Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Gly and Synpcc7942_1565 are separated by 100 nucleotides Synpcc7942_1565 and ppk are separated by 38 nucleotides ppk and Synpcc7942_1567 are separated by 57 nucleotides Synpcc7942_1567 and Synpcc7942_1568 are separated by 337 nucleotides
Synpcc7942_R0001: tRNA-Gly - tRNA-Gly, at 1,622,798 to 1,622,869
tRNA-Gly
Synpcc7942_1565: Synpcc7942_1565 - hypothetical protein, at 1,622,970 to 1,623,410
_1565
Synpcc7942_1566: ppk - polyphosphate kinase, at 1,623,449 to 1,625,644
ppk
Synpcc7942_1567: Synpcc7942_1567 - possible ribosomal protein L36, at 1,625,702 to 1,626,019
_1567
Synpcc7942_1568: Synpcc7942_1568 - hypothetical protein, at 1,626,357 to 1,626,596
_1568
Position (kb) 1623
1624
1625
1626 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 1622.468 kb on + strand at 1622.477 kb on - strand at 1622.869 kb on + strand at 1622.877 kb on - strand at 1622.919 kb on + strand at 1622.919 kb on + strand at 1622.921 kb on + strand at 1622.931 kb on + strand at 1622.950 kb on - strand at 1622.960 kb on - strand at 1622.966 kb on - strand at 1622.987 kb on - strand at 1622.991 kb on + strand at 1622.999 kb on - strand at 1623.038 kb on + strand, within Synpcc7942_1565 at 1623.102 kb on + strand, within Synpcc7942_1565 at 1623.114 kb on - strand, within Synpcc7942_1565 at 1623.146 kb on - strand, within Synpcc7942_1565 at 1623.255 kb on + strand, within Synpcc7942_1565 at 1623.265 kb on - strand, within Synpcc7942_1565 at 1623.270 kb on - strand, within Synpcc7942_1565 at 1623.329 kb on - strand, within Synpcc7942_1565 at 1623.344 kb on - strand, within Synpcc7942_1565 at 1623.348 kb on + strand, within Synpcc7942_1565 at 1623.372 kb on - strand at 1623.401 kb on + strand at 1623.406 kb on + strand at 1623.411 kb on + strand at 1623.415 kb on + strand at 1623.415 kb on + strand at 1623.423 kb on - strand at 1623.446 kb on + strand at 1623.457 kb on - strand at 1623.493 kb on + strand at 1623.591 kb on + strand at 1623.622 kb on + strand at 1623.651 kb on + strand at 1623.667 kb on - strand at 1623.736 kb on + strand, within ppk at 1623.771 kb on + strand, within ppk at 1623.810 kb on + strand, within ppk at 1623.815 kb on + strand, within ppk at 1623.871 kb on + strand, within ppk at 1623.901 kb on + strand, within ppk at 1623.950 kb on - strand, within ppk at 1623.972 kb on + strand, within ppk at 1623.991 kb on - strand, within ppk at 1624.025 kb on - strand, within ppk at 1624.262 kb on - strand, within ppk at 1624.275 kb on + strand, within ppk at 1624.305 kb on - strand, within ppk at 1624.310 kb on - strand, within ppk at 1624.354 kb on - strand, within ppk at 1624.381 kb on - strand, within ppk at 1624.397 kb on + strand, within ppk at 1624.543 kb on - strand, within ppk at 1624.545 kb on + strand, within ppk at 1624.553 kb on - strand, within ppk at 1624.553 kb on - strand, within ppk at 1624.553 kb on - strand, within ppk at 1624.560 kb on - strand at 1624.560 kb on - strand, within ppk at 1624.575 kb on + strand, within ppk at 1624.611 kb on - strand, within ppk at 1624.623 kb on - strand, within ppk at 1624.655 kb on + strand, within ppk at 1624.656 kb on - strand, within ppk at 1624.710 kb on - strand, within ppk at 1624.737 kb on + strand, within ppk at 1624.794 kb on - strand, within ppk at 1624.802 kb on + strand, within ppk at 1624.824 kb on - strand, within ppk at 1624.859 kb on + strand, within ppk at 1624.867 kb on - strand, within ppk at 1624.867 kb on - strand, within ppk at 1624.874 kb on - strand, within ppk at 1624.896 kb on - strand, within ppk at 1624.898 kb on + strand, within ppk at 1624.940 kb on + strand, within ppk at 1624.974 kb on - strand, within ppk at 1624.989 kb on - strand, within ppk at 1624.991 kb on + strand, within ppk at 1624.999 kb on - strand, within ppk at 1625.117 kb on - strand at 1625.229 kb on - strand, within ppk at 1625.238 kb on - strand, within ppk at 1625.240 kb on + strand, within ppk at 1625.286 kb on + strand, within ppk at 1625.286 kb on - strand, within ppk at 1625.319 kb on - strand, within ppk at 1625.428 kb on + strand at 1625.429 kb on - strand at 1625.433 kb on + strand at 1625.434 kb on - strand at 1625.492 kb on - strand at 1625.550 kb on + strand at 1625.553 kb on + strand at 1625.556 kb on - strand at 1625.563 kb on - strand at 1625.602 kb on - strand at 1625.630 kb on + strand at 1625.638 kb on - strand at 1625.651 kb on + strand at 1625.656 kb on + strand at 1625.656 kb on + strand at 1625.664 kb on - strand at 1625.672 kb on - strand at 1625.680 kb on + strand at 1625.771 kb on - strand, within Synpcc7942_1567 at 1625.795 kb on - strand, within Synpcc7942_1567 at 1625.821 kb on + strand, within Synpcc7942_1567 at 1625.825 kb on + strand, within Synpcc7942_1567 at 1625.842 kb on + strand, within Synpcc7942_1567 at 1625.850 kb on - strand, within Synpcc7942_1567 at 1625.850 kb on - strand, within Synpcc7942_1567 at 1625.968 kb on - strand, within Synpcc7942_1567 at 1626.019 kb on - strand at 1626.049 kb on + strand at 1626.091 kb on - strand at 1626.091 kb on - strand at 1626.092 kb on + strand at 1626.092 kb on + strand at 1626.092 kb on + strand at 1626.100 kb on - strand at 1626.100 kb on - strand at 1626.100 kb on - strand at 1626.100 kb on - strand at 1626.135 kb on - strand at 1626.135 kb on - strand at 1626.152 kb on + strand at 1626.160 kb on - strand at 1626.160 kb on - strand at 1626.179 kb on - strand at 1626.204 kb on + strand at 1626.227 kb on - strand at 1626.251 kb on + strand at 1626.251 kb on + strand at 1626.259 kb on - strand at 1626.264 kb on - strand at 1626.276 kb on + strand at 1626.305 kb on + strand at 1626.318 kb on - strand at 1626.323 kb on + strand at 1626.366 kb on - strand at 1626.391 kb on + strand, within Synpcc7942_1568 at 1626.391 kb on + strand, within Synpcc7942_1568 at 1626.394 kb on - strand, within Synpcc7942_1568 at 1626.399 kb on - strand, within Synpcc7942_1568 at 1626.399 kb on - strand, within Synpcc7942_1568 at 1626.403 kb on - strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.518 kb on + strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.526 kb on - strand, within Synpcc7942_1568 at 1626.607 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 1,622,468 + +0.0 1,622,477 - +2.4 1,622,869 + -0.8 1,622,877 - +0.9 1,622,919 + -0.1 1,622,919 + -0.3 1,622,921 + -0.5 1,622,931 + +0.8 1,622,950 - -1.3 1,622,960 - -2.1 1,622,966 - +0.2 1,622,987 - +0.2 1,622,991 + +0.8 1,622,999 - -1.8 1,623,038 + Synpcc7942_1565 0.15 -1.1 1,623,102 + Synpcc7942_1565 0.30 +1.9 1,623,114 - Synpcc7942_1565 0.33 -3.1 1,623,146 - Synpcc7942_1565 0.40 -2.6 1,623,255 + Synpcc7942_1565 0.65 +1.5 1,623,265 - Synpcc7942_1565 0.67 -0.1 1,623,270 - Synpcc7942_1565 0.68 -2.6 1,623,329 - Synpcc7942_1565 0.81 +0.9 1,623,344 - Synpcc7942_1565 0.85 -0.9 1,623,348 + Synpcc7942_1565 0.86 -0.1 1,623,372 - -1.6 1,623,401 + -0.1 1,623,406 + -0.1 1,623,411 + -1.1 1,623,415 + -0.1 1,623,415 + -0.8 1,623,423 - -0.6 1,623,446 + +0.2 1,623,457 - -0.1 1,623,493 + -0.6 1,623,591 + -1.9 1,623,622 + -2.6 1,623,651 + -1.6 1,623,667 - -2.2 1,623,736 + ppk Synpcc7942_1566 0.13 -3.2 1,623,771 + ppk Synpcc7942_1566 0.15 -2.4 1,623,810 + ppk Synpcc7942_1566 0.16 -1.6 1,623,815 + ppk Synpcc7942_1566 0.17 -4.1 1,623,871 + ppk Synpcc7942_1566 0.19 -1.1 1,623,901 + ppk Synpcc7942_1566 0.21 -2.4 1,623,950 - ppk Synpcc7942_1566 0.23 -2.1 1,623,972 + ppk Synpcc7942_1566 0.24 -3.1 1,623,991 - ppk Synpcc7942_1566 0.25 -0.1 1,624,025 - ppk Synpcc7942_1566 0.26 -1.6 1,624,262 - ppk Synpcc7942_1566 0.37 -5.0 1,624,275 + ppk Synpcc7942_1566 0.38 -4.9 1,624,305 - ppk Synpcc7942_1566 0.39 -4.9 1,624,310 - ppk Synpcc7942_1566 0.39 -1.1 1,624,354 - ppk Synpcc7942_1566 0.41 -2.9 1,624,381 - ppk Synpcc7942_1566 0.42 -2.1 1,624,397 + ppk Synpcc7942_1566 0.43 -3.1 1,624,543 - ppk Synpcc7942_1566 0.50 -0.3 1,624,545 + ppk Synpcc7942_1566 0.50 -2.9 1,624,553 - ppk Synpcc7942_1566 0.50 -4.5 1,624,553 - ppk Synpcc7942_1566 0.50 -1.1 1,624,553 - ppk Synpcc7942_1566 0.50 -3.1 1,624,560 - -1.4 1,624,560 - ppk Synpcc7942_1566 0.51 -3.1 1,624,575 + ppk Synpcc7942_1566 0.51 -1.6 1,624,611 - ppk Synpcc7942_1566 0.53 -3.2 1,624,623 - ppk Synpcc7942_1566 0.53 -2.2 1,624,655 + ppk Synpcc7942_1566 0.55 -2.6 1,624,656 - ppk Synpcc7942_1566 0.55 -2.9 1,624,710 - ppk Synpcc7942_1566 0.57 -1.1 1,624,737 + ppk Synpcc7942_1566 0.59 -2.1 1,624,794 - ppk Synpcc7942_1566 0.61 -1.1 1,624,802 + ppk Synpcc7942_1566 0.62 -2.4 1,624,824 - ppk Synpcc7942_1566 0.63 -1.1 1,624,859 + ppk Synpcc7942_1566 0.64 -1.6 1,624,867 - ppk Synpcc7942_1566 0.65 -3.1 1,624,867 - ppk Synpcc7942_1566 0.65 -3.4 1,624,874 - ppk Synpcc7942_1566 0.65 -3.6 1,624,896 - ppk Synpcc7942_1566 0.66 -3.8 1,624,898 + ppk Synpcc7942_1566 0.66 -3.2 1,624,940 + ppk Synpcc7942_1566 0.68 -4.3 1,624,974 - ppk Synpcc7942_1566 0.69 -2.9 1,624,989 - ppk Synpcc7942_1566 0.70 -0.1 1,624,991 + ppk Synpcc7942_1566 0.70 -2.6 1,624,999 - ppk Synpcc7942_1566 0.71 -2.1 1,625,117 - -1.1 1,625,229 - ppk Synpcc7942_1566 0.81 -2.0 1,625,238 - ppk Synpcc7942_1566 0.81 -3.5 1,625,240 + ppk Synpcc7942_1566 0.82 -2.9 1,625,286 + ppk Synpcc7942_1566 0.84 -0.3 1,625,286 - ppk Synpcc7942_1566 0.84 -2.1 1,625,319 - ppk Synpcc7942_1566 0.85 -2.6 1,625,428 + -0.4 1,625,429 - -1.1 1,625,433 + -2.9 1,625,434 - -1.1 1,625,492 - -0.1 1,625,550 + -1.1 1,625,553 + -2.1 1,625,556 - -3.2 1,625,563 - -1.3 1,625,602 - -2.1 1,625,630 + -1.4 1,625,638 - +0.6 1,625,651 + +1.2 1,625,656 + +0.2 1,625,656 + +0.5 1,625,664 - -2.4 1,625,672 - -0.1 1,625,680 + +0.9 1,625,771 - Synpcc7942_1567 0.22 +0.7 1,625,795 - Synpcc7942_1567 0.29 +0.2 1,625,821 + Synpcc7942_1567 0.37 -2.8 1,625,825 + Synpcc7942_1567 0.39 -1.6 1,625,842 + Synpcc7942_1567 0.44 -0.3 1,625,850 - Synpcc7942_1567 0.47 +0.5 1,625,850 - Synpcc7942_1567 0.47 -0.5 1,625,968 - Synpcc7942_1567 0.84 +0.4 1,626,019 - +0.4 1,626,049 + +0.5 1,626,091 - +0.9 1,626,091 - -0.3 1,626,092 + +1.2 1,626,092 + +0.9 1,626,092 + -0.4 1,626,100 - -1.1 1,626,100 - -1.8 1,626,100 - +1.5 1,626,100 - -0.3 1,626,135 - +0.8 1,626,135 - -1.3 1,626,152 + +0.2 1,626,160 - +0.2 1,626,160 - -1.6 1,626,179 - -0.1 1,626,204 + -1.1 1,626,227 - +0.2 1,626,251 + -1.6 1,626,251 + -0.1 1,626,259 - +0.9 1,626,264 - +0.4 1,626,276 + +0.5 1,626,305 + +1.1 1,626,318 - -0.9 1,626,323 + -0.1 1,626,366 - +1.5 1,626,391 + Synpcc7942_1568 0.14 -0.6 1,626,391 + Synpcc7942_1568 0.14 -0.4 1,626,394 - Synpcc7942_1568 0.15 -0.5 1,626,399 - Synpcc7942_1568 0.17 +0.5 1,626,399 - Synpcc7942_1568 0.17 +0.5 1,626,403 - Synpcc7942_1568 0.19 +0.2 1,626,518 + Synpcc7942_1568 0.67 -0.4 1,626,518 + Synpcc7942_1568 0.67 -0.1 1,626,518 + Synpcc7942_1568 0.67 +0.3 1,626,518 + Synpcc7942_1568 0.67 -0.4 1,626,518 + Synpcc7942_1568 0.67 -1.6 1,626,518 + Synpcc7942_1568 0.67 -1.6 1,626,518 + Synpcc7942_1568 0.67 +0.5 1,626,518 + Synpcc7942_1568 0.67 -1.9 1,626,518 + Synpcc7942_1568 0.67 -1.1 1,626,518 + Synpcc7942_1568 0.67 -1.3 1,626,526 - Synpcc7942_1568 0.70 +0.2 1,626,526 - Synpcc7942_1568 0.70 -0.7 1,626,526 - Synpcc7942_1568 0.70 -1.1 1,626,526 - Synpcc7942_1568 0.70 +1.4 1,626,526 - Synpcc7942_1568 0.70 -0.5 1,626,526 - Synpcc7942_1568 0.70 -0.1 1,626,526 - Synpcc7942_1568 0.70 -1.2 1,626,526 - Synpcc7942_1568 0.70 +0.4 1,626,526 - Synpcc7942_1568 0.70 +1.2 1,626,526 - Synpcc7942_1568 0.70 -0.1 1,626,526 - Synpcc7942_1568 0.70 +0.1 1,626,526 - Synpcc7942_1568 0.70 +0.2 1,626,526 - Synpcc7942_1568 0.70 +0.1 1,626,607 + +0.2
Or see this region's nucleotide sequence