Experiment: Putrescine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1733 and Pf1N1B4_1734 are separated by 180 nucleotides Pf1N1B4_1734 and Pf1N1B4_5186 are separated by 347 nucleotides Pf1N1B4_5186 and Pf1N1B4_1735 are separated by 468 nucleotides
Pf1N1B4_1733 - 5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 1,981,191 to 1,982,468
_1733
Pf1N1B4_1734 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 1,982,649 to 1,984,091
_1734
Pf1N1B4_5186 - tRNA-Arg-CCG, at 1,984,439 to 1,984,512
_5186
Pf1N1B4_1735 - hypothetical protein, at 1,984,981 to 1,985,133
_1735
Position (kb)
1982
1983
1984
1985 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1981.743 kb on + strand, within Pf1N1B4_1733 at 1981.743 kb on + strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.761 kb on + strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.823 kb on - strand, within Pf1N1B4_1733 at 1981.823 kb on - strand, within Pf1N1B4_1733 at 1982.015 kb on + strand, within Pf1N1B4_1733 at 1982.053 kb on + strand, within Pf1N1B4_1733 at 1982.053 kb on + strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.218 kb on + strand, within Pf1N1B4_1733 at 1982.219 kb on - strand, within Pf1N1B4_1733 at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.531 kb on + strand at 1982.532 kb on - strand at 1982.532 kb on - strand at 1982.532 kb on - strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.651 kb on - strand at 1982.651 kb on - strand at 1982.651 kb on - strand at 1982.668 kb on + strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.683 kb on + strand at 1982.683 kb on + strand at 1982.698 kb on + strand at 1982.699 kb on - strand at 1982.699 kb on - strand at 1982.795 kb on - strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1983.187 kb on + strand, within Pf1N1B4_1734 at 1983.205 kb on + strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1984.083 kb on - strand at 1984.083 kb on - strand at 1984.083 kb on - strand at 1984.132 kb on + strand at 1984.132 kb on + strand at 1984.132 kb on + strand at 1984.132 kb on + strand at 1984.132 kb on + strand at 1984.133 kb on - strand at 1984.140 kb on + strand at 1984.140 kb on + strand at 1984.140 kb on + strand at 1984.140 kb on + strand at 1984.140 kb on + strand at 1984.141 kb on - strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.155 kb on + strand at 1984.156 kb on - strand at 1984.156 kb on - strand at 1984.156 kb on - strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.196 kb on + strand at 1984.201 kb on - strand at 1984.267 kb on + strand at 1984.267 kb on + strand at 1984.267 kb on + strand at 1984.267 kb on + strand at 1984.268 kb on - strand at 1984.282 kb on + strand at 1984.283 kb on - strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.516 kb on + strand at 1984.517 kb on - strand at 1984.517 kb on - strand at 1984.517 kb on - strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.520 kb on + strand at 1984.521 kb on - strand at 1984.569 kb on - strand at 1984.592 kb on + strand at 1984.593 kb on - strand at 1984.593 kb on - strand at 1984.593 kb on - strand at 1984.695 kb on - strand at 1984.707 kb on - strand at 1984.743 kb on - strand at 1984.743 kb on - strand at 1984.762 kb on - strand at 1984.790 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (C) remove 1,981,743 + Pf1N1B4_1733 0.43 +0.4 1,981,743 + Pf1N1B4_1733 0.43 -0.9 1,981,744 - Pf1N1B4_1733 0.43 -2.6 1,981,744 - Pf1N1B4_1733 0.43 -0.6 1,981,744 - Pf1N1B4_1733 0.43 -1.9 1,981,744 - Pf1N1B4_1733 0.43 -1.2 1,981,761 + Pf1N1B4_1733 0.45 -1.2 1,981,762 - Pf1N1B4_1733 0.45 -1.4 1,981,762 - Pf1N1B4_1733 0.45 -1.9 1,981,762 - Pf1N1B4_1733 0.45 -1.2 1,981,762 - Pf1N1B4_1733 0.45 -1.3 1,981,762 - Pf1N1B4_1733 0.45 -0.2 1,981,762 - Pf1N1B4_1733 0.45 -1.2 1,981,762 - Pf1N1B4_1733 0.45 -2.6 1,981,823 - Pf1N1B4_1733 0.49 -2.2 1,981,823 - Pf1N1B4_1733 0.49 -1.4 1,982,015 + Pf1N1B4_1733 0.64 -2.2 1,982,053 + Pf1N1B4_1733 0.67 -3.0 1,982,053 + Pf1N1B4_1733 0.67 -1.5 1,982,210 - Pf1N1B4_1733 0.80 -0.3 1,982,210 - Pf1N1B4_1733 0.80 +0.4 1,982,210 - Pf1N1B4_1733 0.80 -0.6 1,982,210 - Pf1N1B4_1733 0.80 -1.9 1,982,218 + Pf1N1B4_1733 0.80 -1.6 1,982,219 - Pf1N1B4_1733 0.80 -0.3 1,982,468 + +0.4 1,982,468 + -1.2 1,982,468 + -1.9 1,982,468 + +0.4 1,982,468 + -2.2 1,982,468 + -2.2 1,982,469 - -0.6 1,982,469 - -1.2 1,982,469 - -1.8 1,982,469 - +0.4 1,982,469 - +0.4 1,982,469 - +1.4 1,982,531 + -2.6 1,982,532 - +0.7 1,982,532 - +0.4 1,982,532 - -0.6 1,982,650 + -1.2 1,982,650 + +0.4 1,982,650 + -2.2 1,982,650 + +0.4 1,982,650 + -2.0 1,982,650 + -0.2 1,982,650 + -1.2 1,982,651 - -1.2 1,982,651 - +0.4 1,982,651 - -2.2 1,982,668 + -0.6 1,982,669 - -1.2 1,982,669 - -0.6 1,982,669 - -0.2 1,982,669 - -2.4 1,982,683 + -0.6 1,982,683 + -3.3 1,982,698 + -3.0 1,982,699 - -2.2 1,982,699 - +0.4 1,982,795 - Pf1N1B4_1734 0.10 -2.6 1,982,842 + Pf1N1B4_1734 0.13 -0.6 1,982,842 + Pf1N1B4_1734 0.13 -2.2 1,982,842 + Pf1N1B4_1734 0.13 -0.3 1,982,842 + Pf1N1B4_1734 0.13 -0.6 1,982,842 + Pf1N1B4_1734 0.13 -1.2 1,982,843 - Pf1N1B4_1734 0.13 -1.6 1,982,843 - Pf1N1B4_1734 0.13 -1.2 1,982,843 - Pf1N1B4_1734 0.13 +0.4 1,982,843 - Pf1N1B4_1734 0.13 -1.9 1,982,843 - Pf1N1B4_1734 0.13 -2.7 1,982,843 - Pf1N1B4_1734 0.13 -0.6 1,982,843 - Pf1N1B4_1734 0.13 -1.6 1,982,843 - Pf1N1B4_1734 0.13 -0.6 1,982,843 - Pf1N1B4_1734 0.13 -1.2 1,982,843 - Pf1N1B4_1734 0.13 +1.9 1,982,843 - Pf1N1B4_1734 0.13 -1.6 1,982,843 - Pf1N1B4_1734 0.13 -1.6 1,982,848 + Pf1N1B4_1734 0.14 -3.3 1,982,848 + Pf1N1B4_1734 0.14 -0.6 1,982,848 + Pf1N1B4_1734 0.14 -1.9 1,982,848 + Pf1N1B4_1734 0.14 -1.2 1,982,848 + Pf1N1B4_1734 0.14 -1.6 1,982,848 + Pf1N1B4_1734 0.14 +0.4 1,982,849 - Pf1N1B4_1734 0.14 -1.2 1,982,849 - Pf1N1B4_1734 0.14 -0.6 1,982,849 - Pf1N1B4_1734 0.14 -1.6 1,983,187 + Pf1N1B4_1734 0.37 -1.6 1,983,205 + Pf1N1B4_1734 0.39 -1.6 1,983,206 - Pf1N1B4_1734 0.39 -2.2 1,983,206 - Pf1N1B4_1734 0.39 -1.2 1,983,206 - Pf1N1B4_1734 0.39 -1.9 1,984,083 - -2.6 1,984,083 - -0.6 1,984,083 - -3.8 1,984,132 + -0.6 1,984,132 + +0.4 1,984,132 + -0.6 1,984,132 + -1.5 1,984,132 + +0.4 1,984,133 - -1.9 1,984,140 + -1.6 1,984,140 + -2.6 1,984,140 + -2.9 1,984,140 + -0.6 1,984,140 + -1.2 1,984,141 - +0.4 1,984,155 + -0.6 1,984,155 + -1.6 1,984,155 + -1.6 1,984,155 + -2.2 1,984,155 + +1.1 1,984,155 + -1.2 1,984,155 + -0.6 1,984,156 - -1.2 1,984,156 - +0.4 1,984,156 - +0.4 1,984,196 + -0.6 1,984,196 + -1.6 1,984,196 + -1.6 1,984,196 + -0.6 1,984,196 + -0.3 1,984,196 + -1.6 1,984,196 + -0.7 1,984,196 + -0.6 1,984,201 - -0.2 1,984,267 + -1.2 1,984,267 + +0.4 1,984,267 + -1.9 1,984,267 + -0.6 1,984,268 - -0.6 1,984,282 + -1.2 1,984,283 - -1.9 1,984,516 + +2.7 1,984,516 + +0.3 1,984,516 + -1.2 1,984,516 + -1.2 1,984,516 + +1.4 1,984,516 + +0.4 1,984,516 + +1.4 1,984,516 + -0.1 1,984,516 + +0.1 1,984,516 + +0.4 1,984,516 + -1.4 1,984,516 + -0.2 1,984,516 + +0.2 1,984,516 + -1.2 1,984,517 - -0.2 1,984,517 - -0.6 1,984,517 - -0.6 1,984,520 + -1.6 1,984,520 + +0.1 1,984,520 + +0.2 1,984,520 + +0.4 1,984,520 + +1.2 1,984,520 + +1.4 1,984,520 + +0.0 1,984,520 + +0.4 1,984,520 + +1.4 1,984,520 + +0.4 1,984,521 - +1.0 1,984,569 - -1.3 1,984,592 + -2.6 1,984,593 - -0.9 1,984,593 - +0.6 1,984,593 - -0.5 1,984,695 - +0.4 1,984,707 - -0.2 1,984,743 - +0.7 1,984,743 - +0.4 1,984,762 - +1.4 1,984,790 - +0.4
Or see this region's nucleotide sequence