - strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.237 kb on + strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.244 kb on - strand at 5.297 kb on - strand, within Dsui_0007
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 2,977 - Dsui_0003 0.46 -3.3 3,026 - Dsui_0003 0.49 -2.5 3,131 - -1.1 3,222 - Dsui_0003 0.62 -0.7 3,413 - Dsui_0003 0.75 -2.0 3,503 - Dsui_0003 0.81 +0.5 3,503 - -2.3 3,503 - Dsui_0003 0.81 -2.6 3,567 - Dsui_0003 0.85 -3.9 3,791 + +0.5 3,833 - -4.1 3,833 - -2.7 3,861 - -5.2 4,008 + Dsui_0004 0.36 -3.9 4,035 + Dsui_0004 0.42 -4.2 4,079 + Dsui_0004 0.51 -7.3 4,093 - -2.1 4,099 + Dsui_0004 0.55 -2.6 4,205 + Dsui_0004 0.76 -5.8 4,230 - Dsui_0004 0.81 -5.2 4,241 - -2.8 4,260 + Dsui_0004 0.87 -6.9 4,426 - Dsui_0005 0.15 +1.1 4,426 - -0.6 4,504 - Dsui_0005 0.31 +1.9 4,522 - Dsui_0005 0.35 -0.0 4,630 + Dsui_0005 0.58 +1.6 4,631 - Dsui_0005 0.58 +2.0 4,695 + Dsui_0005 0.72 +0.9 4,705 + Dsui_0005 0.74 +0.6 4,705 + Dsui_0005 0.74 +1.0 4,705 + Dsui_0005 0.74 +1.6 4,705 + Dsui_0005 0.74 +1.4 4,713 - Dsui_0005 0.76 -1.9 4,725 - Dsui_0005 0.78 -0.5 4,742 + Dsui_0005 0.82 +1.7 4,750 - Dsui_0005 0.84 -0.7 4,750 - Dsui_0005 0.84 -3.1 4,750 - Dsui_0005 0.84 -0.6 4,750 - Dsui_0005 0.84 +0.4 4,762 - +0.9 4,861 + +0.6 4,887 + Dsui_0006 0.16 -0.5 4,893 - Dsui_0006 0.18 +0.6 4,910 + Dsui_0006 0.23 +1.0 5,002 + Dsui_0006 0.52 +1.1 5,002 - Dsui_0006 0.52 +0.4 5,007 + Dsui_0006 0.54 +1.6 5,007 + Dsui_0006 0.54 +1.0 5,026 - Dsui_0006 0.60 +0.2 5,077 - -1.0 5,123 - +0.3 5,123 - +1.7 5,164 + +0.7 5,172 - +1.7 5,172 - +0.9 5,172 - +1.1 5,173 - +0.2 5,237 + -1.0 5,237 + -3.2 5,237 + +1.0 5,244 - -1.1 5,244 - -0.8 5,244 - -1.5 5,297 - Dsui_0007 0.28 -1.1
Or see this region's nucleotide sequence