Experiment: Putrescine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_611 and Pf1N1B4_612 are separated by 133 nucleotides Pf1N1B4_612 and Pf1N1B4_613 overlap by 4 nucleotides Pf1N1B4_613 and Pf1N1B4_614 are separated by 446 nucleotides
Pf1N1B4_611 - CoA transferase, CAIB/BAIF family, at 705,554 to 706,885
_611
Pf1N1B4_612 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 707,019 to 707,882
_612
Pf1N1B4_613 - Arginine/ornithine antiporter ArcD, at 707,879 to 709,306
_613
Pf1N1B4_614 - Arginine/ornithine antiporter ArcD, at 709,753 to 711,180
_614
Position (kb)
707
708
709
710 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 706.997 kb on - strand at 707.063 kb on - strand at 707.209 kb on + strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.210 kb on - strand, within Pf1N1B4_612 at 707.397 kb on + strand, within Pf1N1B4_612 at 707.397 kb on + strand, within Pf1N1B4_612 at 707.398 kb on - strand at 707.398 kb on - strand, within Pf1N1B4_612 at 707.398 kb on - strand, within Pf1N1B4_612 at 707.398 kb on - strand, within Pf1N1B4_612 at 707.398 kb on - strand, within Pf1N1B4_612 at 707.398 kb on - strand, within Pf1N1B4_612 at 707.777 kb on - strand, within Pf1N1B4_612 at 707.851 kb on + strand at 707.852 kb on - strand at 707.901 kb on + strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.902 kb on - strand at 707.969 kb on - strand at 708.027 kb on + strand, within Pf1N1B4_613 at 708.028 kb on - strand, within Pf1N1B4_613 at 708.070 kb on - strand, within Pf1N1B4_613 at 708.070 kb on - strand, within Pf1N1B4_613 at 708.070 kb on - strand, within Pf1N1B4_613 at 708.070 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.148 kb on - strand, within Pf1N1B4_613 at 708.181 kb on - strand, within Pf1N1B4_613 at 708.199 kb on - strand, within Pf1N1B4_613 at 708.199 kb on - strand, within Pf1N1B4_613 at 708.199 kb on - strand, within Pf1N1B4_613 at 708.199 kb on - strand, within Pf1N1B4_613 at 708.228 kb on + strand, within Pf1N1B4_613 at 708.228 kb on + strand, within Pf1N1B4_613 at 708.229 kb on - strand, within Pf1N1B4_613 at 708.229 kb on - strand at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.243 kb on + strand, within Pf1N1B4_613 at 708.244 kb on - strand, within Pf1N1B4_613 at 708.244 kb on - strand, within Pf1N1B4_613 at 708.244 kb on - strand, within Pf1N1B4_613 at 708.244 kb on - strand, within Pf1N1B4_613 at 708.437 kb on - strand, within Pf1N1B4_613 at 708.481 kb on + strand, within Pf1N1B4_613 at 708.481 kb on + strand, within Pf1N1B4_613 at 708.481 kb on + strand, within Pf1N1B4_613 at 708.482 kb on - strand, within Pf1N1B4_613 at 708.592 kb on + strand, within Pf1N1B4_613 at 708.649 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.083 kb on + strand, within Pf1N1B4_613 at 709.083 kb on + strand, within Pf1N1B4_613 at 709.083 kb on + strand at 709.083 kb on + strand at 709.083 kb on + strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand at 709.084 kb on - strand, within Pf1N1B4_613 at 709.335 kb on - strand at 709.374 kb on - strand at 709.385 kb on - strand at 709.422 kb on - strand at 709.425 kb on + strand at 709.426 kb on - strand at 709.473 kb on + strand at 709.473 kb on + strand at 709.474 kb on - strand at 709.529 kb on + strand at 709.540 kb on + strand at 709.541 kb on - strand at 709.541 kb on - strand at 709.643 kb on + strand at 709.643 kb on + strand at 709.652 kb on - strand at 709.882 kb on - strand at 709.976 kb on - strand, within Pf1N1B4_614 at 710.005 kb on + strand, within Pf1N1B4_614 at 710.065 kb on + strand, within Pf1N1B4_614 at 710.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (C) remove 706,997 - -1.3 707,063 - -0.0 707,209 + Pf1N1B4_612 0.22 -2.8 707,210 - Pf1N1B4_612 0.22 +1.0 707,210 - Pf1N1B4_612 0.22 -1.0 707,210 - Pf1N1B4_612 0.22 +2.0 707,210 - Pf1N1B4_612 0.22 -1.0 707,210 - Pf1N1B4_612 0.22 -0.0 707,397 + Pf1N1B4_612 0.44 +0.7 707,397 + Pf1N1B4_612 0.44 -0.6 707,398 - +2.6 707,398 - Pf1N1B4_612 0.44 +1.0 707,398 - Pf1N1B4_612 0.44 -2.0 707,398 - Pf1N1B4_612 0.44 -0.0 707,398 - Pf1N1B4_612 0.44 -0.6 707,398 - Pf1N1B4_612 0.44 -0.4 707,777 - Pf1N1B4_612 0.88 -0.0 707,851 + +2.0 707,852 - -0.2 707,901 + +1.6 707,902 - +1.0 707,902 - +0.5 707,902 - +0.4 707,902 - +2.0 707,902 - +1.5 707,902 - +0.3 707,969 - -1.2 708,027 + Pf1N1B4_613 0.10 -0.4 708,028 - Pf1N1B4_613 0.10 +0.8 708,070 - Pf1N1B4_613 0.13 -1.0 708,070 - Pf1N1B4_613 0.13 -2.3 708,070 - Pf1N1B4_613 0.13 -0.0 708,070 - Pf1N1B4_613 0.13 +3.0 708,148 - Pf1N1B4_613 0.19 +1.6 708,148 - Pf1N1B4_613 0.19 -1.6 708,148 - Pf1N1B4_613 0.19 -0.0 708,148 - Pf1N1B4_613 0.19 -1.0 708,148 - Pf1N1B4_613 0.19 +0.4 708,181 - Pf1N1B4_613 0.21 +2.6 708,199 - Pf1N1B4_613 0.22 -0.5 708,199 - Pf1N1B4_613 0.22 -2.3 708,199 - Pf1N1B4_613 0.22 -0.3 708,199 - Pf1N1B4_613 0.22 -0.6 708,228 + Pf1N1B4_613 0.24 -0.6 708,228 + Pf1N1B4_613 0.24 +0.6 708,229 - Pf1N1B4_613 0.25 -0.0 708,229 - +1.6 708,243 + Pf1N1B4_613 0.25 -1.0 708,243 + Pf1N1B4_613 0.25 -2.0 708,243 + Pf1N1B4_613 0.25 -0.4 708,243 + Pf1N1B4_613 0.25 -0.4 708,243 + Pf1N1B4_613 0.25 -2.6 708,243 + Pf1N1B4_613 0.25 +1.8 708,243 + Pf1N1B4_613 0.25 +0.3 708,244 - Pf1N1B4_613 0.26 +0.7 708,244 - Pf1N1B4_613 0.26 -1.0 708,244 - Pf1N1B4_613 0.26 +0.3 708,244 - Pf1N1B4_613 0.26 +0.6 708,437 - Pf1N1B4_613 0.39 -0.0 708,481 + Pf1N1B4_613 0.42 +1.0 708,481 + Pf1N1B4_613 0.42 -1.0 708,481 + Pf1N1B4_613 0.42 -1.0 708,482 - Pf1N1B4_613 0.42 +1.4 708,592 + Pf1N1B4_613 0.50 -0.6 708,649 - Pf1N1B4_613 0.54 -1.0 709,054 - Pf1N1B4_613 0.82 +1.6 709,054 - Pf1N1B4_613 0.82 +1.6 709,054 - Pf1N1B4_613 0.82 -0.0 709,054 - Pf1N1B4_613 0.82 -0.0 709,083 + Pf1N1B4_613 0.84 +0.7 709,083 + Pf1N1B4_613 0.84 -0.0 709,083 + +1.6 709,083 + -0.0 709,083 + Pf1N1B4_613 0.84 -1.0 709,084 - Pf1N1B4_613 0.84 -0.6 709,084 - Pf1N1B4_613 0.84 +0.5 709,084 - Pf1N1B4_613 0.84 -0.0 709,084 - Pf1N1B4_613 0.84 -1.0 709,084 - Pf1N1B4_613 0.84 +0.3 709,084 - +2.2 709,084 - Pf1N1B4_613 0.84 +0.4 709,335 - +0.3 709,374 - -2.6 709,385 - -0.0 709,422 - -1.6 709,425 + -0.0 709,426 - +0.2 709,473 + -0.0 709,473 + -0.4 709,474 - -1.8 709,529 + -2.0 709,540 + +0.7 709,541 - -1.0 709,541 - -0.3 709,643 + -1.6 709,643 + +0.0 709,652 - +0.4 709,882 - +1.3 709,976 - Pf1N1B4_614 0.16 +1.0 710,005 + Pf1N1B4_614 0.18 -2.3 710,065 + Pf1N1B4_614 0.22 -0.3 710,065 + +0.0
Or see this region's nucleotide sequence