Experiment: D-Cycloserine 0.015625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0090 and ycf21 overlap by 4 nucleotides ycf21 and Synpcc7942_0092 are separated by 256 nucleotides Synpcc7942_0092 and tmk are separated by 98 nucleotides tmk and dnaX overlap by 1 nucleotides
Synpcc7942_0090: Synpcc7942_0090 - transcriptional regulator, GntR family, at 88,735 to 89,718
_0090
Synpcc7942_0091: ycf21 - conserved hypothetical protein YCF21, at 89,715 to 90,326
ycf21
Synpcc7942_0092: Synpcc7942_0092 - hypothetical protein, at 90,583 to 90,930
_0092
Synpcc7942_0093: tmk - Thymidylate kinase, at 91,029 to 91,676
tmk
Synpcc7942_0094: dnaX - DNA polymerase III subunit delta', at 91,676 to 92,611
dnaX
Position (kb)
90
91 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 89.739 kb on - strand at 89.749 kb on - strand at 90.365 kb on - strand at 90.433 kb on + strand at 90.436 kb on - strand at 90.464 kb on + strand at 90.464 kb on + strand at 90.469 kb on + strand at 90.469 kb on + strand at 90.472 kb on - strand at 90.472 kb on - strand at 90.472 kb on - strand at 90.472 kb on - strand at 90.472 kb on - strand at 90.477 kb on - strand at 90.485 kb on + strand at 90.503 kb on + strand at 90.503 kb on + strand at 90.503 kb on + strand at 90.511 kb on - strand at 90.511 kb on - strand at 90.511 kb on - strand at 90.539 kb on - strand at 90.547 kb on - strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.552 kb on + strand at 90.560 kb on - strand at 90.560 kb on - strand at 90.560 kb on - strand at 90.560 kb on - strand at 90.560 kb on - strand at 90.575 kb on - strand at 90.580 kb on + strand at 90.583 kb on - strand at 90.588 kb on - strand at 90.588 kb on - strand at 90.588 kb on - strand at 90.666 kb on + strand, within Synpcc7942_0092 at 90.674 kb on - strand, within Synpcc7942_0092 at 90.674 kb on - strand, within Synpcc7942_0092 at 90.676 kb on + strand, within Synpcc7942_0092 at 90.807 kb on + strand, within Synpcc7942_0092 at 90.807 kb on + strand, within Synpcc7942_0092 at 90.817 kb on - strand, within Synpcc7942_0092 at 90.844 kb on - strand, within Synpcc7942_0092 at 90.852 kb on + strand, within Synpcc7942_0092 at 90.860 kb on - strand, within Synpcc7942_0092 at 90.865 kb on + strand, within Synpcc7942_0092 at 90.865 kb on + strand, within Synpcc7942_0092 at 90.870 kb on + strand, within Synpcc7942_0092 at 90.870 kb on + strand, within Synpcc7942_0092 at 90.875 kb on + strand, within Synpcc7942_0092 at 90.878 kb on - strand, within Synpcc7942_0092 at 90.878 kb on - strand, within Synpcc7942_0092 at 90.883 kb on - strand, within Synpcc7942_0092 at 90.889 kb on - strand, within Synpcc7942_0092 at 90.958 kb on + strand at 91.007 kb on - strand at 91.022 kb on - strand at 91.027 kb on + strand at 91.029 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.015625 mg/ml remove 89,739 - -2.4 89,749 - +0.5 90,365 - +0.7 90,433 + -0.4 90,436 - -0.6 90,464 + +0.7 90,464 + +0.0 90,469 + +0.7 90,469 + +0.3 90,472 - +0.0 90,472 - +0.7 90,472 - +0.1 90,472 - -1.7 90,472 - -0.1 90,477 - +0.5 90,485 + +0.9 90,503 + +1.2 90,503 + +1.9 90,503 + -2.7 90,511 - +0.9 90,511 - +0.4 90,511 - +0.3 90,539 - -0.8 90,547 - +1.0 90,552 + +0.7 90,552 + -1.7 90,552 + +0.4 90,552 + +1.0 90,552 + -0.1 90,552 + +0.1 90,552 + +1.2 90,552 + +0.4 90,560 - +0.7 90,560 - +0.8 90,560 - -0.6 90,560 - -2.1 90,560 - -2.4 90,575 - -1.7 90,580 + -1.4 90,583 - +0.7 90,588 - -1.7 90,588 - -1.3 90,588 - -2.1 90,666 + Synpcc7942_0092 0.24 -2.1 90,674 - Synpcc7942_0092 0.26 -3.3 90,674 - Synpcc7942_0092 0.26 -1.5 90,676 + Synpcc7942_0092 0.27 -2.4 90,807 + Synpcc7942_0092 0.64 -0.8 90,807 + Synpcc7942_0092 0.64 -1.6 90,817 - Synpcc7942_0092 0.67 -3.0 90,844 - Synpcc7942_0092 0.75 -3.0 90,852 + Synpcc7942_0092 0.77 -0.8 90,860 - Synpcc7942_0092 0.80 -2.1 90,865 + Synpcc7942_0092 0.81 -1.8 90,865 + Synpcc7942_0092 0.81 -2.0 90,870 + Synpcc7942_0092 0.82 -0.1 90,870 + Synpcc7942_0092 0.82 -1.1 90,875 + Synpcc7942_0092 0.84 -2.7 90,878 - Synpcc7942_0092 0.85 +0.3 90,878 - Synpcc7942_0092 0.85 +1.5 90,883 - Synpcc7942_0092 0.86 -0.1 90,889 - Synpcc7942_0092 0.88 -1.3 90,958 + +1.2 91,007 - +0.4 91,022 - +0.1 91,027 + -1.7 91,029 - -0.3
Or see this region's nucleotide sequence