Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_21630 and AO353_21635 are separated by 81 nucleotides AO353_21635 and AO353_21640 overlap by 4 nucleotides AO353_21640 and AO353_21645 are separated by 11 nucleotides AO353_21645 and AO353_21650 are separated by 331 nucleotides
AO353_21630: AO353_21630 - precorrin-4 C11-methyltransferase, at 4,743,371 to 4,744,117
_21630
AO353_21635: AO353_21635 - cobalamin biosynthesis protein CobE, at 4,744,199 to 4,744,612
_21635
AO353_21640: AO353_21640 - cobalt transporter, at 4,744,609 to 4,745,310
_21640
AO353_21645: AO353_21645 - cobalt transporter, at 4,745,322 to 4,745,513
_21645
AO353_21650: AO353_21650 - hypothetical protein, at 4,745,845 to 4,746,066
_21650
Position (kb)
4744
4745
4746 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4743.996 kb on - strand, within AO353_21630 at 4744.008 kb on - strand, within AO353_21630 at 4744.008 kb on - strand, within AO353_21630 at 4744.023 kb on + strand, within AO353_21630 at 4744.023 kb on + strand, within AO353_21630 at 4744.107 kb on + strand at 4744.107 kb on + strand at 4744.107 kb on + strand at 4744.108 kb on - strand at 4744.108 kb on - strand at 4744.236 kb on + strand at 4744.237 kb on - strand at 4744.320 kb on + strand, within AO353_21635 at 4744.374 kb on + strand, within AO353_21635 at 4744.493 kb on + strand, within AO353_21635 at 4744.493 kb on + strand at 4744.493 kb on + strand, within AO353_21635 at 4744.494 kb on - strand, within AO353_21635 at 4744.494 kb on - strand, within AO353_21635 at 4745.073 kb on + strand, within AO353_21640 at 4745.198 kb on + strand, within AO353_21640 at 4745.298 kb on + strand at 4745.299 kb on - strand at 4745.299 kb on - strand at 4745.404 kb on + strand, within AO353_21645 at 4745.404 kb on + strand, within AO353_21645 at 4745.404 kb on + strand, within AO353_21645 at 4745.404 kb on + strand, within AO353_21645 at 4745.404 kb on + strand, within AO353_21645 at 4745.404 kb on + strand, within AO353_21645 at 4745.404 kb on + strand, within AO353_21645 at 4745.405 kb on - strand, within AO353_21645 at 4745.405 kb on - strand, within AO353_21645 at 4745.556 kb on + strand at 4745.556 kb on + strand at 4745.556 kb on + strand at 4745.556 kb on + strand at 4745.557 kb on - strand at 4745.557 kb on - strand at 4745.557 kb on - strand at 4745.557 kb on - strand at 4745.557 kb on - strand at 4745.557 kb on - strand at 4745.616 kb on + strand at 4745.617 kb on - strand at 4745.774 kb on + strand at 4745.774 kb on + strand at 4745.774 kb on + strand at 4745.825 kb on - strand at 4745.836 kb on + strand at 4745.836 kb on + strand at 4745.837 kb on - strand at 4745.837 kb on - strand at 4745.837 kb on - strand at 4745.963 kb on + strand, within AO353_21650 at 4745.964 kb on - strand, within AO353_21650 at 4746.106 kb on + strand at 4746.106 kb on + strand at 4746.106 kb on + strand at 4746.106 kb on + strand at 4746.106 kb on + strand at 4746.107 kb on - strand at 4746.107 kb on - strand at 4746.107 kb on - strand at 4746.107 kb on - strand at 4746.107 kb on - strand at 4746.107 kb on - strand at 4746.275 kb on + strand at 4746.275 kb on + strand at 4746.276 kb on - strand at 4746.276 kb on - strand at 4746.276 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,743,996 - AO353_21630 0.84 +0.5 4,744,008 - AO353_21630 0.85 +0.8 4,744,008 - AO353_21630 0.85 +1.0 4,744,023 + AO353_21630 0.87 -2.4 4,744,023 + AO353_21630 0.87 +1.0 4,744,107 + +1.4 4,744,107 + -0.2 4,744,107 + +0.5 4,744,108 - +0.5 4,744,108 - +0.1 4,744,236 + -0.8 4,744,237 - -0.2 4,744,320 + AO353_21635 0.29 -0.4 4,744,374 + AO353_21635 0.42 -0.0 4,744,493 + AO353_21635 0.71 -0.2 4,744,493 + -1.5 4,744,493 + AO353_21635 0.71 -0.6 4,744,494 - AO353_21635 0.71 +0.1 4,744,494 - AO353_21635 0.71 -0.4 4,745,073 + AO353_21640 0.66 -0.2 4,745,198 + AO353_21640 0.84 -1.4 4,745,298 + +1.2 4,745,299 - +0.3 4,745,299 - +0.6 4,745,404 + AO353_21645 0.43 -0.7 4,745,404 + AO353_21645 0.43 +0.8 4,745,404 + AO353_21645 0.43 -1.5 4,745,404 + AO353_21645 0.43 +0.8 4,745,404 + AO353_21645 0.43 -1.2 4,745,404 + AO353_21645 0.43 +0.1 4,745,404 + AO353_21645 0.43 +0.4 4,745,405 - AO353_21645 0.43 +1.4 4,745,405 - AO353_21645 0.43 +2.8 4,745,556 + -0.2 4,745,556 + -0.4 4,745,556 + -0.5 4,745,556 + -0.7 4,745,557 - -1.2 4,745,557 - -0.5 4,745,557 - -1.4 4,745,557 - -0.1 4,745,557 - +0.8 4,745,557 - -0.7 4,745,616 + -0.5 4,745,617 - +0.2 4,745,774 + -0.2 4,745,774 + -0.0 4,745,774 + -0.0 4,745,825 - -1.8 4,745,836 + +0.4 4,745,836 + -1.1 4,745,837 - +0.1 4,745,837 - -0.8 4,745,837 - -1.9 4,745,963 + AO353_21650 0.53 +0.8 4,745,964 - AO353_21650 0.54 +1.8 4,746,106 + -0.1 4,746,106 + +0.8 4,746,106 + +0.1 4,746,106 + -1.3 4,746,106 + +1.3 4,746,107 - +0.8 4,746,107 - -0.7 4,746,107 - -0.2 4,746,107 - +1.3 4,746,107 - +0.8 4,746,107 - -0.4 4,746,275 + +1.0 4,746,275 + -1.2 4,746,276 - -1.4 4,746,276 - -1.0 4,746,276 - -1.2
Or see this region's nucleotide sequence