Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrB and Synpcc7942_0671 are separated by 18 nucleotides Synpcc7942_0671 and rpoD3 are separated by 233 nucleotides
Synpcc7942_0670: pyrB - aspartate carbamoyltransferase catalytic subunit, at 665,121 to 666,137
pyrB
Synpcc7942_0671: Synpcc7942_0671 - hypothetical protein, at 666,156 to 666,653
_0671
Synpcc7942_0672: rpoD3 - RNA polymerase sigma factor SigD, at 666,887 to 667,849
rpoD3
Position (kb) 666
667 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 666.080 kb on + strand at 666.106 kb on + strand at 666.109 kb on - strand at 666.114 kb on - strand at 666.119 kb on - strand at 666.159 kb on + strand at 666.202 kb on + strand at 666.205 kb on - strand at 666.205 kb on - strand at 666.210 kb on - strand, within Synpcc7942_0671 at 666.229 kb on + strand, within Synpcc7942_0671 at 666.229 kb on + strand, within Synpcc7942_0671 at 666.252 kb on - strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.264 kb on - strand, within Synpcc7942_0671 at 666.264 kb on - strand, within Synpcc7942_0671 at 666.324 kb on - strand, within Synpcc7942_0671 at 666.332 kb on + strand, within Synpcc7942_0671 at 666.340 kb on - strand at 666.348 kb on - strand, within Synpcc7942_0671 at 666.377 kb on + strand, within Synpcc7942_0671 at 666.377 kb on + strand, within Synpcc7942_0671 at 666.395 kb on - strand, within Synpcc7942_0671 at 666.396 kb on - strand at 666.407 kb on + strand, within Synpcc7942_0671 at 666.407 kb on + strand, within Synpcc7942_0671 at 666.407 kb on + strand, within Synpcc7942_0671 at 666.407 kb on + strand, within Synpcc7942_0671 at 666.414 kb on + strand, within Synpcc7942_0671 at 666.415 kb on - strand, within Synpcc7942_0671 at 666.415 kb on - strand, within Synpcc7942_0671 at 666.422 kb on - strand, within Synpcc7942_0671 at 666.426 kb on + strand, within Synpcc7942_0671 at 666.431 kb on + strand, within Synpcc7942_0671 at 666.476 kb on + strand, within Synpcc7942_0671 at 666.476 kb on + strand, within Synpcc7942_0671 at 666.476 kb on + strand, within Synpcc7942_0671 at 666.484 kb on - strand, within Synpcc7942_0671 at 666.484 kb on - strand, within Synpcc7942_0671 at 666.484 kb on - strand, within Synpcc7942_0671 at 666.489 kb on - strand, within Synpcc7942_0671 at 666.497 kb on - strand, within Synpcc7942_0671 at 666.499 kb on + strand, within Synpcc7942_0671 at 666.535 kb on + strand, within Synpcc7942_0671 at 666.535 kb on + strand, within Synpcc7942_0671 at 666.538 kb on + strand, within Synpcc7942_0671 at 666.545 kb on + strand at 666.633 kb on - strand at 666.635 kb on + strand at 666.638 kb on - strand at 666.656 kb on - strand at 666.676 kb on + strand at 666.676 kb on - strand at 666.683 kb on + strand at 666.684 kb on - strand at 666.691 kb on - strand at 666.752 kb on - strand at 666.763 kb on - strand at 666.784 kb on - strand at 666.872 kb on + strand at 666.910 kb on + strand at 666.920 kb on + strand at 666.924 kb on + strand at 666.924 kb on + strand at 666.924 kb on + strand at 666.956 kb on + strand at 666.964 kb on - strand at 666.971 kb on + strand at 666.993 kb on + strand, within rpoD3 at 667.000 kb on + strand, within rpoD3 at 667.038 kb on + strand, within rpoD3 at 667.046 kb on - strand, within rpoD3 at 667.046 kb on - strand, within rpoD3 at 667.046 kb on - strand, within rpoD3 at 667.072 kb on + strand, within rpoD3 at 667.106 kb on + strand, within rpoD3 at 667.115 kb on + strand, within rpoD3 at 667.123 kb on + strand, within rpoD3 at 667.178 kb on - strand, within rpoD3 at 667.245 kb on + strand, within rpoD3 at 667.253 kb on - strand, within rpoD3 at 667.370 kb on - strand at 667.385 kb on + strand, within rpoD3 at 667.479 kb on + strand, within rpoD3 at 667.485 kb on + strand, within rpoD3 at 667.485 kb on + strand, within rpoD3 at 667.485 kb on - strand, within rpoD3 at 667.488 kb on + strand, within rpoD3 at 667.493 kb on - strand, within rpoD3
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 666,080 + +0.8 666,106 + -1.8 666,109 - +1.0 666,114 - +0.4 666,119 - +0.1 666,159 + +0.5 666,202 + +0.3 666,205 - -1.1 666,205 - -0.4 666,210 - Synpcc7942_0671 0.11 -2.4 666,229 + Synpcc7942_0671 0.15 -0.1 666,229 + Synpcc7942_0671 0.15 +1.2 666,252 - Synpcc7942_0671 0.19 +1.1 666,256 + Synpcc7942_0671 0.20 +1.2 666,256 + Synpcc7942_0671 0.20 +0.9 666,256 + Synpcc7942_0671 0.20 -0.1 666,256 + Synpcc7942_0671 0.20 +0.3 666,264 - Synpcc7942_0671 0.22 -0.3 666,264 - Synpcc7942_0671 0.22 +2.2 666,324 - Synpcc7942_0671 0.34 +1.0 666,332 + Synpcc7942_0671 0.35 -0.1 666,340 - -0.1 666,348 - Synpcc7942_0671 0.39 +0.2 666,377 + Synpcc7942_0671 0.44 +1.2 666,377 + Synpcc7942_0671 0.44 +0.3 666,395 - Synpcc7942_0671 0.48 +1.5 666,396 - +1.5 666,407 + Synpcc7942_0671 0.50 -0.1 666,407 + Synpcc7942_0671 0.50 +0.3 666,407 + Synpcc7942_0671 0.50 +0.3 666,407 + Synpcc7942_0671 0.50 +0.5 666,414 + Synpcc7942_0671 0.52 -0.8 666,415 - Synpcc7942_0671 0.52 -0.4 666,415 - Synpcc7942_0671 0.52 -2.1 666,422 - Synpcc7942_0671 0.53 +1.1 666,426 + Synpcc7942_0671 0.54 +0.3 666,431 + Synpcc7942_0671 0.55 +1.1 666,476 + Synpcc7942_0671 0.64 -0.5 666,476 + Synpcc7942_0671 0.64 +0.0 666,476 + Synpcc7942_0671 0.64 +0.7 666,484 - Synpcc7942_0671 0.66 -1.1 666,484 - Synpcc7942_0671 0.66 +0.5 666,484 - Synpcc7942_0671 0.66 +1.2 666,489 - Synpcc7942_0671 0.67 +0.6 666,497 - Synpcc7942_0671 0.68 -0.1 666,499 + Synpcc7942_0671 0.69 +0.2 666,535 + Synpcc7942_0671 0.76 -0.3 666,535 + Synpcc7942_0671 0.76 -0.1 666,538 + Synpcc7942_0671 0.77 +0.1 666,545 + -0.1 666,633 - +0.3 666,635 + +0.3 666,638 - -0.7 666,656 - +0.3 666,676 + -0.6 666,676 - +0.2 666,683 + -1.1 666,684 - -1.9 666,691 - -0.4 666,752 - -1.1 666,763 - +0.9 666,784 - +2.5 666,872 + -0.6 666,910 + -0.1 666,920 + -1.8 666,924 + -1.4 666,924 + -0.8 666,924 + -0.8 666,956 + -0.4 666,964 - -1.1 666,971 + +0.7 666,993 + rpoD3 Synpcc7942_0672 0.11 -0.3 667,000 + rpoD3 Synpcc7942_0672 0.12 -1.6 667,038 + rpoD3 Synpcc7942_0672 0.16 -1.0 667,046 - rpoD3 Synpcc7942_0672 0.17 -0.3 667,046 - rpoD3 Synpcc7942_0672 0.17 -2.7 667,046 - rpoD3 Synpcc7942_0672 0.17 -1.1 667,072 + rpoD3 Synpcc7942_0672 0.19 -3.1 667,106 + rpoD3 Synpcc7942_0672 0.23 -0.1 667,115 + rpoD3 Synpcc7942_0672 0.24 -1.1 667,123 + rpoD3 Synpcc7942_0672 0.25 -0.4 667,178 - rpoD3 Synpcc7942_0672 0.30 -1.1 667,245 + rpoD3 Synpcc7942_0672 0.37 -0.9 667,253 - rpoD3 Synpcc7942_0672 0.38 +0.5 667,370 - -0.1 667,385 + rpoD3 Synpcc7942_0672 0.52 -1.1 667,479 + rpoD3 Synpcc7942_0672 0.61 -0.5 667,485 + rpoD3 Synpcc7942_0672 0.62 +0.4 667,485 + rpoD3 Synpcc7942_0672 0.62 -1.6 667,485 - rpoD3 Synpcc7942_0672 0.62 -3.4 667,488 + rpoD3 Synpcc7942_0672 0.62 -0.2 667,493 - rpoD3 Synpcc7942_0672 0.63 +1.1
Or see this region's nucleotide sequence