Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiE and ycf40 are separated by 17 nucleotides ycf40 and Synpcc7942_1059 are separated by 112 nucleotides Synpcc7942_1059 and Synpcc7942_1060 are separated by 191 nucleotides
Synpcc7942_1057: thiE - thiamine-phosphate pyrophosphorylase, at 1,067,527 to 1,068,558
thiE
Synpcc7942_1058: ycf40 - hypothetical protein, at 1,068,576 to 1,068,788
ycf40
Synpcc7942_1059: Synpcc7942_1059 - hypothetical protein, at 1,068,901 to 1,069,833
_1059
Synpcc7942_1060: Synpcc7942_1060 - type I restriction-modification, at 1,070,025 to 1,072,052
_1060
Position (kb)
1068
1069
1070 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1067.925 kb on - strand, within thiE at 1068.566 kb on + strand at 1068.566 kb on + strand at 1068.574 kb on - strand at 1068.792 kb on - strand at 1068.795 kb on - strand at 1068.801 kb on + strand at 1068.815 kb on - strand at 1068.824 kb on + strand at 1068.850 kb on + strand at 1068.852 kb on + strand at 1068.852 kb on + strand at 1068.860 kb on - strand at 1068.891 kb on + strand at 1068.891 kb on + strand at 1068.891 kb on + strand at 1068.891 kb on + strand at 1068.891 kb on + strand at 1068.899 kb on - strand at 1068.899 kb on - strand at 1068.965 kb on - strand at 1068.969 kb on + strand at 1069.128 kb on - strand, within Synpcc7942_1059 at 1069.233 kb on + strand, within Synpcc7942_1059 at 1069.361 kb on - strand, within Synpcc7942_1059 at 1069.394 kb on - strand, within Synpcc7942_1059 at 1069.431 kb on - strand, within Synpcc7942_1059 at 1069.443 kb on - strand, within Synpcc7942_1059 at 1069.443 kb on - strand, within Synpcc7942_1059 at 1069.448 kb on - strand, within Synpcc7942_1059 at 1069.486 kb on + strand, within Synpcc7942_1059 at 1069.486 kb on + strand, within Synpcc7942_1059 at 1069.502 kb on + strand, within Synpcc7942_1059 at 1069.510 kb on - strand, within Synpcc7942_1059 at 1069.572 kb on + strand, within Synpcc7942_1059 at 1069.654 kb on + strand, within Synpcc7942_1059 at 1069.677 kb on - strand, within Synpcc7942_1059 at 1069.677 kb on - strand, within Synpcc7942_1059 at 1069.677 kb on - strand, within Synpcc7942_1059 at 1069.742 kb on + strand at 1069.750 kb on + strand at 1069.806 kb on + strand at 1069.828 kb on + strand at 1069.836 kb on - strand at 1069.836 kb on - strand at 1069.847 kb on + strand at 1069.855 kb on - strand at 1069.872 kb on + strand at 1069.872 kb on + strand at 1069.874 kb on + strand at 1069.880 kb on - strand at 1069.880 kb on - strand at 1069.940 kb on + strand at 1069.975 kb on + strand at 1069.983 kb on - strand at 1069.997 kb on - strand at 1069.997 kb on - strand at 1069.999 kb on + strand at 1070.008 kb on + strand at 1070.008 kb on - strand at 1070.065 kb on - strand at 1070.088 kb on - strand at 1070.089 kb on - strand at 1070.089 kb on - strand at 1070.090 kb on + strand at 1070.098 kb on - strand at 1070.143 kb on - strand at 1070.147 kb on + strand at 1070.176 kb on - strand at 1070.181 kb on - strand at 1070.216 kb on - strand at 1070.234 kb on - strand, within Synpcc7942_1060 at 1070.245 kb on - strand, within Synpcc7942_1060 at 1070.279 kb on + strand, within Synpcc7942_1060 at 1070.301 kb on - strand, within Synpcc7942_1060 at 1070.325 kb on + strand, within Synpcc7942_1060 at 1070.325 kb on + strand, within Synpcc7942_1060 at 1070.347 kb on + strand, within Synpcc7942_1060 at 1070.405 kb on + strand, within Synpcc7942_1060 at 1070.420 kb on - strand, within Synpcc7942_1060 at 1070.420 kb on - strand, within Synpcc7942_1060 at 1070.428 kb on + strand, within Synpcc7942_1060 at 1070.484 kb on + strand, within Synpcc7942_1060 at 1070.484 kb on + strand, within Synpcc7942_1060 at 1070.490 kb on + strand, within Synpcc7942_1060 at 1070.505 kb on - strand, within Synpcc7942_1060 at 1070.521 kb on + strand, within Synpcc7942_1060 at 1070.524 kb on - strand, within Synpcc7942_1060 at 1070.557 kb on + strand, within Synpcc7942_1060 at 1070.588 kb on - strand, within Synpcc7942_1060 at 1070.596 kb on - strand, within Synpcc7942_1060 at 1070.601 kb on - strand at 1070.621 kb on - strand, within Synpcc7942_1060 at 1070.661 kb on - strand, within Synpcc7942_1060 at 1070.681 kb on + strand, within Synpcc7942_1060 at 1070.697 kb on + strand, within Synpcc7942_1060 at 1070.699 kb on + strand, within Synpcc7942_1060 at 1070.707 kb on - strand, within Synpcc7942_1060 at 1070.821 kb on + strand, within Synpcc7942_1060
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 1,067,925 - thiE Synpcc7942_1057 0.39 -0.8 1,068,566 + +0.5 1,068,566 + +1.1 1,068,574 - -0.1 1,068,792 - +0.2 1,068,795 - +0.9 1,068,801 + -0.6 1,068,815 - +1.6 1,068,824 + -1.4 1,068,850 + -1.3 1,068,852 + +0.3 1,068,852 + -1.4 1,068,860 - +0.9 1,068,891 + -0.8 1,068,891 + -0.1 1,068,891 + -0.1 1,068,891 + +0.2 1,068,891 + +0.4 1,068,899 - +0.7 1,068,899 - +0.8 1,068,965 - -2.7 1,068,969 + -4.1 1,069,128 - Synpcc7942_1059 0.24 -2.7 1,069,233 + Synpcc7942_1059 0.36 -3.8 1,069,361 - Synpcc7942_1059 0.49 -0.1 1,069,394 - Synpcc7942_1059 0.53 -1.3 1,069,431 - Synpcc7942_1059 0.57 -0.1 1,069,443 - Synpcc7942_1059 0.58 -2.4 1,069,443 - Synpcc7942_1059 0.58 -2.4 1,069,448 - Synpcc7942_1059 0.59 -3.3 1,069,486 + Synpcc7942_1059 0.63 -1.7 1,069,486 + Synpcc7942_1059 0.63 -2.1 1,069,502 + Synpcc7942_1059 0.64 +0.9 1,069,510 - Synpcc7942_1059 0.65 -1.1 1,069,572 + Synpcc7942_1059 0.72 -0.7 1,069,654 + Synpcc7942_1059 0.81 -1.2 1,069,677 - Synpcc7942_1059 0.83 -1.1 1,069,677 - Synpcc7942_1059 0.83 -2.1 1,069,677 - Synpcc7942_1059 0.83 -1.1 1,069,742 + -3.8 1,069,750 + -0.1 1,069,806 + -1.9 1,069,828 + +3.0 1,069,836 - +0.9 1,069,836 - +0.2 1,069,847 + -0.1 1,069,855 - +1.2 1,069,872 + +0.9 1,069,872 + +0.3 1,069,874 + -0.1 1,069,880 - -0.2 1,069,880 - -1.3 1,069,940 + +1.0 1,069,975 + -0.6 1,069,983 - -1.4 1,069,997 - +1.4 1,069,997 - -0.7 1,069,999 + +0.9 1,070,008 + -0.4 1,070,008 - +0.3 1,070,065 - -0.6 1,070,088 - +0.2 1,070,089 - +1.5 1,070,089 - +0.7 1,070,090 + +0.7 1,070,098 - +0.6 1,070,143 - +0.2 1,070,147 + -1.7 1,070,176 - +0.6 1,070,181 - +0.9 1,070,216 - -2.4 1,070,234 - Synpcc7942_1060 0.10 -0.1 1,070,245 - Synpcc7942_1060 0.11 -0.6 1,070,279 + Synpcc7942_1060 0.13 +0.3 1,070,301 - Synpcc7942_1060 0.14 -0.3 1,070,325 + Synpcc7942_1060 0.15 +0.9 1,070,325 + Synpcc7942_1060 0.15 +0.5 1,070,347 + Synpcc7942_1060 0.16 -1.0 1,070,405 + Synpcc7942_1060 0.19 -0.5 1,070,420 - Synpcc7942_1060 0.19 +0.1 1,070,420 - Synpcc7942_1060 0.19 +0.6 1,070,428 + Synpcc7942_1060 0.20 +0.3 1,070,484 + Synpcc7942_1060 0.23 +0.1 1,070,484 + Synpcc7942_1060 0.23 +1.1 1,070,490 + Synpcc7942_1060 0.23 +0.9 1,070,505 - Synpcc7942_1060 0.24 +0.2 1,070,521 + Synpcc7942_1060 0.24 -1.1 1,070,524 - Synpcc7942_1060 0.25 -1.1 1,070,557 + Synpcc7942_1060 0.26 -0.6 1,070,588 - Synpcc7942_1060 0.28 -0.9 1,070,596 - Synpcc7942_1060 0.28 -0.3 1,070,601 - +2.2 1,070,621 - Synpcc7942_1060 0.29 -1.4 1,070,661 - Synpcc7942_1060 0.31 +1.2 1,070,681 + Synpcc7942_1060 0.32 +0.1 1,070,697 + Synpcc7942_1060 0.33 +0.2 1,070,699 + Synpcc7942_1060 0.33 -0.1 1,070,707 - Synpcc7942_1060 0.34 +2.1 1,070,821 + Synpcc7942_1060 0.39 +0.9
Or see this region's nucleotide sequence