Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2919

Experiment: Putrescine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2918 and Pf1N1B4_2919 overlap by 4 nucleotidesPf1N1B4_2919 and Pf1N1B4_2920 are separated by 0 nucleotidesPf1N1B4_2920 and Pf1N1B4_2921 overlap by 7 nucleotides Pf1N1B4_2918 - Glutamyl-tRNA reductase (EC 1.2.1.70), at 3,276,265 to 3,277,554 _2918 Pf1N1B4_2919 - Peptide chain release factor 1, at 3,277,551 to 3,278,633 _2919 Pf1N1B4_2920 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 3,278,634 to 3,279,464 _2920 Pf1N1B4_2921 - Molybdopterin biosynthesis protein MoeB, at 3,279,458 to 3,280,213 _2921 Position (kb) 3277 3278 3279Strain fitness (log2 ratio) -2 -1 0 1at 3277.527 kb on + strandat 3278.623 kb on + strandat 3278.631 kb on + strandat 3278.631 kb on + strandat 3279.477 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (C)
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3,277,527 + -0.2
3,278,623 + -0.4
3,278,631 + -1.9
3,278,631 + +1.1
3,279,477 + +0.5

Or see this region's nucleotide sequence