Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_866

Experiment: Putrescine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_865 and Pf1N1B4_866 are separated by 50 nucleotidesPf1N1B4_866 and Pf1N1B4_867 are separated by 295 nucleotides Pf1N1B4_865 - Cysteine desulfurase (EC 2.8.1.7), IscS subfamily, at 992,119 to 993,333 _865 Pf1N1B4_866 - Iron-sulfur cluster regulator IscR, at 993,384 to 993,845 _866 Pf1N1B4_867 - Serine acetyltransferase (EC 2.3.1.30), at 994,141 to 994,917 _867 Position (kb) 993 994Strain fitness (log2 ratio) -2 -1 0 1at 993.346 kb on - strandat 993.346 kb on - strandat 993.346 kb on - strandat 993.487 kb on - strand, within Pf1N1B4_866at 993.487 kb on - strand, within Pf1N1B4_866at 993.487 kb on - strand, within Pf1N1B4_866at 993.487 kb on - strand, within Pf1N1B4_866at 993.884 kb on - strandat 993.884 kb on - strandat 993.884 kb on - strandat 993.884 kb on - strandat 993.990 kb on - strandat 994.007 kb on + strandat 994.007 kb on + strandat 994.007 kb on + strandat 994.142 kb on + strandat 994.171 kb on + strandat 994.172 kb on - strandat 994.220 kb on + strand, within Pf1N1B4_867at 994.221 kb on - strand, within Pf1N1B4_867at 994.221 kb on - strand, within Pf1N1B4_867at 994.221 kb on - strand, within Pf1N1B4_867at 994.221 kb on - strand, within Pf1N1B4_867at 994.273 kb on + strand, within Pf1N1B4_867

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (C)
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993,346 - +0.0
993,346 - -1.0
993,346 - -1.0
993,487 - Pf1N1B4_866 0.22 -0.4
993,487 - Pf1N1B4_866 0.22 -1.8
993,487 - Pf1N1B4_866 0.22 -2.0
993,487 - Pf1N1B4_866 0.22 -0.5
993,884 - -0.5
993,884 - -1.8
993,884 - -2.3
993,884 - -1.8
993,990 - -1.0
994,007 + -0.5
994,007 + +0.0
994,007 + -0.4
994,142 + -2.0
994,171 + -1.8
994,172 - +0.9
994,220 + Pf1N1B4_867 0.10 +0.5
994,221 - Pf1N1B4_867 0.10 +0.8
994,221 - Pf1N1B4_867 0.10 +0.6
994,221 - Pf1N1B4_867 0.10 +0.5
994,221 - Pf1N1B4_867 0.10 -0.5
994,273 + Pf1N1B4_867 0.17 +0.6

Or see this region's nucleotide sequence