Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sqdB and sqdX are separated by 15 nucleotides sqdX and ftsI overlap by 46 nucleotides
Synpcc7942_0578: sqdB - UDP-sulfoquinovose synthase, at 558,408 to 559,616
sqdB
Synpcc7942_0579: sqdX - sulfolipid sulfoquinovosyldiacylglycerol biosynthesis protein, at 559,632 to 560,765
sqdX
Synpcc7942_0580: ftsI - Peptidoglycan glycosyltransferase, at 560,720 to 562,537
ftsI
Position (kb) 559
560
561 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 558.650 kb on + strand, within sqdB at 558.725 kb on + strand, within sqdB at 558.766 kb on - strand, within sqdB at 558.791 kb on - strand, within sqdB at 558.849 kb on - strand, within sqdB at 558.978 kb on - strand, within sqdB at 559.146 kb on + strand, within sqdB at 559.154 kb on - strand, within sqdB at 559.217 kb on - strand, within sqdB at 559.311 kb on - strand, within sqdB at 559.332 kb on - strand, within sqdB at 559.470 kb on + strand, within sqdB at 559.720 kb on + strand at 559.822 kb on - strand, within sqdX at 559.882 kb on - strand, within sqdX at 559.911 kb on + strand, within sqdX at 559.943 kb on + strand, within sqdX at 560.000 kb on + strand, within sqdX at 560.077 kb on + strand, within sqdX at 560.129 kb on + strand, within sqdX at 560.260 kb on - strand, within sqdX at 560.309 kb on + strand, within sqdX at 560.329 kb on + strand, within sqdX at 560.358 kb on - strand, within sqdX at 560.429 kb on - strand, within sqdX at 560.615 kb on + strand, within sqdX at 560.709 kb on + strand at 560.709 kb on + strand at 560.723 kb on - strand at 560.745 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 558,650 + sqdB Synpcc7942_0578 0.20 -1.8 558,725 + sqdB Synpcc7942_0578 0.26 -3.3 558,766 - sqdB Synpcc7942_0578 0.30 -1.5 558,791 - sqdB Synpcc7942_0578 0.32 -0.5 558,849 - sqdB Synpcc7942_0578 0.36 -2.3 558,978 - sqdB Synpcc7942_0578 0.47 -2.1 559,146 + sqdB Synpcc7942_0578 0.61 -2.1 559,154 - sqdB Synpcc7942_0578 0.62 -0.1 559,217 - sqdB Synpcc7942_0578 0.67 -0.5 559,311 - sqdB Synpcc7942_0578 0.75 -3.3 559,332 - sqdB Synpcc7942_0578 0.76 -2.7 559,470 + sqdB Synpcc7942_0578 0.88 -1.7 559,720 + -2.1 559,822 - sqdX Synpcc7942_0579 0.17 -3.4 559,882 - sqdX Synpcc7942_0579 0.22 -1.7 559,911 + sqdX Synpcc7942_0579 0.25 -2.9 559,943 + sqdX Synpcc7942_0579 0.27 -2.4 560,000 + sqdX Synpcc7942_0579 0.32 -2.1 560,077 + sqdX Synpcc7942_0579 0.39 +1.8 560,129 + sqdX Synpcc7942_0579 0.44 -1.4 560,260 - sqdX Synpcc7942_0579 0.55 -3.5 560,309 + sqdX Synpcc7942_0579 0.60 -1.1 560,329 + sqdX Synpcc7942_0579 0.61 -4.1 560,358 - sqdX Synpcc7942_0579 0.64 -1.7 560,429 - sqdX Synpcc7942_0579 0.70 -3.4 560,615 + sqdX Synpcc7942_0579 0.87 -0.1 560,709 + -1.1 560,709 + -2.3 560,723 - +0.1 560,745 + -2.4
Or see this region's nucleotide sequence