Experiment: Putrescine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2253 and Pf1N1B4_2254 are separated by 227 nucleotides Pf1N1B4_2254 and Pf1N1B4_2255 are separated by 374 nucleotides
Pf1N1B4_2253 - Transcriptional regulator, TetR family, at 2,568,719 to 2,569,351
_2253
Pf1N1B4_2254 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data) , at 2,569,579 to 2,570,859
_2254
Pf1N1B4_2255 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94), at 2,571,234 to 2,572,010
_2255
Position (kb)
2569
2570
2571 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2568.741 kb on + strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.765 kb on + strand at 2568.765 kb on + strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.036 kb on - strand, within Pf1N1B4_2253 at 2569.233 kb on + strand, within Pf1N1B4_2253 at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.429 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.494 kb on - strand at 2569.524 kb on + strand at 2569.524 kb on + strand at 2569.545 kb on + strand at 2569.580 kb on + strand at 2569.595 kb on + strand at 2569.596 kb on - strand at 2569.604 kb on + strand at 2569.604 kb on + strand at 2569.604 kb on + strand at 2569.604 kb on + strand at 2569.605 kb on - strand at 2569.605 kb on - strand at 2569.605 kb on - strand at 2569.605 kb on - strand at 2569.654 kb on - strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.677 kb on - strand at 2569.677 kb on - strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.772 kb on + strand, within Pf1N1B4_2254 at 2569.772 kb on + strand, within Pf1N1B4_2254 at 2569.773 kb on - strand, within Pf1N1B4_2254 at 2569.773 kb on - strand, within Pf1N1B4_2254 at 2569.786 kb on + strand, within Pf1N1B4_2254 at 2569.786 kb on + strand, within Pf1N1B4_2254 at 2569.787 kb on - strand, within Pf1N1B4_2254 at 2569.942 kb on - strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2570.003 kb on + strand, within Pf1N1B4_2254 at 2570.130 kb on + strand, within Pf1N1B4_2254 at 2570.220 kb on - strand, within Pf1N1B4_2254 at 2570.397 kb on - strand, within Pf1N1B4_2254 at 2570.397 kb on - strand, within Pf1N1B4_2254 at 2570.397 kb on - strand, within Pf1N1B4_2254 at 2570.420 kb on + strand, within Pf1N1B4_2254 at 2570.421 kb on - strand, within Pf1N1B4_2254 at 2570.507 kb on + strand, within Pf1N1B4_2254 at 2570.508 kb on - strand, within Pf1N1B4_2254 at 2570.508 kb on - strand, within Pf1N1B4_2254 at 2570.632 kb on + strand, within Pf1N1B4_2254 at 2570.633 kb on - strand, within Pf1N1B4_2254 at 2570.761 kb on - strand at 2570.773 kb on + strand at 2570.773 kb on + strand at 2570.773 kb on + strand at 2570.774 kb on - strand at 2570.774 kb on - strand at 2570.774 kb on - strand at 2570.805 kb on - strand at 2570.805 kb on - strand at 2570.859 kb on + strand at 2570.882 kb on + strand at 2570.883 kb on - strand at 2570.883 kb on - strand at 2570.982 kb on - strand at 2571.201 kb on - strand at 2571.217 kb on + strand at 2571.217 kb on + strand at 2571.218 kb on - strand at 2571.218 kb on - strand at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.319 kb on + strand, within Pf1N1B4_2255 at 2571.320 kb on - strand, within Pf1N1B4_2255 at 2571.379 kb on + strand, within Pf1N1B4_2255 at 2571.380 kb on - strand, within Pf1N1B4_2255 at 2571.380 kb on - strand, within Pf1N1B4_2255 at 2571.380 kb on - strand, within Pf1N1B4_2255 at 2571.380 kb on - strand, within Pf1N1B4_2255 at 2571.440 kb on + strand, within Pf1N1B4_2255 at 2571.440 kb on + strand, within Pf1N1B4_2255 at 2571.440 kb on + strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.443 kb on + strand at 2571.443 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.459 kb on + strand, within Pf1N1B4_2255 at 2571.459 kb on + strand, within Pf1N1B4_2255 at 2571.461 kb on + strand, within Pf1N1B4_2255 at 2571.485 kb on + strand, within Pf1N1B4_2255 at 2571.486 kb on - strand, within Pf1N1B4_2255 at 2571.486 kb on - strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.728 kb on - strand, within Pf1N1B4_2255
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (C) remove 2,568,741 + +0.1 2,568,742 - +0.4 2,568,742 - -0.6 2,568,742 - +1.0 2,568,754 - -0.2 2,568,754 - -0.6 2,568,754 - +0.1 2,568,765 + -0.6 2,568,765 + -0.2 2,568,766 - -0.1 2,568,766 - +0.4 2,568,766 - +2.7 2,568,840 + Pf1N1B4_2253 0.19 -1.2 2,568,840 + Pf1N1B4_2253 0.19 +0.9 2,568,840 + Pf1N1B4_2253 0.19 +2.7 2,568,840 + Pf1N1B4_2253 0.19 +1.7 2,568,840 + Pf1N1B4_2253 0.19 -0.5 2,568,840 + Pf1N1B4_2253 0.19 +0.4 2,568,840 + +0.4 2,568,840 + Pf1N1B4_2253 0.19 +1.0 2,568,840 + Pf1N1B4_2253 0.19 +1.0 2,568,840 + Pf1N1B4_2253 0.19 +1.0 2,568,840 + Pf1N1B4_2253 0.19 +1.0 2,568,840 + Pf1N1B4_2253 0.19 -0.2 2,568,840 + Pf1N1B4_2253 0.19 -1.2 2,568,840 + Pf1N1B4_2253 0.19 +1.1 2,568,841 - Pf1N1B4_2253 0.19 +1.0 2,568,841 - Pf1N1B4_2253 0.19 +0.4 2,568,841 - Pf1N1B4_2253 0.19 +0.4 2,568,841 - Pf1N1B4_2253 0.19 -0.6 2,568,841 - Pf1N1B4_2253 0.19 +0.4 2,568,841 - +0.4 2,568,841 - Pf1N1B4_2253 0.19 -0.8 2,568,841 - Pf1N1B4_2253 0.19 +1.0 2,568,841 - Pf1N1B4_2253 0.19 +1.2 2,569,035 + Pf1N1B4_2253 0.50 -0.6 2,569,035 + Pf1N1B4_2253 0.50 -0.4 2,569,035 + Pf1N1B4_2253 0.50 -0.4 2,569,036 - Pf1N1B4_2253 0.50 -0.8 2,569,233 + Pf1N1B4_2253 0.81 +0.4 2,569,381 + +0.9 2,569,381 + -0.0 2,569,381 + -1.5 2,569,382 - -1.1 2,569,382 - -1.1 2,569,382 - +0.4 2,569,429 + -0.2 2,569,464 + -0.2 2,569,464 + +0.4 2,569,464 + -1.6 2,569,464 + +1.0 2,569,464 + -0.9 2,569,464 + -0.6 2,569,464 + -0.4 2,569,464 + -2.2 2,569,464 + -0.6 2,569,464 + +0.6 2,569,464 + +2.0 2,569,464 + -0.0 2,569,464 + -1.2 2,569,465 - -1.2 2,569,465 - -1.6 2,569,465 - -0.2 2,569,465 - +1.4 2,569,465 - -0.4 2,569,465 - -0.9 2,569,465 - +0.4 2,569,465 - -0.6 2,569,465 - -0.4 2,569,465 - +1.4 2,569,465 - +1.2 2,569,465 - -1.2 2,569,465 - +0.9 2,569,465 - -0.9 2,569,465 - +1.0 2,569,494 - +1.8 2,569,524 + +1.6 2,569,524 + +1.0 2,569,545 + -1.4 2,569,580 + -2.3 2,569,595 + -2.4 2,569,596 - -1.9 2,569,604 + -1.2 2,569,604 + -1.8 2,569,604 + +0.4 2,569,604 + -0.6 2,569,605 - -3.5 2,569,605 - -1.2 2,569,605 - -1.2 2,569,605 - -3.1 2,569,654 - -1.4 2,569,676 + -1.2 2,569,676 + +0.4 2,569,676 + -0.9 2,569,676 + -0.6 2,569,676 + -1.4 2,569,677 - +0.4 2,569,677 - -1.6 2,569,691 + -2.2 2,569,691 + -2.4 2,569,691 + -1.6 2,569,691 + -3.1 2,569,691 + -2.7 2,569,692 - -0.9 2,569,692 - -1.2 2,569,692 - -0.6 2,569,692 - -0.0 2,569,692 - -1.6 2,569,692 - -1.2 2,569,772 + Pf1N1B4_2254 0.15 -0.6 2,569,772 + Pf1N1B4_2254 0.15 -0.2 2,569,773 - Pf1N1B4_2254 0.15 +0.7 2,569,773 - Pf1N1B4_2254 0.15 -1.8 2,569,786 + Pf1N1B4_2254 0.16 -2.4 2,569,786 + Pf1N1B4_2254 0.16 -1.6 2,569,787 - Pf1N1B4_2254 0.16 -2.4 2,569,942 - Pf1N1B4_2254 0.28 -2.4 2,569,979 + Pf1N1B4_2254 0.31 +0.4 2,569,979 + Pf1N1B4_2254 0.31 -2.1 2,569,979 + Pf1N1B4_2254 0.31 -0.6 2,569,979 + Pf1N1B4_2254 0.31 -3.6 2,569,979 + Pf1N1B4_2254 0.31 -3.4 2,569,979 + Pf1N1B4_2254 0.31 -0.2 2,569,979 + Pf1N1B4_2254 0.31 -0.9 2,569,979 + Pf1N1B4_2254 0.31 -1.6 2,569,979 + Pf1N1B4_2254 0.31 -1.2 2,569,979 + Pf1N1B4_2254 0.31 -0.6 2,569,980 - Pf1N1B4_2254 0.31 -0.0 2,569,980 - Pf1N1B4_2254 0.31 -0.6 2,569,980 - Pf1N1B4_2254 0.31 +1.4 2,569,980 - Pf1N1B4_2254 0.31 -2.2 2,569,980 - Pf1N1B4_2254 0.31 -2.3 2,569,980 - Pf1N1B4_2254 0.31 -0.6 2,570,003 + Pf1N1B4_2254 0.33 -0.2 2,570,130 + Pf1N1B4_2254 0.43 -2.2 2,570,220 - Pf1N1B4_2254 0.50 -0.6 2,570,397 - Pf1N1B4_2254 0.64 -2.4 2,570,397 - Pf1N1B4_2254 0.64 -1.9 2,570,397 - Pf1N1B4_2254 0.64 -2.4 2,570,420 + Pf1N1B4_2254 0.66 -0.6 2,570,421 - Pf1N1B4_2254 0.66 -2.3 2,570,507 + Pf1N1B4_2254 0.72 +0.4 2,570,508 - Pf1N1B4_2254 0.73 -2.1 2,570,508 - Pf1N1B4_2254 0.73 -1.6 2,570,632 + Pf1N1B4_2254 0.82 -3.2 2,570,633 - Pf1N1B4_2254 0.82 -2.2 2,570,761 - -1.2 2,570,773 + +0.4 2,570,773 + -2.2 2,570,773 + -0.6 2,570,774 - +0.4 2,570,774 - -2.2 2,570,774 - -2.1 2,570,805 - -1.6 2,570,805 - -2.3 2,570,859 + -0.6 2,570,882 + -0.6 2,570,883 - -1.6 2,570,883 - -0.0 2,570,982 - -1.4 2,571,201 - +1.1 2,571,217 + +0.4 2,571,217 + -0.8 2,571,218 - -1.9 2,571,218 - -1.4 2,571,315 + Pf1N1B4_2255 0.10 -1.9 2,571,315 + Pf1N1B4_2255 0.10 -0.6 2,571,315 + Pf1N1B4_2255 0.10 +0.4 2,571,315 + Pf1N1B4_2255 0.10 -0.0 2,571,315 + Pf1N1B4_2255 0.10 +0.6 2,571,315 + +0.4 2,571,315 + Pf1N1B4_2255 0.10 +0.2 2,571,315 + Pf1N1B4_2255 0.10 -0.1 2,571,315 + Pf1N1B4_2255 0.10 +1.1 2,571,316 - Pf1N1B4_2255 0.11 -0.4 2,571,316 - Pf1N1B4_2255 0.11 -0.0 2,571,316 - Pf1N1B4_2255 0.11 -0.8 2,571,316 - Pf1N1B4_2255 0.11 -0.2 2,571,316 - Pf1N1B4_2255 0.11 -0.4 2,571,316 - Pf1N1B4_2255 0.11 +0.4 2,571,319 + Pf1N1B4_2255 0.11 -0.6 2,571,320 - Pf1N1B4_2255 0.11 -1.6 2,571,379 + Pf1N1B4_2255 0.19 +0.4 2,571,380 - Pf1N1B4_2255 0.19 -0.9 2,571,380 - Pf1N1B4_2255 0.19 +0.1 2,571,380 - Pf1N1B4_2255 0.19 -1.4 2,571,380 - Pf1N1B4_2255 0.19 -0.6 2,571,440 + Pf1N1B4_2255 0.27 +0.4 2,571,440 + Pf1N1B4_2255 0.27 +0.4 2,571,440 + Pf1N1B4_2255 0.27 -0.6 2,571,441 - Pf1N1B4_2255 0.27 +1.4 2,571,441 - Pf1N1B4_2255 0.27 -0.8 2,571,441 - Pf1N1B4_2255 0.27 -0.0 2,571,441 - Pf1N1B4_2255 0.27 +1.2 2,571,441 - Pf1N1B4_2255 0.27 -0.8 2,571,443 + +1.4 2,571,443 + Pf1N1B4_2255 0.27 -0.1 2,571,445 + Pf1N1B4_2255 0.27 -0.4 2,571,445 + Pf1N1B4_2255 0.27 -0.9 2,571,445 + Pf1N1B4_2255 0.27 -0.5 2,571,445 + Pf1N1B4_2255 0.27 -0.6 2,571,445 + Pf1N1B4_2255 0.27 +0.4 2,571,445 + Pf1N1B4_2255 0.27 +0.4 2,571,445 + Pf1N1B4_2255 0.27 -0.4 2,571,445 + Pf1N1B4_2255 0.27 +0.8 2,571,446 - Pf1N1B4_2255 0.27 +0.4 2,571,446 - Pf1N1B4_2255 0.27 +1.4 2,571,446 - Pf1N1B4_2255 0.27 -0.6 2,571,446 - Pf1N1B4_2255 0.27 +1.4 2,571,446 - Pf1N1B4_2255 0.27 -1.0 2,571,446 - Pf1N1B4_2255 0.27 -1.2 2,571,446 - +0.4 2,571,446 - Pf1N1B4_2255 0.27 +0.4 2,571,446 - Pf1N1B4_2255 0.27 -0.5 2,571,446 - Pf1N1B4_2255 0.27 -1.2 2,571,459 + Pf1N1B4_2255 0.29 +1.4 2,571,459 + Pf1N1B4_2255 0.29 -0.3 2,571,461 + Pf1N1B4_2255 0.29 +0.4 2,571,485 + Pf1N1B4_2255 0.32 +2.4 2,571,486 - Pf1N1B4_2255 0.32 -0.6 2,571,486 - Pf1N1B4_2255 0.32 -1.8 2,571,727 + Pf1N1B4_2255 0.63 +0.6 2,571,727 + Pf1N1B4_2255 0.63 -0.6 2,571,727 + Pf1N1B4_2255 0.63 -0.2 2,571,727 + Pf1N1B4_2255 0.63 +2.0 2,571,727 + Pf1N1B4_2255 0.63 +0.4 2,571,728 - Pf1N1B4_2255 0.64 -0.6
Or see this region's nucleotide sequence