Experiment: Putrescine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2142 and Pf1N1B4_2143 are separated by 116 nucleotides Pf1N1B4_2143 and Pf1N1B4_2144 are separated by 156 nucleotides Pf1N1B4_2144 and Pf1N1B4_2145 overlap by 29 nucleotides Pf1N1B4_2145 and Pf1N1B4_2146 are separated by 97 nucleotides
Pf1N1B4_2142 - FIG00954130: hypothetical protein, at 2,453,424 to 2,453,777
_2142
Pf1N1B4_2143 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 2,453,894 to 2,454,382
_2143
Pf1N1B4_2144 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 2,454,539 to 2,455,843
_2144
Pf1N1B4_2145 - Translation initiation inhibitor, at 2,455,815 to 2,456,168
_2145
Pf1N1B4_2146 - Alanine racemase (EC 5.1.1.1), at 2,456,266 to 2,457,339
_2146
Position (kb)
2454
2455
2456 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.628 kb on + strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.629 kb on - strand, within Pf1N1B4_2142 at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.745 kb on + strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.746 kb on - strand at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.944 kb on + strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.945 kb on - strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.946 kb on + strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2453.947 kb on - strand, within Pf1N1B4_2143 at 2454.077 kb on + strand, within Pf1N1B4_2143 at 2454.077 kb on + strand, within Pf1N1B4_2143 at 2454.077 kb on + strand, within Pf1N1B4_2143 at 2454.077 kb on + strand, within Pf1N1B4_2143 at 2454.078 kb on - strand, within Pf1N1B4_2143 at 2454.078 kb on - strand, within Pf1N1B4_2143 at 2454.078 kb on - strand, within Pf1N1B4_2143 at 2454.122 kb on - strand, within Pf1N1B4_2143 at 2454.122 kb on - strand, within Pf1N1B4_2143 at 2454.122 kb on - strand, within Pf1N1B4_2143 at 2454.136 kb on - strand, within Pf1N1B4_2143 at 2454.169 kb on + strand, within Pf1N1B4_2143 at 2454.169 kb on + strand, within Pf1N1B4_2143 at 2454.169 kb on + strand, within Pf1N1B4_2143 at 2454.169 kb on + strand, within Pf1N1B4_2143 at 2454.170 kb on - strand, within Pf1N1B4_2143 at 2454.170 kb on - strand, within Pf1N1B4_2143 at 2454.170 kb on - strand, within Pf1N1B4_2143 at 2454.170 kb on - strand, within Pf1N1B4_2143 at 2454.205 kb on - strand, within Pf1N1B4_2143 at 2454.383 kb on - strand at 2454.443 kb on - strand at 2454.443 kb on - strand at 2454.444 kb on + strand at 2454.445 kb on - strand at 2454.473 kb on + strand at 2454.502 kb on + strand at 2454.503 kb on - strand at 2454.503 kb on - strand at 2454.533 kb on - strand at 2454.587 kb on + strand at 2454.587 kb on + strand at 2454.587 kb on + strand at 2454.587 kb on + strand at 2454.588 kb on - strand at 2454.588 kb on - strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.590 kb on + strand at 2454.689 kb on + strand, within Pf1N1B4_2144 at 2454.689 kb on + strand, within Pf1N1B4_2144 at 2454.794 kb on + strand, within Pf1N1B4_2144 at 2454.794 kb on + strand, within Pf1N1B4_2144 at 2454.794 kb on + strand, within Pf1N1B4_2144 at 2454.878 kb on + strand, within Pf1N1B4_2144 at 2454.927 kb on - strand, within Pf1N1B4_2144 at 2455.283 kb on + strand, within Pf1N1B4_2144 at 2455.283 kb on + strand, within Pf1N1B4_2144 at 2455.305 kb on - strand, within Pf1N1B4_2144 at 2455.305 kb on - strand, within Pf1N1B4_2144 at 2455.349 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.430 kb on + strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.431 kb on - strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.432 kb on + strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.433 kb on - strand, within Pf1N1B4_2144 at 2455.774 kb on + strand at 2455.774 kb on + strand at 2455.874 kb on + strand, within Pf1N1B4_2145 at 2456.013 kb on + strand, within Pf1N1B4_2145 at 2456.251 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (C) remove 2,453,628 + Pf1N1B4_2142 0.58 -0.5 2,453,628 + Pf1N1B4_2142 0.58 -1.9 2,453,628 + Pf1N1B4_2142 0.58 +0.5 2,453,628 + Pf1N1B4_2142 0.58 -0.1 2,453,628 + Pf1N1B4_2142 0.58 -0.2 2,453,628 + Pf1N1B4_2142 0.58 +0.2 2,453,628 + Pf1N1B4_2142 0.58 -1.1 2,453,628 + Pf1N1B4_2142 0.58 -2.1 2,453,628 + Pf1N1B4_2142 0.58 -1.5 2,453,628 + Pf1N1B4_2142 0.58 +0.0 2,453,629 - Pf1N1B4_2142 0.58 +0.0 2,453,629 - Pf1N1B4_2142 0.58 +0.4 2,453,629 - Pf1N1B4_2142 0.58 +1.5 2,453,629 - Pf1N1B4_2142 0.58 -0.8 2,453,629 - Pf1N1B4_2142 0.58 -0.5 2,453,629 - Pf1N1B4_2142 0.58 +0.0 2,453,629 - Pf1N1B4_2142 0.58 +0.1 2,453,745 + -0.4 2,453,745 + -0.5 2,453,745 + +2.0 2,453,745 + +0.9 2,453,745 + +0.5 2,453,745 + +2.5 2,453,745 + -0.1 2,453,745 + -1.2 2,453,745 + -0.1 2,453,745 + -0.0 2,453,745 + -0.2 2,453,745 + -1.3 2,453,745 + +1.0 2,453,745 + +1.5 2,453,745 + +0.9 2,453,745 + +1.0 2,453,745 + +0.0 2,453,745 + -1.5 2,453,745 + +0.9 2,453,745 + -0.5 2,453,746 - +1.2 2,453,746 - +0.5 2,453,746 - -0.5 2,453,746 - +0.5 2,453,746 - -1.5 2,453,746 - +0.5 2,453,746 - +0.5 2,453,746 - -1.9 2,453,746 - -0.2 2,453,746 - +1.5 2,453,746 - +0.0 2,453,746 - -0.5 2,453,746 - +2.5 2,453,746 - +0.0 2,453,944 + Pf1N1B4_2143 0.10 -0.7 2,453,944 + Pf1N1B4_2143 0.10 -1.5 2,453,944 + Pf1N1B4_2143 0.10 -1.1 2,453,944 + Pf1N1B4_2143 0.10 -0.5 2,453,944 + Pf1N1B4_2143 0.10 -0.5 2,453,944 + Pf1N1B4_2143 0.10 -2.7 2,453,945 - Pf1N1B4_2143 0.10 -1.1 2,453,945 - Pf1N1B4_2143 0.10 -1.4 2,453,945 - Pf1N1B4_2143 0.10 -1.7 2,453,945 - Pf1N1B4_2143 0.10 -1.5 2,453,945 - Pf1N1B4_2143 0.10 -1.4 2,453,945 - Pf1N1B4_2143 0.10 -2.1 2,453,946 + Pf1N1B4_2143 0.11 -1.1 2,453,946 + Pf1N1B4_2143 0.11 -1.1 2,453,946 + Pf1N1B4_2143 0.11 -0.5 2,453,946 + Pf1N1B4_2143 0.11 -1.9 2,453,946 + Pf1N1B4_2143 0.11 -0.7 2,453,946 + Pf1N1B4_2143 0.11 +1.7 2,453,946 + Pf1N1B4_2143 0.11 -1.9 2,453,947 - Pf1N1B4_2143 0.11 -1.1 2,453,947 - Pf1N1B4_2143 0.11 +0.2 2,453,947 - Pf1N1B4_2143 0.11 -0.1 2,453,947 - Pf1N1B4_2143 0.11 -2.1 2,453,947 - Pf1N1B4_2143 0.11 -0.1 2,453,947 - Pf1N1B4_2143 0.11 -2.0 2,453,947 - Pf1N1B4_2143 0.11 -1.2 2,453,947 - Pf1N1B4_2143 0.11 -1.5 2,453,947 - Pf1N1B4_2143 0.11 -1.1 2,453,947 - Pf1N1B4_2143 0.11 -0.9 2,453,947 - Pf1N1B4_2143 0.11 -0.5 2,453,947 - Pf1N1B4_2143 0.11 -1.3 2,453,947 - Pf1N1B4_2143 0.11 -2.0 2,453,947 - Pf1N1B4_2143 0.11 -1.5 2,453,947 - Pf1N1B4_2143 0.11 -0.9 2,453,947 - Pf1N1B4_2143 0.11 -0.5 2,454,077 + Pf1N1B4_2143 0.37 -2.5 2,454,077 + Pf1N1B4_2143 0.37 +0.5 2,454,077 + Pf1N1B4_2143 0.37 -1.9 2,454,077 + Pf1N1B4_2143 0.37 +1.3 2,454,078 - Pf1N1B4_2143 0.38 -1.5 2,454,078 - Pf1N1B4_2143 0.38 -2.3 2,454,078 - Pf1N1B4_2143 0.38 -1.5 2,454,122 - Pf1N1B4_2143 0.47 -1.3 2,454,122 - Pf1N1B4_2143 0.47 -0.3 2,454,122 - Pf1N1B4_2143 0.47 -0.5 2,454,136 - Pf1N1B4_2143 0.49 -2.1 2,454,169 + Pf1N1B4_2143 0.56 -0.1 2,454,169 + Pf1N1B4_2143 0.56 -1.1 2,454,169 + Pf1N1B4_2143 0.56 -1.2 2,454,169 + Pf1N1B4_2143 0.56 -0.5 2,454,170 - Pf1N1B4_2143 0.56 -1.1 2,454,170 - Pf1N1B4_2143 0.56 -1.1 2,454,170 - Pf1N1B4_2143 0.56 -2.2 2,454,170 - Pf1N1B4_2143 0.56 -0.5 2,454,205 - Pf1N1B4_2143 0.64 -0.5 2,454,383 - +0.1 2,454,443 - -1.1 2,454,443 - -1.9 2,454,444 + -0.4 2,454,445 - -1.6 2,454,473 + +0.1 2,454,502 + +1.0 2,454,503 - -0.9 2,454,503 - -1.1 2,454,533 - -1.1 2,454,587 + -0.7 2,454,587 + -1.1 2,454,587 + -1.1 2,454,587 + -1.5 2,454,588 - -1.9 2,454,588 - -3.2 2,454,590 + +0.8 2,454,590 + -2.2 2,454,590 + -0.5 2,454,590 + -0.3 2,454,590 + -1.9 2,454,590 + -1.5 2,454,590 + +0.5 2,454,590 + -1.5 2,454,590 + -1.1 2,454,590 + -0.8 2,454,689 + Pf1N1B4_2144 0.11 +0.9 2,454,689 + Pf1N1B4_2144 0.11 -0.1 2,454,794 + Pf1N1B4_2144 0.20 -0.6 2,454,794 + Pf1N1B4_2144 0.20 -1.1 2,454,794 + Pf1N1B4_2144 0.20 -1.5 2,454,878 + Pf1N1B4_2144 0.26 -1.9 2,454,927 - Pf1N1B4_2144 0.30 -1.7 2,455,283 + Pf1N1B4_2144 0.57 -1.1 2,455,283 + Pf1N1B4_2144 0.57 -3.4 2,455,305 - Pf1N1B4_2144 0.59 -1.1 2,455,305 - Pf1N1B4_2144 0.59 -1.9 2,455,349 + Pf1N1B4_2144 0.62 -1.5 2,455,430 + Pf1N1B4_2144 0.68 -0.5 2,455,430 + Pf1N1B4_2144 0.68 +1.5 2,455,430 + Pf1N1B4_2144 0.68 -1.9 2,455,430 + Pf1N1B4_2144 0.68 +0.0 2,455,430 + Pf1N1B4_2144 0.68 -0.5 2,455,430 + Pf1N1B4_2144 0.68 -1.0 2,455,430 + Pf1N1B4_2144 0.68 -0.1 2,455,430 + Pf1N1B4_2144 0.68 +0.5 2,455,430 + Pf1N1B4_2144 0.68 -1.1 2,455,430 + Pf1N1B4_2144 0.68 -0.8 2,455,430 + Pf1N1B4_2144 0.68 -1.0 2,455,430 + Pf1N1B4_2144 0.68 +0.0 2,455,430 + Pf1N1B4_2144 0.68 -1.1 2,455,430 + Pf1N1B4_2144 0.68 -0.9 2,455,430 + Pf1N1B4_2144 0.68 +0.5 2,455,430 + Pf1N1B4_2144 0.68 -1.5 2,455,430 + Pf1N1B4_2144 0.68 +0.5 2,455,430 + Pf1N1B4_2144 0.68 -0.9 2,455,430 + Pf1N1B4_2144 0.68 -0.5 2,455,430 + Pf1N1B4_2144 0.68 +0.0 2,455,430 + Pf1N1B4_2144 0.68 -0.5 2,455,430 + Pf1N1B4_2144 0.68 -0.8 2,455,430 + Pf1N1B4_2144 0.68 -1.5 2,455,430 + Pf1N1B4_2144 0.68 -0.2 2,455,431 - Pf1N1B4_2144 0.68 -1.5 2,455,431 - Pf1N1B4_2144 0.68 -1.2 2,455,431 - Pf1N1B4_2144 0.68 -1.9 2,455,431 - Pf1N1B4_2144 0.68 -0.7 2,455,431 - Pf1N1B4_2144 0.68 -1.1 2,455,431 - Pf1N1B4_2144 0.68 -1.1 2,455,432 + Pf1N1B4_2144 0.68 -1.5 2,455,432 + Pf1N1B4_2144 0.68 +0.5 2,455,432 + Pf1N1B4_2144 0.68 -0.3 2,455,432 + Pf1N1B4_2144 0.68 -1.9 2,455,432 + Pf1N1B4_2144 0.68 -2.4 2,455,433 - Pf1N1B4_2144 0.69 -1.9 2,455,433 - Pf1N1B4_2144 0.69 -2.7 2,455,433 - Pf1N1B4_2144 0.69 -0.1 2,455,774 + -0.8 2,455,774 + -0.5 2,455,874 + Pf1N1B4_2145 0.17 -0.1 2,456,013 + Pf1N1B4_2145 0.56 -0.3 2,456,251 + -0.5
Or see this region's nucleotide sequence