Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpcA2 and cpcE are separated by 90 nucleotides cpcE and cpcF are separated by 6 nucleotides cpcF and Synpcc7942_1056 are separated by 26 nucleotides Synpcc7942_1056 and thiE are separated by 38 nucleotides
Synpcc7942_1053: cpcA2 - phycocyanin, alpha subunit, at 1,064,646 to 1,065,137
cpcA2
Synpcc7942_1054: cpcE - PBS lyase HEAT-like repeat, at 1,065,228 to 1,066,049
cpcE
Synpcc7942_1055: cpcF - phycocyanin alpha-subunit phycocyanobilin lyase, at 1,066,056 to 1,066,679
cpcF
Synpcc7942_1056: Synpcc7942_1056 - transcriptional regulator, XRE family, at 1,066,706 to 1,067,488
_1056
Synpcc7942_1057: thiE - thiamine-phosphate pyrophosphorylase, at 1,067,527 to 1,068,558
thiE
Position (kb)
1066
1067 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1065.101 kb on + strand at 1065.137 kb on + strand at 1065.145 kb on - strand at 1065.145 kb on - strand at 1065.155 kb on + strand at 1065.155 kb on + strand at 1065.160 kb on + strand at 1065.161 kb on + strand at 1065.161 kb on + strand at 1065.170 kb on + strand at 1065.199 kb on - strand at 1065.411 kb on + strand, within cpcE at 1065.702 kb on + strand, within cpcE at 1066.053 kb on + strand at 1066.067 kb on + strand at 1066.073 kb on - strand at 1066.075 kb on - strand at 1066.300 kb on - strand, within cpcF at 1066.302 kb on - strand, within cpcF at 1066.406 kb on - strand, within cpcF at 1066.477 kb on + strand, within cpcF at 1066.682 kb on + strand at 1066.702 kb on - strand at 1066.720 kb on + strand at 1066.730 kb on + strand at 1066.733 kb on + strand at 1066.746 kb on - strand at 1066.757 kb on - strand at 1066.775 kb on + strand at 1066.805 kb on + strand, within Synpcc7942_1056 at 1066.805 kb on + strand, within Synpcc7942_1056 at 1066.805 kb on + strand, within Synpcc7942_1056 at 1066.813 kb on - strand, within Synpcc7942_1056 at 1066.828 kb on - strand, within Synpcc7942_1056 at 1066.829 kb on - strand, within Synpcc7942_1056 at 1066.830 kb on + strand, within Synpcc7942_1056 at 1066.838 kb on - strand, within Synpcc7942_1056 at 1066.856 kb on + strand, within Synpcc7942_1056 at 1066.856 kb on + strand, within Synpcc7942_1056 at 1066.863 kb on - strand, within Synpcc7942_1056 at 1066.875 kb on - strand, within Synpcc7942_1056 at 1066.888 kb on + strand, within Synpcc7942_1056 at 1066.898 kb on - strand, within Synpcc7942_1056 at 1066.910 kb on - strand, within Synpcc7942_1056 at 1066.962 kb on + strand, within Synpcc7942_1056 at 1066.972 kb on + strand, within Synpcc7942_1056 at 1066.988 kb on - strand, within Synpcc7942_1056 at 1067.032 kb on + strand, within Synpcc7942_1056 at 1067.032 kb on - strand, within Synpcc7942_1056 at 1067.051 kb on - strand, within Synpcc7942_1056 at 1067.058 kb on + strand, within Synpcc7942_1056 at 1067.071 kb on + strand, within Synpcc7942_1056 at 1067.103 kb on - strand, within Synpcc7942_1056 at 1067.111 kb on - strand, within Synpcc7942_1056 at 1067.130 kb on + strand, within Synpcc7942_1056 at 1067.138 kb on - strand, within Synpcc7942_1056 at 1067.151 kb on + strand, within Synpcc7942_1056 at 1067.170 kb on + strand, within Synpcc7942_1056 at 1067.185 kb on + strand, within Synpcc7942_1056 at 1067.193 kb on - strand, within Synpcc7942_1056 at 1067.221 kb on + strand, within Synpcc7942_1056 at 1067.227 kb on - strand, within Synpcc7942_1056 at 1067.262 kb on - strand, within Synpcc7942_1056 at 1067.278 kb on - strand, within Synpcc7942_1056 at 1067.295 kb on - strand, within Synpcc7942_1056 at 1067.300 kb on + strand, within Synpcc7942_1056 at 1067.316 kb on - strand, within Synpcc7942_1056 at 1067.379 kb on - strand, within Synpcc7942_1056 at 1067.390 kb on + strand, within Synpcc7942_1056 at 1067.416 kb on + strand at 1067.420 kb on - strand at 1067.454 kb on + strand at 1067.461 kb on + strand at 1067.466 kb on + strand at 1067.466 kb on + strand at 1067.466 kb on + strand at 1067.469 kb on - strand at 1067.469 kb on - strand at 1067.469 kb on - strand at 1067.474 kb on - strand at 1067.497 kb on + strand at 1067.497 kb on + strand at 1067.511 kb on + strand at 1067.511 kb on + strand at 1067.511 kb on + strand at 1067.519 kb on - strand at 1067.526 kb on + strand at 1067.539 kb on - strand at 1067.539 kb on - strand at 1067.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 1,065,101 + +2.1 1,065,137 + -0.1 1,065,145 - +1.3 1,065,145 - -0.4 1,065,155 + -0.3 1,065,155 + -0.5 1,065,160 + +1.1 1,065,161 + -0.1 1,065,161 + -0.1 1,065,170 + +0.5 1,065,199 - +0.6 1,065,411 + cpcE Synpcc7942_1054 0.22 -2.7 1,065,702 + cpcE Synpcc7942_1054 0.58 -2.9 1,066,053 + -0.4 1,066,067 + +0.9 1,066,073 - +0.2 1,066,075 - +0.9 1,066,300 - cpcF Synpcc7942_1055 0.39 -0.7 1,066,302 - cpcF Synpcc7942_1055 0.39 -1.3 1,066,406 - cpcF Synpcc7942_1055 0.56 -2.4 1,066,477 + cpcF Synpcc7942_1055 0.67 -3.1 1,066,682 + +0.3 1,066,702 - +0.8 1,066,720 + +0.4 1,066,730 + +0.8 1,066,733 + -0.1 1,066,746 - -1.8 1,066,757 - -2.3 1,066,775 + -1.1 1,066,805 + Synpcc7942_1056 0.13 +0.3 1,066,805 + Synpcc7942_1056 0.13 +0.9 1,066,805 + Synpcc7942_1056 0.13 -2.7 1,066,813 - Synpcc7942_1056 0.14 +0.9 1,066,828 - Synpcc7942_1056 0.16 +1.0 1,066,829 - Synpcc7942_1056 0.16 +0.2 1,066,830 + Synpcc7942_1056 0.16 +0.1 1,066,838 - Synpcc7942_1056 0.17 +0.6 1,066,856 + Synpcc7942_1056 0.19 -1.1 1,066,856 + Synpcc7942_1056 0.19 -0.4 1,066,863 - Synpcc7942_1056 0.20 +0.9 1,066,875 - Synpcc7942_1056 0.22 -0.8 1,066,888 + Synpcc7942_1056 0.23 -1.1 1,066,898 - Synpcc7942_1056 0.25 -0.4 1,066,910 - Synpcc7942_1056 0.26 -1.4 1,066,962 + Synpcc7942_1056 0.33 +0.1 1,066,972 + Synpcc7942_1056 0.34 +0.9 1,066,988 - Synpcc7942_1056 0.36 -0.1 1,067,032 + Synpcc7942_1056 0.42 -0.3 1,067,032 - Synpcc7942_1056 0.42 +0.6 1,067,051 - Synpcc7942_1056 0.44 -0.1 1,067,058 + Synpcc7942_1056 0.45 -1.7 1,067,071 + Synpcc7942_1056 0.47 -0.8 1,067,103 - Synpcc7942_1056 0.51 +1.0 1,067,111 - Synpcc7942_1056 0.52 +0.6 1,067,130 + Synpcc7942_1056 0.54 -1.3 1,067,138 - Synpcc7942_1056 0.55 -0.1 1,067,151 + Synpcc7942_1056 0.57 +0.1 1,067,170 + Synpcc7942_1056 0.59 +0.8 1,067,185 + Synpcc7942_1056 0.61 +0.2 1,067,193 - Synpcc7942_1056 0.62 -0.4 1,067,221 + Synpcc7942_1056 0.66 -1.7 1,067,227 - Synpcc7942_1056 0.67 +0.3 1,067,262 - Synpcc7942_1056 0.71 -0.7 1,067,278 - Synpcc7942_1056 0.73 -0.1 1,067,295 - Synpcc7942_1056 0.75 -0.1 1,067,300 + Synpcc7942_1056 0.76 +0.2 1,067,316 - Synpcc7942_1056 0.78 -1.1 1,067,379 - Synpcc7942_1056 0.86 -0.2 1,067,390 + Synpcc7942_1056 0.87 -0.4 1,067,416 + +1.5 1,067,420 - -0.9 1,067,454 + -0.9 1,067,461 + -0.6 1,067,466 + -1.8 1,067,466 + -2.1 1,067,466 + +1.5 1,067,469 - -0.5 1,067,469 - -0.1 1,067,469 - +0.5 1,067,474 - +0.1 1,067,497 + -0.4 1,067,497 + +0.8 1,067,511 + +0.1 1,067,511 + +0.5 1,067,511 + +1.2 1,067,519 - +1.1 1,067,526 + +1.7 1,067,539 - -1.8 1,067,539 - -0.7 1,067,562 - -1.1
Or see this region's nucleotide sequence