Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysA and Synpcc7942_0263 are separated by 79 nucleotides Synpcc7942_0263 and uppS overlap by 4 nucleotides uppS and Synpcc7942_0265 are separated by 12 nucleotides
Synpcc7942_0262: lysA - diaminopimelate decarboxylase, at 259,226 to 260,620
lysA
Synpcc7942_0263: Synpcc7942_0263 - Protein of unknown function DUF147, at 260,700 to 261,620
_0263
Synpcc7942_0264: uppS - Undecaprenyl pyrophosphate synthetase, at 261,617 to 262,372
uppS
Synpcc7942_0265: Synpcc7942_0265 - hypothetical protein, at 262,385 to 262,660
_0265
Position (kb) 260
261
262 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 260.601 kb on + strand at 260.601 kb on + strand at 260.655 kb on + strand at 260.663 kb on - strand at 260.663 kb on - strand at 260.667 kb on + strand at 260.667 kb on + strand at 260.667 kb on + strand at 260.667 kb on + strand at 260.675 kb on - strand at 260.675 kb on - strand at 260.675 kb on - strand at 260.675 kb on - strand at 260.675 kb on - strand at 260.690 kb on + strand at 260.690 kb on + strand at 260.690 kb on + strand at 260.696 kb on + strand at 260.698 kb on - strand at 260.704 kb on - strand at 260.834 kb on + strand, within Synpcc7942_0263 at 260.844 kb on + strand, within Synpcc7942_0263 at 260.849 kb on + strand, within Synpcc7942_0263 at 260.859 kb on + strand, within Synpcc7942_0263 at 260.904 kb on - strand, within Synpcc7942_0263 at 260.951 kb on + strand, within Synpcc7942_0263 at 260.951 kb on - strand, within Synpcc7942_0263 at 261.146 kb on - strand, within Synpcc7942_0263 at 261.195 kb on + strand, within Synpcc7942_0263 at 261.209 kb on + strand, within Synpcc7942_0263 at 261.289 kb on + strand, within Synpcc7942_0263 at 261.304 kb on + strand, within Synpcc7942_0263 at 262.367 kb on + strand at 262.381 kb on - strand at 262.383 kb on + strand at 262.386 kb on - strand at 262.397 kb on - strand at 262.417 kb on + strand, within Synpcc7942_0265 at 262.422 kb on + strand, within Synpcc7942_0265 at 262.422 kb on - strand, within Synpcc7942_0265 at 262.453 kb on + strand, within Synpcc7942_0265 at 262.454 kb on + strand, within Synpcc7942_0265 at 262.462 kb on - strand, within Synpcc7942_0265 at 262.479 kb on + strand, within Synpcc7942_0265 at 262.503 kb on + strand, within Synpcc7942_0265 at 262.503 kb on + strand, within Synpcc7942_0265 at 262.514 kb on + strand, within Synpcc7942_0265 at 262.535 kb on + strand, within Synpcc7942_0265 at 262.545 kb on + strand, within Synpcc7942_0265 at 262.553 kb on + strand, within Synpcc7942_0265 at 262.561 kb on - strand, within Synpcc7942_0265
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 260,601 + -1.1 260,601 + -0.6 260,655 + -1.7 260,663 - -2.1 260,663 - -0.0 260,667 + -3.3 260,667 + -0.7 260,667 + -0.5 260,667 + -1.4 260,675 - -1.4 260,675 - -0.7 260,675 - -0.1 260,675 - -1.0 260,675 - +0.5 260,690 + -1.2 260,690 + -1.1 260,690 + +1.7 260,696 + +0.2 260,698 - +0.7 260,704 - +0.7 260,834 + Synpcc7942_0263 0.15 -0.5 260,844 + Synpcc7942_0263 0.16 -2.9 260,849 + Synpcc7942_0263 0.16 -4.2 260,859 + Synpcc7942_0263 0.17 -1.1 260,904 - Synpcc7942_0263 0.22 +0.4 260,951 + Synpcc7942_0263 0.27 -2.7 260,951 - Synpcc7942_0263 0.27 -0.1 261,146 - Synpcc7942_0263 0.48 -2.1 261,195 + Synpcc7942_0263 0.54 -2.7 261,209 + Synpcc7942_0263 0.55 -1.1 261,289 + Synpcc7942_0263 0.64 -1.0 261,304 + Synpcc7942_0263 0.66 -2.9 262,367 + -0.1 262,381 - -1.7 262,383 + +0.1 262,386 - +0.4 262,397 - +0.7 262,417 + Synpcc7942_0265 0.12 -0.1 262,422 + Synpcc7942_0265 0.13 +0.1 262,422 - Synpcc7942_0265 0.13 -1.1 262,453 + Synpcc7942_0265 0.25 -1.4 262,454 + Synpcc7942_0265 0.25 +0.6 262,462 - Synpcc7942_0265 0.28 -1.1 262,479 + Synpcc7942_0265 0.34 +1.5 262,503 + Synpcc7942_0265 0.43 -0.4 262,503 + Synpcc7942_0265 0.43 -0.2 262,514 + Synpcc7942_0265 0.47 +0.2 262,535 + Synpcc7942_0265 0.54 -0.4 262,545 + Synpcc7942_0265 0.58 +1.2 262,553 + Synpcc7942_0265 0.61 -0.7 262,561 - Synpcc7942_0265 0.64 +2.1
Or see this region's nucleotide sequence