Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnN and Synpcc7942_0170 are separated by 150 nucleotides Synpcc7942_0170 and cysM are separated by 44 nucleotides cysM and Synpcc7942_0172 overlap by 4 nucleotides Synpcc7942_0172 and cysQ overlap by 4 nucleotides
Synpcc7942_0169: glnN - glutamate-ammonia ligase, glutamine synthetase type III, at 168,176 to 170,347
glnN
Synpcc7942_0170: Synpcc7942_0170 - hypothetical protein, at 170,498 to 170,752
_0170
Synpcc7942_0171: cysM - cysteine synthase A, at 170,797 to 171,915
cysM
Synpcc7942_0172: Synpcc7942_0172 - hypothetical protein, at 171,912 to 172,205
_0172
Synpcc7942_0173: cysQ - 3'-Phosphoadenosine 5'-phosphosulfate (PAPS) 3'-phosphatase-like, at 172,202 to 173,071
cysQ
Position (kb) 170
171
172 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 169.837 kb on + strand, within glnN at 169.845 kb on - strand, within glnN at 169.850 kb on - strand, within glnN at 169.860 kb on + strand, within glnN at 169.879 kb on + strand, within glnN at 169.887 kb on - strand, within glnN at 169.894 kb on - strand, within glnN at 169.904 kb on - strand, within glnN at 169.924 kb on + strand, within glnN at 169.965 kb on - strand, within glnN at 169.965 kb on - strand, within glnN at 169.977 kb on - strand, within glnN at 169.990 kb on + strand, within glnN at 170.053 kb on - strand at 170.073 kb on - strand, within glnN at 170.180 kb on + strand at 170.180 kb on + strand at 170.180 kb on + strand at 170.180 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.216 kb on + strand at 170.221 kb on + strand at 170.224 kb on - strand at 170.224 kb on - strand at 170.258 kb on + strand at 170.265 kb on - strand at 170.271 kb on - strand at 170.330 kb on + strand at 170.342 kb on + strand at 170.348 kb on + strand at 170.350 kb on - strand at 170.350 kb on - strand at 170.360 kb on + strand at 170.368 kb on - strand at 170.390 kb on + strand at 170.457 kb on - strand at 170.459 kb on - strand at 170.468 kb on - strand at 170.468 kb on - strand at 170.475 kb on - strand at 170.480 kb on - strand at 170.501 kb on - strand at 170.508 kb on + strand at 170.532 kb on + strand, within Synpcc7942_0170 at 170.566 kb on - strand, within Synpcc7942_0170 at 170.602 kb on - strand, within Synpcc7942_0170 at 170.646 kb on + strand, within Synpcc7942_0170 at 170.654 kb on - strand, within Synpcc7942_0170 at 170.656 kb on - strand, within Synpcc7942_0170 at 170.670 kb on - strand, within Synpcc7942_0170 at 170.672 kb on - strand, within Synpcc7942_0170 at 170.736 kb on + strand at 170.746 kb on + strand at 170.746 kb on + strand at 170.759 kb on + strand at 170.767 kb on - strand at 170.795 kb on - strand at 170.836 kb on + strand at 170.837 kb on + strand at 170.837 kb on + strand at 170.843 kb on + strand at 170.886 kb on - strand at 170.898 kb on + strand at 170.945 kb on + strand, within cysM at 170.988 kb on - strand, within cysM at 171.007 kb on + strand, within cysM at 171.161 kb on + strand, within cysM at 171.169 kb on - strand, within cysM at 171.174 kb on - strand, within cysM at 171.186 kb on + strand, within cysM at 171.189 kb on - strand, within cysM at 171.228 kb on + strand, within cysM at 171.244 kb on + strand, within cysM at 171.251 kb on + strand, within cysM at 171.274 kb on + strand, within cysM at 171.285 kb on + strand, within cysM at 171.293 kb on - strand, within cysM at 171.337 kb on + strand, within cysM at 171.361 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.559 kb on + strand at 171.559 kb on + strand, within cysM at 171.567 kb on - strand, within cysM at 171.567 kb on - strand, within cysM at 171.627 kb on - strand, within cysM at 171.687 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.695 kb on - strand, within cysM at 171.707 kb on + strand, within cysM at 171.713 kb on + strand, within cysM at 171.725 kb on + strand, within cysM at 171.746 kb on + strand, within cysM at 171.858 kb on + strand at 171.866 kb on - strand at 171.874 kb on - strand at 171.884 kb on - strand at 171.959 kb on + strand, within Synpcc7942_0172 at 171.959 kb on - strand, within Synpcc7942_0172 at 171.965 kb on - strand, within Synpcc7942_0172 at 171.969 kb on + strand, within Synpcc7942_0172 at 172.008 kb on - strand, within Synpcc7942_0172 at 172.061 kb on - strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.118 kb on - strand, within Synpcc7942_0172 at 172.204 kb on + strand at 172.212 kb on - strand at 172.222 kb on - strand at 172.239 kb on - strand at 172.243 kb on + strand at 172.296 kb on - strand, within cysQ at 172.310 kb on - strand, within cysQ at 172.323 kb on + strand, within cysQ at 172.323 kb on + strand, within cysQ at 172.323 kb on - strand, within cysQ at 172.334 kb on + strand, within cysQ at 172.360 kb on + strand, within cysQ at 172.360 kb on + strand, within cysQ at 172.368 kb on - strand, within cysQ at 172.406 kb on + strand, within cysQ at 172.426 kb on - strand, within cysQ at 172.438 kb on + strand, within cysQ at 172.472 kb on + strand, within cysQ at 172.477 kb on + strand, within cysQ at 172.477 kb on + strand, within cysQ at 172.485 kb on - strand, within cysQ at 172.612 kb on - strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.666 kb on + strand, within cysQ at 172.698 kb on + strand, within cysQ at 172.704 kb on - strand, within cysQ at 172.733 kb on + strand, within cysQ at 172.776 kb on + strand, within cysQ at 172.808 kb on - strand, within cysQ at 172.813 kb on - strand, within cysQ at 172.813 kb on - strand at 172.815 kb on + strand, within cysQ at 172.830 kb on + strand, within cysQ
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 169,837 + glnN Synpcc7942_0169 0.76 -1.5 169,845 - glnN Synpcc7942_0169 0.77 -1.0 169,850 - glnN Synpcc7942_0169 0.77 -0.5 169,860 + glnN Synpcc7942_0169 0.78 -0.1 169,879 + glnN Synpcc7942_0169 0.78 -2.4 169,887 - glnN Synpcc7942_0169 0.79 -1.1 169,894 - glnN Synpcc7942_0169 0.79 +0.7 169,904 - glnN Synpcc7942_0169 0.80 -1.7 169,924 + glnN Synpcc7942_0169 0.80 -0.4 169,965 - glnN Synpcc7942_0169 0.82 +0.3 169,965 - glnN Synpcc7942_0169 0.82 -0.4 169,977 - glnN Synpcc7942_0169 0.83 -1.3 169,990 + glnN Synpcc7942_0169 0.84 +1.0 170,053 - +0.9 170,073 - glnN Synpcc7942_0169 0.87 +0.0 170,180 + +2.5 170,180 + +0.2 170,180 + -0.9 170,180 + -0.7 170,216 + -0.8 170,216 + -0.3 170,216 + -0.2 170,216 + +0.8 170,216 + -1.7 170,221 + +0.4 170,224 - -1.0 170,224 - +1.9 170,258 + -1.1 170,265 - -1.0 170,271 - +0.8 170,330 + -0.6 170,342 + -0.8 170,348 + +0.3 170,350 - -0.2 170,350 - +0.9 170,360 + -0.1 170,368 - +0.5 170,390 + -0.3 170,457 - +0.7 170,459 - -2.1 170,468 - +2.1 170,468 - -1.7 170,475 - +0.3 170,480 - +0.3 170,501 - -0.1 170,508 + +0.4 170,532 + Synpcc7942_0170 0.13 +2.7 170,566 - Synpcc7942_0170 0.27 +0.2 170,602 - Synpcc7942_0170 0.41 -2.7 170,646 + Synpcc7942_0170 0.58 -2.1 170,654 - Synpcc7942_0170 0.61 -1.1 170,656 - Synpcc7942_0170 0.62 -1.7 170,670 - Synpcc7942_0170 0.67 +1.1 170,672 - Synpcc7942_0170 0.68 +0.7 170,736 + -0.3 170,746 + +0.5 170,746 + +0.7 170,759 + -1.1 170,767 - +0.2 170,795 - +3.1 170,836 + +0.9 170,837 + +0.7 170,837 + -2.9 170,843 + +0.8 170,886 - -1.4 170,898 + -2.7 170,945 + cysM Synpcc7942_0171 0.13 -2.4 170,988 - cysM Synpcc7942_0171 0.17 -2.4 171,007 + cysM Synpcc7942_0171 0.19 -2.9 171,161 + cysM Synpcc7942_0171 0.33 -1.1 171,169 - cysM Synpcc7942_0171 0.33 -1.7 171,174 - cysM Synpcc7942_0171 0.34 -1.7 171,186 + cysM Synpcc7942_0171 0.35 -2.1 171,189 - cysM Synpcc7942_0171 0.35 -2.1 171,228 + cysM Synpcc7942_0171 0.39 -3.6 171,244 + cysM Synpcc7942_0171 0.40 -2.4 171,251 + cysM Synpcc7942_0171 0.41 -2.4 171,274 + cysM Synpcc7942_0171 0.43 -2.9 171,285 + cysM Synpcc7942_0171 0.44 -1.7 171,293 - cysM Synpcc7942_0171 0.44 -0.1 171,337 + cysM Synpcc7942_0171 0.48 -3.4 171,361 + cysM Synpcc7942_0171 0.50 -2.6 171,515 + cysM Synpcc7942_0171 0.64 -1.1 171,515 + cysM Synpcc7942_0171 0.64 -2.4 171,515 + cysM Synpcc7942_0171 0.64 -2.4 171,515 + -0.1 171,523 - cysM Synpcc7942_0171 0.65 -0.1 171,523 - cysM Synpcc7942_0171 0.65 +0.6 171,523 - cysM Synpcc7942_0171 0.65 -2.1 171,523 - cysM Synpcc7942_0171 0.65 -1.1 171,559 + -0.1 171,559 + cysM Synpcc7942_0171 0.68 -0.7 171,567 - cysM Synpcc7942_0171 0.69 -2.7 171,567 - cysM Synpcc7942_0171 0.69 -2.9 171,627 - cysM Synpcc7942_0171 0.74 -2.4 171,687 + cysM Synpcc7942_0171 0.80 -2.4 171,694 + cysM Synpcc7942_0171 0.80 -2.1 171,694 + cysM Synpcc7942_0171 0.80 -3.1 171,695 - cysM Synpcc7942_0171 0.80 -2.4 171,707 + cysM Synpcc7942_0171 0.81 -1.1 171,713 + cysM Synpcc7942_0171 0.82 -3.0 171,725 + cysM Synpcc7942_0171 0.83 -1.9 171,746 + cysM Synpcc7942_0171 0.85 -1.4 171,858 + -0.9 171,866 - -0.4 171,874 - +0.9 171,884 - -1.7 171,959 + Synpcc7942_0172 0.16 +0.5 171,959 - Synpcc7942_0172 0.16 -1.1 171,965 - Synpcc7942_0172 0.18 +2.6 171,969 + Synpcc7942_0172 0.19 -2.6 172,008 - Synpcc7942_0172 0.33 -0.1 172,061 - Synpcc7942_0172 0.51 +0.6 172,101 + Synpcc7942_0172 0.64 +0.6 172,101 + Synpcc7942_0172 0.64 -1.0 172,101 + Synpcc7942_0172 0.64 -0.1 172,118 - Synpcc7942_0172 0.70 +0.5 172,204 + +0.4 172,212 - -0.4 172,222 - -1.1 172,239 - -2.1 172,243 + +0.7 172,296 - cysQ Synpcc7942_0173 0.11 -0.1 172,310 - cysQ Synpcc7942_0173 0.12 +0.6 172,323 + cysQ Synpcc7942_0173 0.14 +0.3 172,323 + cysQ Synpcc7942_0173 0.14 -0.2 172,323 - cysQ Synpcc7942_0173 0.14 +0.2 172,334 + cysQ Synpcc7942_0173 0.15 +0.9 172,360 + cysQ Synpcc7942_0173 0.18 -0.4 172,360 + cysQ Synpcc7942_0173 0.18 -0.1 172,368 - cysQ Synpcc7942_0173 0.19 +1.1 172,406 + cysQ Synpcc7942_0173 0.23 -0.7 172,426 - cysQ Synpcc7942_0173 0.26 -0.1 172,438 + cysQ Synpcc7942_0173 0.27 +3.5 172,472 + cysQ Synpcc7942_0173 0.31 +1.0 172,477 + cysQ Synpcc7942_0173 0.32 -0.7 172,477 + cysQ Synpcc7942_0173 0.32 +1.7 172,485 - cysQ Synpcc7942_0173 0.33 +0.9 172,612 - cysQ Synpcc7942_0173 0.47 +2.5 172,625 + cysQ Synpcc7942_0173 0.49 -1.5 172,625 + cysQ Synpcc7942_0173 0.49 -0.5 172,625 + cysQ Synpcc7942_0173 0.49 -1.1 172,625 + -0.5 172,631 + cysQ Synpcc7942_0173 0.49 -0.6 172,631 + cysQ Synpcc7942_0173 0.49 -0.7 172,631 + cysQ Synpcc7942_0173 0.49 -1.1 172,631 + cysQ Synpcc7942_0173 0.49 -1.1 172,631 + cysQ Synpcc7942_0173 0.49 +1.1 172,631 + cysQ Synpcc7942_0173 0.49 -2.1 172,631 + -0.1 172,631 + cysQ Synpcc7942_0173 0.49 -0.2 172,631 + cysQ Synpcc7942_0173 0.49 -2.9 172,639 - cysQ Synpcc7942_0173 0.50 +0.7 172,639 - cysQ Synpcc7942_0173 0.50 -0.1 172,639 - cysQ Synpcc7942_0173 0.50 -0.1 172,639 - cysQ Synpcc7942_0173 0.50 +0.0 172,639 - cysQ Synpcc7942_0173 0.50 -0.8 172,639 - cysQ Synpcc7942_0173 0.50 -0.1 172,639 - cysQ Synpcc7942_0173 0.50 +0.3 172,639 - cysQ Synpcc7942_0173 0.50 +0.0 172,639 - cysQ Synpcc7942_0173 0.50 +1.3 172,666 + cysQ Synpcc7942_0173 0.53 -0.1 172,698 + cysQ Synpcc7942_0173 0.57 +1.5 172,704 - cysQ Synpcc7942_0173 0.58 -0.1 172,733 + cysQ Synpcc7942_0173 0.61 -0.1 172,776 + cysQ Synpcc7942_0173 0.66 +0.1 172,808 - cysQ Synpcc7942_0173 0.70 +1.5 172,813 - cysQ Synpcc7942_0173 0.70 +0.2 172,813 - -0.1 172,815 + cysQ Synpcc7942_0173 0.70 -0.1 172,830 + cysQ Synpcc7942_0173 0.72 -1.9
Or see this region's nucleotide sequence