Experiment: Putrescine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1219 and Pf1N1B4_1220 are separated by 220 nucleotides Pf1N1B4_1220 and Pf1N1B4_1221 are separated by 156 nucleotides
Pf1N1B4_1219 - Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2), at 1,382,749 to 1,383,906
_1219
Pf1N1B4_1220 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 1,384,127 to 1,385,002
_1220
Pf1N1B4_1221 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase, at 1,385,159 to 1,386,304
_1221
Position (kb)
1384
1385
1386 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1383.200 kb on + strand, within Pf1N1B4_1219 at 1383.203 kb on + strand, within Pf1N1B4_1219 at 1383.216 kb on - strand, within Pf1N1B4_1219 at 1383.229 kb on - strand, within Pf1N1B4_1219 at 1383.440 kb on - strand, within Pf1N1B4_1219 at 1383.456 kb on - strand, within Pf1N1B4_1219 at 1383.456 kb on - strand, within Pf1N1B4_1219 at 1383.497 kb on + strand, within Pf1N1B4_1219 at 1383.569 kb on + strand, within Pf1N1B4_1219 at 1383.569 kb on + strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.599 kb on + strand, within Pf1N1B4_1219 at 1383.600 kb on - strand, within Pf1N1B4_1219 at 1383.772 kb on + strand, within Pf1N1B4_1219 at 1383.772 kb on + strand, within Pf1N1B4_1219 at 1383.772 kb on + strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.806 kb on + strand at 1383.806 kb on + strand at 1383.895 kb on - strand at 1383.916 kb on + strand at 1383.916 kb on + strand at 1383.917 kb on - strand at 1383.923 kb on + strand at 1383.923 kb on + strand at 1383.924 kb on - strand at 1383.924 kb on - strand at 1383.927 kb on + strand at 1383.967 kb on - strand at 1384.023 kb on - strand at 1384.097 kb on - strand at 1384.128 kb on + strand at 1384.129 kb on - strand at 1384.129 kb on - strand at 1384.129 kb on - strand at 1384.285 kb on + strand, within Pf1N1B4_1220 at 1384.426 kb on - strand, within Pf1N1B4_1220 at 1384.426 kb on - strand, within Pf1N1B4_1220 at 1384.426 kb on - strand, within Pf1N1B4_1220 at 1384.665 kb on + strand, within Pf1N1B4_1220 at 1384.666 kb on - strand, within Pf1N1B4_1220 at 1384.666 kb on - strand, within Pf1N1B4_1220 at 1384.666 kb on - strand, within Pf1N1B4_1220 at 1384.732 kb on + strand, within Pf1N1B4_1220 at 1384.732 kb on + strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand at 1384.987 kb on + strand at 1384.988 kb on - strand at 1384.988 kb on - strand at 1385.069 kb on - strand at 1385.102 kb on + strand at 1385.103 kb on - strand at 1385.103 kb on - strand at 1385.106 kb on + strand at 1385.136 kb on + strand at 1385.136 kb on + strand at 1385.136 kb on + strand at 1385.137 kb on - strand at 1385.137 kb on - strand at 1385.137 kb on - strand at 1385.137 kb on - strand at 1385.174 kb on + strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.354 kb on + strand, within Pf1N1B4_1221 at 1385.354 kb on + strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.441 kb on + strand, within Pf1N1B4_1221 at 1385.501 kb on - strand, within Pf1N1B4_1221 at 1385.533 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.733 kb on - strand, within Pf1N1B4_1221 at 1385.762 kb on - strand, within Pf1N1B4_1221 at 1385.872 kb on + strand, within Pf1N1B4_1221 at 1385.872 kb on + strand, within Pf1N1B4_1221 at 1385.873 kb on - strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (C) remove 1,383,200 + Pf1N1B4_1219 0.39 +0.8 1,383,203 + Pf1N1B4_1219 0.39 -0.8 1,383,216 - Pf1N1B4_1219 0.40 +1.2 1,383,229 - Pf1N1B4_1219 0.41 -0.8 1,383,440 - Pf1N1B4_1219 0.60 +0.2 1,383,456 - Pf1N1B4_1219 0.61 +1.0 1,383,456 - Pf1N1B4_1219 0.61 +2.0 1,383,497 + Pf1N1B4_1219 0.65 -2.2 1,383,569 + Pf1N1B4_1219 0.71 -0.0 1,383,569 + Pf1N1B4_1219 0.71 -0.4 1,383,570 - Pf1N1B4_1219 0.71 -0.2 1,383,570 - Pf1N1B4_1219 0.71 -1.4 1,383,570 - Pf1N1B4_1219 0.71 +3.2 1,383,570 - Pf1N1B4_1219 0.71 -1.0 1,383,570 - Pf1N1B4_1219 0.71 -0.8 1,383,570 - Pf1N1B4_1219 0.71 +1.2 1,383,570 - Pf1N1B4_1219 0.71 +0.2 1,383,570 - Pf1N1B4_1219 0.71 +1.3 1,383,570 - Pf1N1B4_1219 0.71 -0.6 1,383,570 - Pf1N1B4_1219 0.71 +0.8 1,383,570 - Pf1N1B4_1219 0.71 +0.8 1,383,570 - Pf1N1B4_1219 0.71 +0.2 1,383,599 + Pf1N1B4_1219 0.73 +0.7 1,383,600 - Pf1N1B4_1219 0.73 -0.6 1,383,772 + Pf1N1B4_1219 0.88 +0.2 1,383,772 + Pf1N1B4_1219 0.88 +0.8 1,383,772 + Pf1N1B4_1219 0.88 -0.2 1,383,773 - Pf1N1B4_1219 0.88 +0.4 1,383,773 - Pf1N1B4_1219 0.88 -0.6 1,383,773 - Pf1N1B4_1219 0.88 -0.2 1,383,773 - Pf1N1B4_1219 0.88 +0.4 1,383,773 - Pf1N1B4_1219 0.88 -0.1 1,383,773 - Pf1N1B4_1219 0.88 +0.3 1,383,773 - Pf1N1B4_1219 0.88 -1.1 1,383,773 - Pf1N1B4_1219 0.88 -0.3 1,383,773 - Pf1N1B4_1219 0.88 +1.2 1,383,773 - Pf1N1B4_1219 0.88 +0.7 1,383,773 - Pf1N1B4_1219 0.88 -0.5 1,383,806 + -0.2 1,383,806 + -0.2 1,383,895 - -0.4 1,383,916 + -0.2 1,383,916 + -1.4 1,383,917 - +1.2 1,383,923 + +0.2 1,383,923 + +1.2 1,383,924 - +0.8 1,383,924 - -2.4 1,383,927 + +0.2 1,383,967 - +0.2 1,384,023 - +0.8 1,384,097 - -0.4 1,384,128 + -0.8 1,384,129 - -0.2 1,384,129 - -0.4 1,384,129 - -1.1 1,384,285 + Pf1N1B4_1220 0.18 +0.2 1,384,426 - Pf1N1B4_1220 0.34 -0.4 1,384,426 - Pf1N1B4_1220 0.34 +0.6 1,384,426 - Pf1N1B4_1220 0.34 -1.8 1,384,665 + Pf1N1B4_1220 0.61 +2.2 1,384,666 - Pf1N1B4_1220 0.62 +0.2 1,384,666 - Pf1N1B4_1220 0.62 -1.4 1,384,666 - Pf1N1B4_1220 0.62 +0.2 1,384,732 + Pf1N1B4_1220 0.69 +0.2 1,384,732 + Pf1N1B4_1220 0.69 -0.2 1,384,733 - Pf1N1B4_1220 0.69 +0.8 1,384,733 - Pf1N1B4_1220 0.69 -0.2 1,384,733 - Pf1N1B4_1220 0.69 -0.8 1,384,733 - Pf1N1B4_1220 0.69 +1.2 1,384,733 - Pf1N1B4_1220 0.69 -0.8 1,384,733 - Pf1N1B4_1220 0.69 +1.2 1,384,733 - Pf1N1B4_1220 0.69 -2.2 1,384,733 - Pf1N1B4_1220 0.69 -0.2 1,384,733 - Pf1N1B4_1220 0.69 -2.2 1,384,733 - Pf1N1B4_1220 0.69 +0.8 1,384,733 - Pf1N1B4_1220 0.69 +0.5 1,384,733 - +1.2 1,384,987 + +0.8 1,384,988 - -2.4 1,384,988 - -0.3 1,385,069 - -0.4 1,385,102 + +1.0 1,385,103 - +0.9 1,385,103 - -1.4 1,385,106 + +0.2 1,385,136 + +0.5 1,385,136 + +0.2 1,385,136 + +0.2 1,385,137 - +0.2 1,385,137 - +0.8 1,385,137 - -2.2 1,385,137 - +0.8 1,385,174 + +1.2 1,385,175 - +0.8 1,385,175 - +0.2 1,385,175 - -0.4 1,385,175 - +1.2 1,385,175 - -2.0 1,385,175 - +0.2 1,385,354 + Pf1N1B4_1221 0.17 -0.7 1,385,354 + Pf1N1B4_1221 0.17 -0.6 1,385,355 - Pf1N1B4_1221 0.17 +0.2 1,385,355 - Pf1N1B4_1221 0.17 +1.5 1,385,355 - Pf1N1B4_1221 0.17 +0.9 1,385,355 - Pf1N1B4_1221 0.17 -0.1 1,385,355 - Pf1N1B4_1221 0.17 -1.2 1,385,441 + Pf1N1B4_1221 0.25 -2.4 1,385,501 - Pf1N1B4_1221 0.30 -2.4 1,385,533 + Pf1N1B4_1221 0.33 -0.8 1,385,732 + Pf1N1B4_1221 0.50 +0.0 1,385,732 + Pf1N1B4_1221 0.50 +0.2 1,385,732 + Pf1N1B4_1221 0.50 -2.1 1,385,733 - Pf1N1B4_1221 0.50 -1.4 1,385,762 - Pf1N1B4_1221 0.53 -0.2 1,385,872 + Pf1N1B4_1221 0.62 +1.5 1,385,872 + Pf1N1B4_1221 0.62 -1.1 1,385,873 - Pf1N1B4_1221 0.62 -0.4 1,385,921 + Pf1N1B4_1221 0.66 +0.8 1,385,921 + Pf1N1B4_1221 0.66 +1.2 1,385,921 + Pf1N1B4_1221 0.66 +0.3 1,385,921 + Pf1N1B4_1221 0.66 +0.2 1,385,922 - -2.2
Or see this region's nucleotide sequence