Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glk and Synpcc7942_0222 overlap by 4 nucleotides Synpcc7942_0222 and Synpcc7942_0223 overlap by 4 nucleotides Synpcc7942_0223 and psbN are separated by 15 nucleotides psbN and psbH are separated by 98 nucleotides psbH and Synpcc7942_0226 are separated by 93 nucleotides Synpcc7942_0226 and pth overlap by 4 nucleotides
Synpcc7942_0221: glk - glucokinase, at 221,498 to 222,535
glk
Synpcc7942_0222: Synpcc7942_0222 - hypothetical protein, at 222,532 to 223,398
_0222
Synpcc7942_0223: Synpcc7942_0223 - hypothetical protein, at 223,395 to 223,805
_0223
Synpcc7942_0224: psbN - photosystem II reaction center protein N, at 223,821 to 223,961
psbN
Synpcc7942_0225: psbH - photosystem II reaction center protein PsbH, at 224,060 to 224,263
psbH
Synpcc7942_0226: Synpcc7942_0226 - Sec-independent protein translocase TatA, at 224,357 to 224,632
_0226
Synpcc7942_0227: pth - peptidyl-tRNA hydrolase, at 224,629 to 225,255
pth
Position (kb)
223
224 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 222.413 kb on + strand, within glk at 222.427 kb on - strand, within glk at 222.430 kb on + strand, within glk at 222.438 kb on - strand at 222.438 kb on - strand at 222.438 kb on - strand at 222.446 kb on - strand at 222.446 kb on - strand at 222.490 kb on + strand at 222.503 kb on - strand at 222.503 kb on - strand at 222.530 kb on - strand at 222.742 kb on + strand, within Synpcc7942_0222 at 223.456 kb on + strand, within Synpcc7942_0223 at 223.482 kb on + strand, within Synpcc7942_0223 at 223.488 kb on - strand, within Synpcc7942_0223 at 223.535 kb on - strand, within Synpcc7942_0223 at 223.584 kb on - strand, within Synpcc7942_0223 at 223.607 kb on + strand, within Synpcc7942_0223 at 223.636 kb on + strand, within Synpcc7942_0223 at 223.644 kb on - strand, within Synpcc7942_0223 at 223.671 kb on + strand, within Synpcc7942_0223 at 223.677 kb on + strand, within Synpcc7942_0223 at 223.685 kb on - strand, within Synpcc7942_0223 at 223.685 kb on - strand, within Synpcc7942_0223 at 223.685 kb on - strand, within Synpcc7942_0223 at 223.765 kb on + strand at 223.773 kb on + strand at 223.781 kb on - strand at 223.781 kb on - strand at 223.783 kb on + strand at 223.807 kb on - strand at 223.818 kb on + strand at 223.818 kb on + strand at 223.818 kb on + strand at 223.818 kb on + strand at 223.818 kb on + strand at 223.818 kb on + strand at 223.818 kb on + strand at 223.824 kb on - strand at 223.824 kb on - strand at 223.826 kb on - strand at 223.826 kb on - strand at 223.826 kb on - strand at 223.826 kb on - strand at 223.854 kb on - strand, within psbN at 223.965 kb on + strand at 224.347 kb on + strand at 224.626 kb on + strand at 224.626 kb on + strand at 224.634 kb on - strand at 224.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 222,413 + glk Synpcc7942_0221 0.88 +1.1 222,427 - glk Synpcc7942_0221 0.89 +0.2 222,430 + glk Synpcc7942_0221 0.90 -2.1 222,438 - +1.5 222,438 - +0.3 222,438 - +1.4 222,446 - -0.5 222,446 - -1.1 222,490 + +0.1 222,503 - -0.9 222,503 - -1.9 222,530 - -0.9 222,742 + Synpcc7942_0222 0.24 -0.1 223,456 + Synpcc7942_0223 0.15 -0.1 223,482 + Synpcc7942_0223 0.21 +0.7 223,488 - Synpcc7942_0223 0.23 +1.3 223,535 - Synpcc7942_0223 0.34 -0.1 223,584 - Synpcc7942_0223 0.46 +1.5 223,607 + Synpcc7942_0223 0.52 +0.4 223,636 + Synpcc7942_0223 0.59 +1.5 223,644 - Synpcc7942_0223 0.61 +1.7 223,671 + Synpcc7942_0223 0.67 +1.1 223,677 + Synpcc7942_0223 0.69 +0.7 223,685 - Synpcc7942_0223 0.71 -1.3 223,685 - Synpcc7942_0223 0.71 -0.7 223,685 - Synpcc7942_0223 0.71 -0.8 223,765 + +1.5 223,773 + -1.1 223,781 - +2.0 223,781 - +0.6 223,783 + +1.5 223,807 - +0.9 223,818 + -0.3 223,818 + +1.1 223,818 + +0.5 223,818 + +1.6 223,818 + -0.6 223,818 + -1.1 223,818 + -2.4 223,824 - +1.1 223,824 - +0.1 223,826 - -1.7 223,826 - -1.0 223,826 - +1.0 223,826 - -1.1 223,854 - psbN Synpcc7942_0224 0.23 -2.1 223,965 + -0.7 224,347 + +1.7 224,626 + +0.7 224,626 + -2.3 224,634 - +1.5 224,634 - +0.5
Or see this region's nucleotide sequence