Experiment: Putrescine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4499 and Pf1N1B4_4500 are separated by 147 nucleotides Pf1N1B4_4500 and Pf1N1B4_4501 overlap by 24 nucleotides Pf1N1B4_4501 and Pf1N1B4_4502 are separated by 240 nucleotides
Pf1N1B4_4499 - SoxH protein, homolog, at 4,960,425 to 4,961,333
_4499
Pf1N1B4_4500 - hypothetical protein, at 4,961,481 to 4,961,648
_4500
Pf1N1B4_4501 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 4,961,625 to 4,963,400
_4501
Pf1N1B4_4502 - Aldehyde dehydrogenase (EC 1.2.1.3), at 4,963,641 to 4,965,161
_4502
Position (kb)
4961
4962
4963
4964 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4960.828 kb on - strand, within Pf1N1B4_4499 at 4961.509 kb on - strand at 4961.516 kb on + strand, within Pf1N1B4_4500 at 4961.598 kb on - strand, within Pf1N1B4_4500 at 4961.916 kb on + strand, within Pf1N1B4_4501 at 4961.916 kb on + strand, within Pf1N1B4_4501 at 4961.917 kb on - strand, within Pf1N1B4_4501 at 4961.917 kb on - strand, within Pf1N1B4_4501 at 4961.917 kb on - strand, within Pf1N1B4_4501 at 4962.156 kb on + strand, within Pf1N1B4_4501 at 4962.315 kb on + strand, within Pf1N1B4_4501 at 4962.315 kb on + strand, within Pf1N1B4_4501 at 4962.315 kb on + strand, within Pf1N1B4_4501 at 4962.315 kb on + strand, within Pf1N1B4_4501 at 4962.316 kb on - strand, within Pf1N1B4_4501 at 4962.510 kb on + strand at 4962.510 kb on + strand, within Pf1N1B4_4501 at 4962.627 kb on + strand, within Pf1N1B4_4501 at 4962.627 kb on + strand at 4962.628 kb on - strand, within Pf1N1B4_4501 at 4962.628 kb on - strand, within Pf1N1B4_4501 at 4962.687 kb on + strand, within Pf1N1B4_4501 at 4962.787 kb on - strand at 4962.787 kb on - strand, within Pf1N1B4_4501 at 4962.862 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.915 kb on + strand, within Pf1N1B4_4501 at 4962.916 kb on - strand at 4962.916 kb on - strand, within Pf1N1B4_4501 at 4962.919 kb on + strand, within Pf1N1B4_4501 at 4962.919 kb on + strand, within Pf1N1B4_4501 at 4962.966 kb on + strand, within Pf1N1B4_4501 at 4963.026 kb on + strand, within Pf1N1B4_4501 at 4963.027 kb on - strand, within Pf1N1B4_4501 at 4963.263 kb on + strand at 4963.264 kb on - strand at 4963.523 kb on + strand at 4963.523 kb on + strand at 4963.524 kb on - strand at 4963.584 kb on + strand at 4963.584 kb on + strand at 4963.584 kb on + strand at 4963.585 kb on - strand at 4963.647 kb on + strand at 4963.648 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (C) remove 4,960,828 - Pf1N1B4_4499 0.44 -2.1 4,961,509 - +1.1 4,961,516 + Pf1N1B4_4500 0.21 -1.2 4,961,598 - Pf1N1B4_4500 0.70 -1.3 4,961,916 + Pf1N1B4_4501 0.16 -0.2 4,961,916 + Pf1N1B4_4501 0.16 -0.0 4,961,917 - Pf1N1B4_4501 0.16 -0.9 4,961,917 - Pf1N1B4_4501 0.16 +0.5 4,961,917 - Pf1N1B4_4501 0.16 +1.3 4,962,156 + Pf1N1B4_4501 0.30 -1.1 4,962,315 + Pf1N1B4_4501 0.39 +1.1 4,962,315 + Pf1N1B4_4501 0.39 -0.5 4,962,315 + Pf1N1B4_4501 0.39 +2.1 4,962,315 + Pf1N1B4_4501 0.39 -0.1 4,962,316 - Pf1N1B4_4501 0.39 +0.7 4,962,510 + +1.5 4,962,510 + Pf1N1B4_4501 0.50 +0.5 4,962,627 + Pf1N1B4_4501 0.56 -1.7 4,962,627 + -1.5 4,962,628 - Pf1N1B4_4501 0.56 -1.2 4,962,628 - Pf1N1B4_4501 0.56 +0.5 4,962,687 + Pf1N1B4_4501 0.60 +0.4 4,962,787 - +0.1 4,962,787 - Pf1N1B4_4501 0.65 +2.3 4,962,862 + Pf1N1B4_4501 0.70 +2.3 4,962,915 + -0.5 4,962,915 + Pf1N1B4_4501 0.73 -0.3 4,962,915 + Pf1N1B4_4501 0.73 -0.5 4,962,915 + Pf1N1B4_4501 0.73 +0.5 4,962,915 + Pf1N1B4_4501 0.73 +1.3 4,962,915 + Pf1N1B4_4501 0.73 +0.5 4,962,916 - -0.5 4,962,916 - Pf1N1B4_4501 0.73 +0.1 4,962,919 + Pf1N1B4_4501 0.73 +0.5 4,962,919 + Pf1N1B4_4501 0.73 -0.1 4,962,966 + Pf1N1B4_4501 0.76 +1.5 4,963,026 + Pf1N1B4_4501 0.79 +0.3 4,963,027 - Pf1N1B4_4501 0.79 +0.8 4,963,263 + +1.5 4,963,264 - -1.2 4,963,523 + +0.5 4,963,523 + +1.3 4,963,524 - -0.4 4,963,584 + -0.5 4,963,584 + -1.8 4,963,584 + +0.5 4,963,585 - -1.5 4,963,647 + +0.7 4,963,648 - -0.8
Or see this region's nucleotide sequence