Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gidA and Synpcc7942_2424 are separated by 48 nucleotides Synpcc7942_2424 and ycf39 are separated by 71 nucleotides ycf39 and petM are separated by 118 nucleotides petM and Synpcc7942_2427 are separated by 78 nucleotides
Synpcc7942_2423: gidA - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 2,494,868 to 2,496,775
gidA
Synpcc7942_2424: Synpcc7942_2424 - hypothetical protein, at 2,496,824 to 2,497,627
_2424
Synpcc7942_2425: ycf39 - chaperon-like protein for quinone binding in photosystem II, at 2,497,699 to 2,498,661
ycf39
Synpcc7942_2426: petM - cytochrome b6f complex subunit PetM, at 2,498,780 to 2,498,893
petM
Synpcc7942_2427: Synpcc7942_2427 - 3-mercaptopyruvate sulfurtransferase, at 2,498,972 to 2,499,814
_2427
Position (kb)
2497
2498
2499 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2496.796 kb on - strand at 2496.862 kb on - strand at 2496.864 kb on + strand at 2496.864 kb on + strand at 2496.872 kb on - strand at 2496.885 kb on - strand at 2496.887 kb on + strand at 2496.903 kb on + strand at 2496.921 kb on - strand, within Synpcc7942_2424 at 2496.921 kb on - strand, within Synpcc7942_2424 at 2496.935 kb on + strand, within Synpcc7942_2424 at 2496.946 kb on + strand, within Synpcc7942_2424 at 2496.954 kb on - strand, within Synpcc7942_2424 at 2496.971 kb on + strand, within Synpcc7942_2424 at 2496.979 kb on - strand, within Synpcc7942_2424 at 2497.005 kb on + strand, within Synpcc7942_2424 at 2497.040 kb on - strand at 2497.041 kb on - strand, within Synpcc7942_2424 at 2497.054 kb on + strand, within Synpcc7942_2424 at 2497.076 kb on + strand, within Synpcc7942_2424 at 2497.076 kb on + strand, within Synpcc7942_2424 at 2497.083 kb on + strand at 2497.083 kb on - strand, within Synpcc7942_2424 at 2497.084 kb on - strand, within Synpcc7942_2424 at 2497.084 kb on - strand, within Synpcc7942_2424 at 2497.084 kb on - strand, within Synpcc7942_2424 at 2497.085 kb on + strand, within Synpcc7942_2424 at 2497.091 kb on + strand, within Synpcc7942_2424 at 2497.099 kb on - strand, within Synpcc7942_2424 at 2497.126 kb on + strand, within Synpcc7942_2424 at 2497.136 kb on + strand, within Synpcc7942_2424 at 2497.230 kb on - strand, within Synpcc7942_2424 at 2497.247 kb on + strand, within Synpcc7942_2424 at 2497.255 kb on - strand, within Synpcc7942_2424 at 2497.290 kb on + strand, within Synpcc7942_2424 at 2497.292 kb on + strand, within Synpcc7942_2424 at 2497.292 kb on + strand, within Synpcc7942_2424 at 2497.297 kb on + strand, within Synpcc7942_2424 at 2497.298 kb on + strand, within Synpcc7942_2424 at 2497.305 kb on - strand, within Synpcc7942_2424 at 2497.305 kb on - strand, within Synpcc7942_2424 at 2497.305 kb on - strand, within Synpcc7942_2424 at 2497.317 kb on + strand, within Synpcc7942_2424 at 2497.339 kb on - strand, within Synpcc7942_2424 at 2497.342 kb on - strand, within Synpcc7942_2424 at 2497.344 kb on - strand, within Synpcc7942_2424 at 2497.344 kb on - strand, within Synpcc7942_2424 at 2497.357 kb on - strand, within Synpcc7942_2424 at 2497.424 kb on + strand, within Synpcc7942_2424 at 2497.426 kb on + strand, within Synpcc7942_2424 at 2497.430 kb on + strand, within Synpcc7942_2424 at 2497.461 kb on + strand, within Synpcc7942_2424 at 2497.471 kb on + strand, within Synpcc7942_2424 at 2497.471 kb on + strand, within Synpcc7942_2424 at 2497.479 kb on - strand, within Synpcc7942_2424 at 2497.479 kb on - strand, within Synpcc7942_2424 at 2497.479 kb on - strand, within Synpcc7942_2424 at 2497.479 kb on - strand, within Synpcc7942_2424 at 2497.487 kb on - strand, within Synpcc7942_2424 at 2497.510 kb on - strand, within Synpcc7942_2424 at 2497.530 kb on - strand, within Synpcc7942_2424 at 2497.532 kb on - strand, within Synpcc7942_2424 at 2497.565 kb on - strand at 2497.639 kb on - strand at 2497.662 kb on + strand at 2497.662 kb on + strand at 2497.662 kb on + strand at 2497.662 kb on + strand at 2497.668 kb on - strand at 2497.668 kb on - strand at 2497.670 kb on - strand at 2497.670 kb on - strand at 2497.670 kb on - strand at 2497.670 kb on - strand at 2497.670 kb on - strand at 2497.670 kb on - strand at 2497.683 kb on - strand at 2497.697 kb on + strand at 2497.697 kb on + strand at 2497.705 kb on - strand at 2497.749 kb on + strand at 2497.759 kb on - strand at 2497.795 kb on + strand at 2497.795 kb on - strand at 2497.847 kb on + strand, within ycf39 at 2497.902 kb on - strand, within ycf39 at 2497.929 kb on - strand, within ycf39 at 2497.953 kb on + strand, within ycf39 at 2497.990 kb on - strand, within ycf39 at 2498.097 kb on - strand, within ycf39 at 2498.118 kb on + strand, within ycf39 at 2498.121 kb on - strand, within ycf39 at 2498.214 kb on - strand, within ycf39 at 2498.225 kb on - strand, within ycf39 at 2498.282 kb on - strand, within ycf39 at 2498.362 kb on + strand, within ycf39 at 2498.375 kb on + strand, within ycf39 at 2498.383 kb on - strand, within ycf39 at 2498.385 kb on + strand, within ycf39 at 2498.430 kb on - strand, within ycf39 at 2498.468 kb on + strand, within ycf39 at 2498.556 kb on + strand, within ycf39 at 2498.556 kb on + strand, within ycf39 at 2498.564 kb on - strand, within ycf39 at 2498.564 kb on - strand, within ycf39 at 2498.564 kb on - strand, within ycf39 at 2498.576 kb on - strand at 2498.577 kb on + strand at 2498.659 kb on + strand at 2498.704 kb on + strand at 2498.716 kb on + strand at 2498.726 kb on + strand at 2498.741 kb on + strand at 2498.755 kb on + strand at 2498.765 kb on - strand at 2498.770 kb on - strand at 2498.862 kb on - strand, within petM at 2498.902 kb on - strand at 2498.915 kb on - strand at 2498.925 kb on - strand at 2498.968 kb on + strand at 2498.981 kb on + strand at 2498.986 kb on + strand at 2498.989 kb on - strand at 2498.991 kb on + strand at 2499.035 kb on - strand at 2499.054 kb on + strand at 2499.057 kb on - strand, within Synpcc7942_2427 at 2499.064 kb on + strand, within Synpcc7942_2427 at 2499.068 kb on + strand, within Synpcc7942_2427 at 2499.072 kb on - strand, within Synpcc7942_2427 at 2499.075 kb on + strand, within Synpcc7942_2427 at 2499.076 kb on - strand at 2499.076 kb on - strand, within Synpcc7942_2427 at 2499.085 kb on + strand, within Synpcc7942_2427 at 2499.098 kb on + strand, within Synpcc7942_2427 at 2499.103 kb on + strand, within Synpcc7942_2427 at 2499.103 kb on - strand, within Synpcc7942_2427 at 2499.104 kb on - strand, within Synpcc7942_2427 at 2499.106 kb on - strand, within Synpcc7942_2427 at 2499.151 kb on - strand, within Synpcc7942_2427 at 2499.173 kb on - strand, within Synpcc7942_2427 at 2499.174 kb on - strand, within Synpcc7942_2427 at 2499.176 kb on + strand, within Synpcc7942_2427 at 2499.190 kb on + strand, within Synpcc7942_2427 at 2499.193 kb on - strand, within Synpcc7942_2427 at 2499.198 kb on - strand, within Synpcc7942_2427 at 2499.198 kb on - strand, within Synpcc7942_2427 at 2499.211 kb on - strand, within Synpcc7942_2427 at 2499.212 kb on - strand, within Synpcc7942_2427 at 2499.237 kb on - strand, within Synpcc7942_2427 at 2499.240 kb on + strand, within Synpcc7942_2427 at 2499.291 kb on + strand, within Synpcc7942_2427 at 2499.301 kb on + strand, within Synpcc7942_2427 at 2499.309 kb on - strand, within Synpcc7942_2427 at 2499.337 kb on - strand, within Synpcc7942_2427 at 2499.367 kb on - strand, within Synpcc7942_2427 at 2499.417 kb on - strand, within Synpcc7942_2427 at 2499.429 kb on - strand, within Synpcc7942_2427 at 2499.461 kb on - strand, within Synpcc7942_2427 at 2499.461 kb on - strand, within Synpcc7942_2427 at 2499.461 kb on - strand, within Synpcc7942_2427 at 2499.464 kb on + strand, within Synpcc7942_2427 at 2499.469 kb on + strand, within Synpcc7942_2427 at 2499.490 kb on - strand, within Synpcc7942_2427 at 2499.511 kb on + strand, within Synpcc7942_2427 at 2499.587 kb on + strand at 2499.595 kb on - strand, within Synpcc7942_2427 at 2499.601 kb on + strand, within Synpcc7942_2427 at 2499.657 kb on - strand, within Synpcc7942_2427 at 2499.657 kb on - strand, within Synpcc7942_2427
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 2,496,796 - +1.8 2,496,862 - -0.8 2,496,864 + -0.6 2,496,864 + +2.5 2,496,872 - +0.9 2,496,885 - +0.6 2,496,887 + +0.8 2,496,903 + -2.6 2,496,921 - Synpcc7942_2424 0.12 +0.2 2,496,921 - Synpcc7942_2424 0.12 -0.6 2,496,935 + Synpcc7942_2424 0.14 -1.9 2,496,946 + Synpcc7942_2424 0.15 +0.5 2,496,954 - Synpcc7942_2424 0.16 +0.5 2,496,971 + Synpcc7942_2424 0.18 -0.2 2,496,979 - Synpcc7942_2424 0.19 +2.0 2,497,005 + Synpcc7942_2424 0.23 -0.8 2,497,040 - -0.1 2,497,041 - Synpcc7942_2424 0.27 -0.4 2,497,054 + Synpcc7942_2424 0.29 -1.6 2,497,076 + Synpcc7942_2424 0.31 +0.2 2,497,076 + Synpcc7942_2424 0.31 +0.3 2,497,083 + -0.1 2,497,083 - Synpcc7942_2424 0.32 -0.2 2,497,084 - Synpcc7942_2424 0.32 +0.8 2,497,084 - Synpcc7942_2424 0.32 -0.6 2,497,084 - Synpcc7942_2424 0.32 -1.4 2,497,085 + Synpcc7942_2424 0.32 -0.1 2,497,091 + Synpcc7942_2424 0.33 +0.2 2,497,099 - Synpcc7942_2424 0.34 +1.4 2,497,126 + Synpcc7942_2424 0.38 +0.3 2,497,136 + Synpcc7942_2424 0.39 -0.5 2,497,230 - Synpcc7942_2424 0.50 +0.4 2,497,247 + Synpcc7942_2424 0.53 -0.5 2,497,255 - Synpcc7942_2424 0.54 +0.7 2,497,290 + Synpcc7942_2424 0.58 +1.9 2,497,292 + Synpcc7942_2424 0.58 -1.3 2,497,292 + Synpcc7942_2424 0.58 +0.1 2,497,297 + Synpcc7942_2424 0.59 -1.5 2,497,298 + Synpcc7942_2424 0.59 -0.9 2,497,305 - Synpcc7942_2424 0.60 +0.5 2,497,305 - Synpcc7942_2424 0.60 -0.5 2,497,305 - Synpcc7942_2424 0.60 +0.8 2,497,317 + Synpcc7942_2424 0.61 -1.0 2,497,339 - Synpcc7942_2424 0.64 +0.2 2,497,342 - Synpcc7942_2424 0.64 +0.5 2,497,344 - Synpcc7942_2424 0.65 +0.5 2,497,344 - Synpcc7942_2424 0.65 -0.3 2,497,357 - Synpcc7942_2424 0.66 +0.2 2,497,424 + Synpcc7942_2424 0.75 +0.8 2,497,426 + Synpcc7942_2424 0.75 +0.3 2,497,430 + Synpcc7942_2424 0.75 +0.7 2,497,461 + Synpcc7942_2424 0.79 -1.6 2,497,471 + Synpcc7942_2424 0.80 -0.1 2,497,471 + Synpcc7942_2424 0.80 +0.6 2,497,479 - Synpcc7942_2424 0.81 +0.4 2,497,479 - Synpcc7942_2424 0.81 -2.4 2,497,479 - Synpcc7942_2424 0.81 +0.9 2,497,479 - Synpcc7942_2424 0.81 +0.3 2,497,487 - Synpcc7942_2424 0.82 +0.9 2,497,510 - Synpcc7942_2424 0.85 +0.7 2,497,530 - Synpcc7942_2424 0.88 +0.3 2,497,532 - Synpcc7942_2424 0.88 -1.1 2,497,565 - -0.1 2,497,639 - -0.5 2,497,662 + -1.5 2,497,662 + -0.6 2,497,662 + -2.2 2,497,662 + -0.1 2,497,668 - +1.5 2,497,668 - -0.1 2,497,670 - -0.1 2,497,670 - +0.6 2,497,670 - +1.4 2,497,670 - +0.1 2,497,670 - -0.1 2,497,670 - -0.2 2,497,683 - +1.3 2,497,697 + -0.1 2,497,697 + +1.0 2,497,705 - -0.8 2,497,749 + -3.2 2,497,759 - -1.3 2,497,795 + -2.4 2,497,795 - -1.1 2,497,847 + ycf39 Synpcc7942_2425 0.15 -2.6 2,497,902 - ycf39 Synpcc7942_2425 0.21 -2.6 2,497,929 - ycf39 Synpcc7942_2425 0.24 -2.6 2,497,953 + ycf39 Synpcc7942_2425 0.26 -3.1 2,497,990 - ycf39 Synpcc7942_2425 0.30 -2.6 2,498,097 - ycf39 Synpcc7942_2425 0.41 -1.3 2,498,118 + ycf39 Synpcc7942_2425 0.44 -0.5 2,498,121 - ycf39 Synpcc7942_2425 0.44 -1.1 2,498,214 - ycf39 Synpcc7942_2425 0.53 -2.4 2,498,225 - ycf39 Synpcc7942_2425 0.55 -1.1 2,498,282 - ycf39 Synpcc7942_2425 0.61 -2.6 2,498,362 + ycf39 Synpcc7942_2425 0.69 +0.5 2,498,375 + ycf39 Synpcc7942_2425 0.70 -3.3 2,498,383 - ycf39 Synpcc7942_2425 0.71 +1.5 2,498,385 + ycf39 Synpcc7942_2425 0.71 -0.1 2,498,430 - ycf39 Synpcc7942_2425 0.76 -0.4 2,498,468 + ycf39 Synpcc7942_2425 0.80 -2.1 2,498,556 + ycf39 Synpcc7942_2425 0.89 -0.7 2,498,556 + ycf39 Synpcc7942_2425 0.89 -2.1 2,498,564 - ycf39 Synpcc7942_2425 0.90 -2.4 2,498,564 - ycf39 Synpcc7942_2425 0.90 -0.1 2,498,564 - ycf39 Synpcc7942_2425 0.90 -1.6 2,498,576 - -2.1 2,498,577 + -0.1 2,498,659 + +0.2 2,498,704 + +1.2 2,498,716 + -0.8 2,498,726 + -0.5 2,498,741 + +0.2 2,498,755 + -0.6 2,498,765 - +0.1 2,498,770 - +1.1 2,498,862 - petM Synpcc7942_2426 0.72 -2.6 2,498,902 - -0.1 2,498,915 - -1.6 2,498,925 - -2.9 2,498,968 + +0.3 2,498,981 + -0.6 2,498,986 + +0.7 2,498,989 - +0.3 2,498,991 + +0.4 2,499,035 - -1.1 2,499,054 + +0.8 2,499,057 - Synpcc7942_2427 0.10 +0.9 2,499,064 + Synpcc7942_2427 0.11 -0.9 2,499,068 + Synpcc7942_2427 0.11 +0.7 2,499,072 - Synpcc7942_2427 0.12 +0.5 2,499,075 + Synpcc7942_2427 0.12 -0.1 2,499,076 - +1.5 2,499,076 - Synpcc7942_2427 0.12 -1.6 2,499,085 + Synpcc7942_2427 0.13 +0.5 2,499,098 + Synpcc7942_2427 0.15 +0.9 2,499,103 + Synpcc7942_2427 0.16 +0.2 2,499,103 - Synpcc7942_2427 0.16 +0.9 2,499,104 - Synpcc7942_2427 0.16 +1.4 2,499,106 - Synpcc7942_2427 0.16 +0.5 2,499,151 - Synpcc7942_2427 0.21 +0.5 2,499,173 - Synpcc7942_2427 0.24 -0.2 2,499,174 - Synpcc7942_2427 0.24 +0.4 2,499,176 + Synpcc7942_2427 0.24 -0.2 2,499,190 + Synpcc7942_2427 0.26 -1.1 2,499,193 - Synpcc7942_2427 0.26 -0.1 2,499,198 - Synpcc7942_2427 0.27 +1.2 2,499,198 - Synpcc7942_2427 0.27 -0.2 2,499,211 - Synpcc7942_2427 0.28 -0.6 2,499,212 - Synpcc7942_2427 0.28 +0.6 2,499,237 - Synpcc7942_2427 0.31 -0.5 2,499,240 + Synpcc7942_2427 0.32 +1.7 2,499,291 + Synpcc7942_2427 0.38 +1.3 2,499,301 + Synpcc7942_2427 0.39 +0.3 2,499,309 - Synpcc7942_2427 0.40 +0.5 2,499,337 - Synpcc7942_2427 0.43 -1.6 2,499,367 - Synpcc7942_2427 0.47 -0.7 2,499,417 - Synpcc7942_2427 0.53 +1.3 2,499,429 - Synpcc7942_2427 0.54 +0.5 2,499,461 - Synpcc7942_2427 0.58 -0.1 2,499,461 - Synpcc7942_2427 0.58 -2.4 2,499,461 - Synpcc7942_2427 0.58 +0.0 2,499,464 + Synpcc7942_2427 0.58 -1.1 2,499,469 + Synpcc7942_2427 0.59 -1.1 2,499,490 - Synpcc7942_2427 0.61 +0.2 2,499,511 + Synpcc7942_2427 0.64 -0.3 2,499,587 + -0.1 2,499,595 - Synpcc7942_2427 0.74 +1.7 2,499,601 + Synpcc7942_2427 0.75 -1.5 2,499,657 - Synpcc7942_2427 0.81 +1.9 2,499,657 - Synpcc7942_2427 0.81 +0.9
Or see this region's nucleotide sequence