Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_27930 and AO353_27935 are separated by 148 nucleotides AO353_27935 and AO353_27940 are separated by 35 nucleotides AO353_27940 and AO353_27945 are separated by 23 nucleotides
AO353_27930: AO353_27930 - LysR family transcriptional regulator, at 6,093,939 to 6,094,823
_27930
AO353_27935: AO353_27935 - succinyl-CoA--3-ketoacid-CoA transferase, at 6,094,972 to 6,095,670
_27935
AO353_27940: AO353_27940 - succinyl-CoA--3-ketoacid-CoA transferase, at 6,095,706 to 6,096,365
_27940
AO353_27945: AO353_27945 - acetyl-CoA acetyltransferase, at 6,096,389 to 6,097,570
_27945
Position (kb)
6094
6095
6096 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6094.055 kb on + strand, within AO353_27930 at 6094.055 kb on + strand, within AO353_27930 at 6094.056 kb on - strand, within AO353_27930 at 6094.056 kb on - strand, within AO353_27930 at 6094.056 kb on - strand, within AO353_27930 at 6094.056 kb on - strand, within AO353_27930 at 6094.251 kb on - strand, within AO353_27930 at 6094.251 kb on - strand, within AO353_27930 at 6094.251 kb on - strand, within AO353_27930 at 6094.342 kb on + strand, within AO353_27930 at 6094.342 kb on + strand, within AO353_27930 at 6094.342 kb on + strand, within AO353_27930 at 6094.342 kb on + strand, within AO353_27930 at 6094.343 kb on - strand, within AO353_27930 at 6094.343 kb on - strand, within AO353_27930 at 6094.629 kb on + strand, within AO353_27930 at 6094.896 kb on - strand at 6094.959 kb on - strand at 6094.972 kb on - strand at 6094.972 kb on - strand at 6095.002 kb on + strand at 6095.002 kb on + strand at 6095.002 kb on + strand at 6095.003 kb on - strand at 6095.003 kb on - strand at 6095.003 kb on - strand at 6095.034 kb on + strand at 6095.034 kb on + strand at 6095.034 kb on + strand at 6095.035 kb on - strand at 6095.035 kb on - strand at 6095.035 kb on - strand at 6095.035 kb on - strand at 6095.035 kb on - strand at 6095.035 kb on - strand at 6095.070 kb on + strand, within AO353_27935 at 6095.169 kb on - strand, within AO353_27935 at 6095.209 kb on + strand, within AO353_27935 at 6095.209 kb on + strand, within AO353_27935 at 6095.209 kb on + strand, within AO353_27935 at 6095.210 kb on - strand, within AO353_27935 at 6095.210 kb on - strand, within AO353_27935 at 6095.210 kb on - strand, within AO353_27935 at 6095.210 kb on - strand at 6095.210 kb on - strand, within AO353_27935 at 6095.210 kb on - strand, within AO353_27935 at 6095.480 kb on - strand at 6095.605 kb on + strand at 6095.605 kb on + strand at 6095.605 kb on + strand at 6095.605 kb on + strand at 6095.605 kb on + strand at 6095.605 kb on + strand at 6095.605 kb on + strand at 6095.605 kb on + strand at 6095.605 kb on + strand at 6095.606 kb on - strand at 6095.606 kb on - strand at 6095.606 kb on - strand at 6095.606 kb on - strand at 6095.606 kb on - strand at 6095.606 kb on - strand at 6095.606 kb on - strand at 6095.644 kb on - strand at 6095.714 kb on + strand at 6095.764 kb on - strand at 6095.766 kb on + strand at 6095.767 kb on - strand at 6095.767 kb on - strand at 6095.767 kb on - strand at 6095.767 kb on - strand at 6095.767 kb on - strand at 6095.808 kb on + strand, within AO353_27940 at 6095.808 kb on + strand, within AO353_27940 at 6095.808 kb on + strand, within AO353_27940 at 6095.808 kb on + strand, within AO353_27940 at 6095.808 kb on + strand, within AO353_27940 at 6095.808 kb on + strand, within AO353_27940 at 6095.808 kb on + strand, within AO353_27940 at 6095.808 kb on + strand, within AO353_27940 at 6095.808 kb on + strand, within AO353_27940 at 6095.809 kb on - strand, within AO353_27940 at 6095.809 kb on - strand, within AO353_27940 at 6095.809 kb on - strand, within AO353_27940 at 6095.885 kb on + strand, within AO353_27940 at 6096.031 kb on + strand, within AO353_27940 at 6096.032 kb on - strand, within AO353_27940 at 6096.032 kb on - strand, within AO353_27940 at 6096.032 kb on - strand, within AO353_27940 at 6096.174 kb on + strand, within AO353_27940 at 6096.175 kb on - strand, within AO353_27940 at 6096.234 kb on + strand, within AO353_27940 at 6096.320 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 6,094,055 + AO353_27930 0.13 +0.0 6,094,055 + AO353_27930 0.13 +1.7 6,094,056 - AO353_27930 0.13 -0.6 6,094,056 - AO353_27930 0.13 -2.1 6,094,056 - AO353_27930 0.13 +0.0 6,094,056 - AO353_27930 0.13 +0.7 6,094,251 - AO353_27930 0.35 -1.6 6,094,251 - AO353_27930 0.35 +0.7 6,094,251 - AO353_27930 0.35 -0.3 6,094,342 + AO353_27930 0.46 -0.5 6,094,342 + AO353_27930 0.46 -0.8 6,094,342 + AO353_27930 0.46 -0.1 6,094,342 + AO353_27930 0.46 -1.1 6,094,343 - AO353_27930 0.46 -0.8 6,094,343 - AO353_27930 0.46 -1.8 6,094,629 + AO353_27930 0.78 +1.1 6,094,896 - +0.0 6,094,959 - +0.7 6,094,972 - -0.8 6,094,972 - +0.5 6,095,002 + +1.1 6,095,002 + +0.6 6,095,002 + -0.5 6,095,003 - +0.1 6,095,003 - -1.3 6,095,003 - +1.7 6,095,034 + +0.7 6,095,034 + +0.1 6,095,034 + -1.4 6,095,035 - +0.2 6,095,035 - -1.3 6,095,035 - +0.7 6,095,035 - +0.3 6,095,035 - -2.3 6,095,035 - +2.3 6,095,070 + AO353_27935 0.14 -0.7 6,095,169 - AO353_27935 0.28 +0.2 6,095,209 + AO353_27935 0.34 +1.0 6,095,209 + AO353_27935 0.34 -0.3 6,095,209 + AO353_27935 0.34 -1.3 6,095,210 - AO353_27935 0.34 +1.0 6,095,210 - AO353_27935 0.34 +1.2 6,095,210 - AO353_27935 0.34 +1.7 6,095,210 - -0.8 6,095,210 - AO353_27935 0.34 -0.1 6,095,210 - AO353_27935 0.34 -1.1 6,095,480 - +0.7 6,095,605 + -1.6 6,095,605 + -0.6 6,095,605 + +0.7 6,095,605 + -0.8 6,095,605 + +3.7 6,095,605 + -1.1 6,095,605 + -1.3 6,095,605 + +0.3 6,095,605 + +1.0 6,095,606 - -0.7 6,095,606 - +1.6 6,095,606 - -1.4 6,095,606 - -1.6 6,095,606 - +0.2 6,095,606 - -0.3 6,095,606 - -0.3 6,095,644 - +0.2 6,095,714 + +0.3 6,095,764 - -1.1 6,095,766 + -2.6 6,095,767 - +1.5 6,095,767 - +0.7 6,095,767 - +0.2 6,095,767 - +0.7 6,095,767 - -0.8 6,095,808 + AO353_27940 0.15 -1.8 6,095,808 + AO353_27940 0.15 +2.3 6,095,808 + AO353_27940 0.15 +0.4 6,095,808 + AO353_27940 0.15 -0.3 6,095,808 + AO353_27940 0.15 -0.4 6,095,808 + AO353_27940 0.15 +0.3 6,095,808 + AO353_27940 0.15 -0.8 6,095,808 + AO353_27940 0.15 +0.3 6,095,808 + AO353_27940 0.15 -0.3 6,095,809 - AO353_27940 0.16 +1.7 6,095,809 - AO353_27940 0.16 +0.7 6,095,809 - AO353_27940 0.16 +0.2 6,095,885 + AO353_27940 0.27 +0.9 6,096,031 + AO353_27940 0.49 +0.3 6,096,032 - AO353_27940 0.49 -0.3 6,096,032 - AO353_27940 0.49 +0.1 6,096,032 - AO353_27940 0.49 +0.7 6,096,174 + AO353_27940 0.71 +0.7 6,096,175 - AO353_27940 0.71 +0.1 6,096,234 + AO353_27940 0.80 -1.1 6,096,320 + +0.7
Or see this region's nucleotide sequence