Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pilT and Synpcc7942_2350 are separated by 12 nucleotides Synpcc7942_2350 and Synpcc7942_2351 are separated by 15 nucleotides Synpcc7942_2351 and lrtA are separated by 56 nucleotides lrtA and lipB are separated by 205 nucleotides
Synpcc7942_2349: pilT - twitching motility protein, at 2,416,387 to 2,417,580
pilT
Synpcc7942_2350: Synpcc7942_2350 - translation factor SUA5, at 2,417,593 to 2,418,294
_2350
Synpcc7942_2351: Synpcc7942_2351 - hypothetical protein, at 2,418,310 to 2,419,518
_2351
Synpcc7942_2352: lrtA - sigma 54 modulation protein / SSU ribosomal protein S30P, at 2,419,575 to 2,420,198
lrtA
Synpcc7942_2353: lipB - lipoyltransferase, at 2,420,404 to 2,421,105
lipB
Position (kb)
2418
2419
2420 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2417.311 kb on - strand, within pilT at 2417.386 kb on + strand, within pilT at 2417.526 kb on + strand at 2417.540 kb on + strand at 2417.562 kb on + strand at 2417.574 kb on + strand at 2417.590 kb on + strand at 2417.598 kb on - strand at 2417.619 kb on + strand at 2417.627 kb on - strand at 2417.627 kb on - strand at 2417.627 kb on - strand at 2417.642 kb on + strand at 2417.643 kb on - strand at 2417.660 kb on - strand at 2417.677 kb on + strand, within Synpcc7942_2350 at 2417.677 kb on + strand, within Synpcc7942_2350 at 2417.685 kb on - strand, within Synpcc7942_2350 at 2417.687 kb on + strand, within Synpcc7942_2350 at 2417.706 kb on + strand, within Synpcc7942_2350 at 2417.774 kb on + strand, within Synpcc7942_2350 at 2417.775 kb on + strand, within Synpcc7942_2350 at 2417.782 kb on - strand, within Synpcc7942_2350 at 2417.824 kb on + strand, within Synpcc7942_2350 at 2417.832 kb on - strand, within Synpcc7942_2350 at 2417.832 kb on - strand, within Synpcc7942_2350 at 2417.859 kb on - strand, within Synpcc7942_2350 at 2417.984 kb on - strand, within Synpcc7942_2350 at 2418.002 kb on + strand, within Synpcc7942_2350 at 2418.002 kb on + strand, within Synpcc7942_2350 at 2418.002 kb on - strand, within Synpcc7942_2350 at 2418.010 kb on - strand, within Synpcc7942_2350 at 2418.058 kb on + strand, within Synpcc7942_2350 at 2418.066 kb on - strand, within Synpcc7942_2350 at 2418.098 kb on - strand, within Synpcc7942_2350 at 2418.098 kb on - strand, within Synpcc7942_2350 at 2418.100 kb on - strand, within Synpcc7942_2350 at 2418.131 kb on - strand, within Synpcc7942_2350 at 2418.140 kb on - strand, within Synpcc7942_2350 at 2418.140 kb on - strand, within Synpcc7942_2350 at 2418.142 kb on + strand, within Synpcc7942_2350 at 2418.142 kb on + strand, within Synpcc7942_2350 at 2418.157 kb on + strand, within Synpcc7942_2350 at 2418.165 kb on - strand, within Synpcc7942_2350 at 2418.192 kb on - strand, within Synpcc7942_2350 at 2418.202 kb on + strand, within Synpcc7942_2350 at 2418.202 kb on - strand, within Synpcc7942_2350 at 2418.233 kb on - strand at 2418.233 kb on - strand at 2418.247 kb on + strand at 2418.255 kb on - strand at 2418.263 kb on - strand at 2418.263 kb on - strand at 2418.265 kb on + strand at 2418.265 kb on + strand at 2418.265 kb on + strand at 2418.273 kb on - strand at 2418.291 kb on + strand at 2418.291 kb on + strand at 2418.306 kb on - strand at 2418.714 kb on - strand, within Synpcc7942_2351 at 2419.052 kb on - strand, within Synpcc7942_2351 at 2419.053 kb on + strand, within Synpcc7942_2351 at 2419.118 kb on + strand, within Synpcc7942_2351 at 2419.556 kb on - strand at 2419.573 kb on + strand at 2419.593 kb on - strand at 2419.593 kb on - strand at 2419.723 kb on - strand, within lrtA at 2419.729 kb on + strand, within lrtA at 2419.766 kb on - strand, within lrtA at 2419.767 kb on - strand, within lrtA at 2419.879 kb on + strand, within lrtA at 2419.879 kb on + strand, within lrtA at 2420.231 kb on + strand at 2420.309 kb on + strand at 2420.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 2,417,311 - pilT Synpcc7942_2349 0.77 +0.3 2,417,386 + pilT Synpcc7942_2349 0.84 -1.1 2,417,526 + +0.4 2,417,540 + +1.4 2,417,562 + -0.5 2,417,574 + -2.4 2,417,590 + -0.6 2,417,598 - +2.7 2,417,619 + +0.3 2,417,627 - +0.5 2,417,627 - -2.1 2,417,627 - -1.1 2,417,642 + -0.6 2,417,643 - +0.5 2,417,660 - +1.0 2,417,677 + Synpcc7942_2350 0.12 +1.9 2,417,677 + Synpcc7942_2350 0.12 +0.4 2,417,685 - Synpcc7942_2350 0.13 +0.3 2,417,687 + Synpcc7942_2350 0.13 -0.7 2,417,706 + Synpcc7942_2350 0.16 -0.4 2,417,774 + Synpcc7942_2350 0.26 -0.1 2,417,775 + Synpcc7942_2350 0.26 -1.1 2,417,782 - Synpcc7942_2350 0.27 +0.9 2,417,824 + Synpcc7942_2350 0.33 +0.3 2,417,832 - Synpcc7942_2350 0.34 -0.9 2,417,832 - Synpcc7942_2350 0.34 +0.9 2,417,859 - Synpcc7942_2350 0.38 +1.5 2,417,984 - Synpcc7942_2350 0.56 -0.7 2,418,002 + Synpcc7942_2350 0.58 -1.1 2,418,002 + Synpcc7942_2350 0.58 -0.4 2,418,002 - Synpcc7942_2350 0.58 +1.8 2,418,010 - Synpcc7942_2350 0.59 +1.5 2,418,058 + Synpcc7942_2350 0.66 +0.9 2,418,066 - Synpcc7942_2350 0.67 -2.4 2,418,098 - Synpcc7942_2350 0.72 -0.0 2,418,098 - Synpcc7942_2350 0.72 -0.5 2,418,100 - Synpcc7942_2350 0.72 -0.1 2,418,131 - Synpcc7942_2350 0.77 -0.3 2,418,140 - Synpcc7942_2350 0.78 -3.4 2,418,140 - Synpcc7942_2350 0.78 -1.3 2,418,142 + Synpcc7942_2350 0.78 -1.1 2,418,142 + Synpcc7942_2350 0.78 +0.3 2,418,157 + Synpcc7942_2350 0.80 -0.0 2,418,165 - Synpcc7942_2350 0.81 -0.3 2,418,192 - Synpcc7942_2350 0.85 +0.9 2,418,202 + Synpcc7942_2350 0.87 -0.2 2,418,202 - Synpcc7942_2350 0.87 -1.2 2,418,233 - -0.5 2,418,233 - -3.1 2,418,247 + +1.9 2,418,255 - -0.7 2,418,263 - +1.9 2,418,263 - -1.1 2,418,265 + +0.9 2,418,265 + +1.5 2,418,265 + +0.4 2,418,273 - +1.5 2,418,291 + +0.9 2,418,291 + +0.5 2,418,306 - -0.8 2,418,714 - Synpcc7942_2351 0.33 -0.6 2,419,052 - Synpcc7942_2351 0.61 -0.7 2,419,053 + Synpcc7942_2351 0.61 -2.9 2,419,118 + Synpcc7942_2351 0.67 -1.4 2,419,556 - -0.4 2,419,573 + -0.8 2,419,593 - +0.3 2,419,593 - -0.5 2,419,723 - lrtA Synpcc7942_2352 0.24 -0.9 2,419,729 + lrtA Synpcc7942_2352 0.25 -1.3 2,419,766 - lrtA Synpcc7942_2352 0.31 -0.1 2,419,767 - lrtA Synpcc7942_2352 0.31 +1.1 2,419,879 + lrtA Synpcc7942_2352 0.49 +1.3 2,419,879 + lrtA Synpcc7942_2352 0.49 +0.4 2,420,231 + -0.2 2,420,309 + -0.4 2,420,386 + +0.4
Or see this region's nucleotide sequence